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Sökning: onr:"swepub:oai:lup.lub.lu.se:250d4506-cab6-4cf4-b8f3-01a7674e3c82" > Epigenetic programm...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00004488naa a2200553 4500
001oai:lup.lub.lu.se:250d4506-cab6-4cf4-b8f3-01a7674e3c82
003SwePub
008161003s2016 | |||||||||||000 ||eng|
0247 a https://lup.lub.lu.se/record/250d4506-cab6-4cf4-b8f3-01a7674e3c822 URI
0247 a https://doi.org/10.1007/s00125-016-4099-92 DOI
040 a (SwePub)lu
041 a engb eng
042 9 SwePub
072 7a art2 swepub-publicationtype
072 7a ref2 swepub-contenttype
1001 a Broholm, Christau Copenhagen University Hospital4 aut
2451 0a Epigenetic programming of adipose-derived stem cells in low birthweight individuals
264 c 2016-09-14
264 1b Springer Science and Business Media LLC,c 2016
300 a 10 s.
520 a Aims/hypothesis: Low birthweight (LBW) is associated with dysfunctions of adipose tissue and metabolic disease in adult life. We hypothesised that altered epigenetic and transcriptional regulation of adipose-derived stem cells (ADSCs) could play a role in programming adipose tissue dysfunction in LBW individuals. Methods: ADSCs were isolated from the subcutaneous adipose tissue of 13 normal birthweight (NBW) and 13 LBW adult men. The adipocytes were cultured in vitro, and genome-wide differences in RNA expression and DNA methylation profiles were analysed in ADSCs and differentiated adipocytes. Results: We demonstrated that ADSCs from LBW individuals exhibit multiple expression changes as well as genome-wide alterations in methylation pattern. Reduced expression of the transcription factor cyclin T2 encoded by CCNT2 may play a key role in orchestrating several of the gene expression changes in ADSCs from LBW individuals. Indeed, silencing of CCNT2 in human adipocytes decreased leptin secretion as well as the mRNA expression of several genes involved in adipogenesis, including MGLL, LIPE, PPARG, LEP and ADIPOQ. Only subtle genome-wide mRNA expression and DNA methylation changes were seen in mature cultured adipocytes from LBW individuals. Conclusions/interpretation: Epigenetic and transcriptional changes in LBW individuals are most pronounced in immature ADSCs that in turn may programme physiological characteristics of the mature adipocytes that influence the risk of metabolic diseases. Reduced expression of CCNT2 may play a key role in the developmental programming of adipose tissue.
650 7a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe
650 7a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng
653 a Adipocytes
653 a Adipogenesis
653 a Adipose tissue
653 a Cyclin T2
653 a Epigenetics
653 a Fetal programming
653 a Low birthweight
653 a Metabolic disease
653 a Type 2 diabetes
7001 a Olsson, Anders H.u Copenhagen University Hospital4 aut0 (Swepub:lu)med-a_o
7001 a Perfilyev, Alexanderu Lund University,Lunds universitet,Diabetes - epigenetik,Forskargrupper vid Lunds universitet,Diabetes - Epigenetics,Lund University Research Groups4 aut0 (Swepub:lu)med-apy
7001 a Hansen, Ninna S.u University of Copenhagen4 aut
7001 a Schrölkamp, Marenu Copenhagen University Hospital4 aut
7001 a Strasko, Klaudia S.u Copenhagen University Hospital4 aut
7001 a Scheele, Camillau Copenhagen University Hospital4 aut
7001 a Ribel-Madsen, Rasmusu Danish Diabetes Academy4 aut
7001 a Mortensen, Brynjulfu Steno Diabetes Center Copenhagen4 aut
7001 a Jørgensen, Sine W.u Steno Diabetes Center Copenhagen4 aut
7001 a Ling, Charlotteu Lund University,Lunds universitet,Diabetes - epigenetik,Forskargrupper vid Lunds universitet,Diabetes - Epigenetics,Lund University Research Groups4 aut0 (Swepub:lu)endo-cl0
7001 a Vaag, Allanu Steno Diabetes Center Copenhagen4 aut
7102 a Copenhagen University Hospitalb Diabetes - epigenetik4 org
7730 t Diabetologiad : Springer Science and Business Media LLCg 59:12, s. 2664-2673q 59:12<2664-2673x 0012-186Xx 1432-0428
8564 u http://dx.doi.org/10.1007/s00125-016-4099-9y FULLTEXT
8564 u https://link.springer.com/content/pdf/10.1007/s00125-016-4099-9.pdf
8564 8u https://lup.lub.lu.se/record/250d4506-cab6-4cf4-b8f3-01a7674e3c82
8564 8u https://doi.org/10.1007/s00125-016-4099-9

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