Sökning: onr:"swepub:oai:lup.lub.lu.se:1b8e7c7b-a775-4099-bac1-db443a0d3e3d" >
Apolipoprotein M in...
-
Wang, MinThird Affiliated Hospital of Soochow University
(författare)
Apolipoprotein M induces inhibition of inflammatory responses via the S1PR1 and DHCR24 pathways
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
Nummerbeteckningar
-
LIBRIS-ID:oai:lup.lub.lu.se:1b8e7c7b-a775-4099-bac1-db443a0d3e3d
-
https://lup.lub.lu.se/record/1b8e7c7b-a775-4099-bac1-db443a0d3e3dURI
Kompletterande språkuppgifter
-
Språk:engelska
-
Sammanfattning på:engelska
Ingår i deldatabas
Klassifikation
-
Ämneskategori:art swepub-publicationtype
-
Ämneskategori:ref swepub-contenttype
Anmärkningar
-
© 2019 Spandidos Publications. All rights reserved. Apolipoprotein M (ApoM) is a type of apolipoprotein. It is well known that high-density lipoprotein (HDL) decreases inflammatory responses via the apoM-sphingosine-1-phosphate (S1P) pathway. The present study further investigated the importance of ApoM in the inhibitory effects of HDL on inflammation. Mice with an apoM gene deficiency (apoM-/-) were employed to investigate the effects of ApoM on the expression of interleukin-1β (IL-1β), monocyte chemotactic protein-1 (MCP-1), S1P receptor-1 (S1PR1) and 3β-hydroxysterol Δ-24-reductase (DHCR24), as compared with in wild-type mice (apoM+/+). Furthermore, cell culture experiments were performed using a permanent human hybrid endothelial cell line (EA.hy926). Cells were cultured in the presence of recombinant human apoM (rec-apoM) or were induced to overexpress apoM (apoMTg); subsequently, cells were treated with tumor necrosis factor-α (TNF-α), in order to investigate the effects of ApoM on IL-1β and MCP-1. The results demonstrated that the mRNA expression levels of IL-1β and MCP-1 were significantly higher in the liver following administration of lipopolysaccharide in apoM-/- mice compared with in apoM+/+ mice. In cell culture experiments, when cells were pre-cultured with rec-apoM or were engineered to overexpress apoM (apoMTg), they exhibited decreased expression levels of IL-1β and MCP-1 following TNF-α treatment compared with in normal apoM-expressing cells (apoMTgN). Furthermore, the mRNA expression levels of IL-1β and MCP-1 were significantly elevated following addition of the S1PR1 inhibitor W146, but not by the scavenger receptor class B type I inhibitor, block lipid transport-1 (BLT-1), in apoMTg cells prior to TNF-α treatment. Conversely, there were no differences in these inflammatory biomarkers under the same conditions in apoMTgN cells. The mRNA expression levels of DHCR24 were significantly reduced by the addition of BLT-1 prior to TNF-α treatment in apoMTg cells; however, there was no difference in the expression of this inflammatory biomarker in apoMTgN cells. In conclusion, ApoM displayed inhibitory effects against the inflammatory response in vivo and in vitro; these effects may be induced via the S1PR1 and DHCR24 pathways.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
-
Luo, Guang HuaThird Affiliated Hospital of Soochow University
(författare)
-
Liu, HongThird Affiliated Hospital of Soochow University
(författare)
-
Zhang, You PuThird Affiliated Hospital of Soochow University
(författare)
-
Wang, BinThird Affiliated Hospital of Soochow University
(författare)
-
Di, Dong MeiThird Affiliated Hospital of Soochow University
(författare)
-
Zhan, Xiang HongThird Affiliated Hospital of Soochow University
(författare)
-
Yu, YangThird Affiliated Hospital of Soochow University
(författare)
-
Yao, ShuangThird Affiliated Hospital of Soochow University
(författare)
-
Zhang, Xiao YingThird Affiliated Hospital of Soochow University
(författare)
-
Xu, NingLund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine(Swepub:lu)kkem-nxu
(författare)
-
Third Affiliated Hospital of Soochow UniversityAvdelningen för klinisk kemi och farmakologi
(creator_code:org_t)
Sammanhörande titlar
-
Ingår i:Molecular Medicine Reports19:2, s. 1272-12831791-2997
Internetlänk
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Wang, Min
-
Luo, Guang Hua
-
Liu, Hong
-
Zhang, You Pu
-
Wang, Bin
-
Di, Dong Mei
-
visa fler...
-
Zhan, Xiang Hong
-
Yu, Yang
-
Yao, Shuang
-
Zhang, Xiao Ying
-
Xu, Ning
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
- Artiklar i publikationen
-
Molecular Medici ...
- Av lärosätet
-
Lunds universitet