Sökning: onr:"swepub:oai:lup.lub.lu.se:cd3b2f40-bf8c-4e34-9753-d4eaafc2253d" > Assembly of human c...
Fältnamn | Indikatorer | Metadata |
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000 | 03647naa a2200289 4500 | |
001 | oai:lup.lub.lu.se:cd3b2f40-bf8c-4e34-9753-d4eaafc2253d | |
003 | SwePub | |
008 | 160401s1996 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/311052 URI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Ben Nasr, Abdelhakim4 aut |
245 | 1 0 | a Assembly of human contact phase factors and release of bradykinin at the surface of curli-expressing Escherichia coli |
264 | 1 | c 1996 |
520 | a Previous work has demonstrated that most strains of the human pathogen Streptococcus pyogenes bind kininogens through M protein, a fibrous surface protein and virulence determinant. Here we find that strains of several other pathogenic bacterial species, both Gram-positive and Gram-negative, isolated from patients with sepsis, also bind kininogens, especially kininogen (HK). The most pronounced interaction was seen between HK and Escherichia coli. Among clinical isolates of E. coli, the majority of the enterohaemorrhagic, enterotoxigenic, and sepsis strains, but none of the enteroinvasive and enteropathogenic strains, bound HK. Binding of HK to E. coli correlated with the expression of curli, another fibrous bacterial surface protein, and the binding of HK to purified curli was specific, saturable, and of high affinity; Ka = 9 x 10(7) M-1. Other contact phase proteins such as factor XI, factor XII, and prekallikrein bound to curliated E. coli, but not to an isogenic curli-deficient mutant strain, suggesting that contact phase activation may occur at the surface of curliated bacteria. Kininogens are also precursor molecules of the vasoactive kinins. When incubated with human plasma, curli-expressing bacteria absorbed HK. Addition of purified plasma kallikrein to the HK-loaded bacteria resulted in a rapid and efficient release of bradykinin from surface-bound HK. The assembly of contact phase factors at the surface of pathogenic bacteria and the release of the potent proinflammatory and vasoactive peptide bradykinin, should have a major impact on the host-microbe relationship and may contribute to bacterial pathogenicity and virulence. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Mikrobiologi inom det medicinska området0 (SwePub)301092 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Microbiology in the medical area0 (SwePub)301092 hsv//eng |
700 | 1 | a Olsén, Arneu Lund University,Lunds universitet,Institutionen för experimentell medicinsk vetenskap,Medicinska fakulteten,Department of Experimental Medical Science,Faculty of Medicine4 aut0 (Swepub:lu)medk-aol |
700 | 1 | a Sjöbring, Ulfu Lund University,Lunds universitet,Avdelningen för mikrobiologi, immunologi och glykobiologi - MIG,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Microbiology, Immunology and Glycobiology - MIG,Department of Laboratory Medicine,Faculty of Medicine4 aut0 (Swepub:lu)mmb-usj |
700 | 1 | a Müller-Esterl, Werner4 aut |
700 | 1 | a Björck, Larsu Lund University,Lunds universitet,Infektionsmedicin,Sektion III,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Infection Medicine (BMC),Section III,Department of Clinical Sciences, Lund,Faculty of Medicine4 aut0 (Swepub:lu)medk-lbj |
710 | 2 | a Institutionen för experimentell medicinsk vetenskapb Medicinska fakulteten4 org |
773 | 0 | t Molecular Microbiologyg 20:5, s. 35-927q 20:5<35-927x 1365-2958 |
856 | 4 8 | u https://lup.lub.lu.se/record/31105 |
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