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Sökning: onr:"swepub:oai:prod.swepub.kib.ki.se:145437100" > XPR1 Mediates the P...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00002842naa a2200373 4500
001oai:prod.swepub.kib.ki.se:145437100
003SwePub
008240919s2021 | |||||||||||000 ||eng|
024a http://kipublications.ki.se/Default.aspx?queryparsed=id:1454371002 URI
024a https://doi.org/10.2337/db19-06332 DOI
040 a (SwePub)ki
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Barker, CJu Karolinska Institutet4 aut
2451 0a XPR1 Mediates the Pancreatic β-Cell Phosphate Flush
264 c 2020-08-21
264 1b American Diabetes Association,c 2021
520 a Glucose-stimulated insulin secretion is the hallmark of the pancreatic β-cell, a critical player in the regulation of blood glucose concentration. In 1974, the remarkable observation was made that an efflux of intracellular inorganic phosphate (Pi) accompanied the events of stimulated insulin secretion. The mechanism behind this “phosphate flush,” its association with insulin secretion, and its regulation have since then remained a mystery. We recapitulated the phosphate flush in the MIN6m9 β-cell line and pseudoislets. We demonstrated that knockdown of XPR1, a phosphate transporter present in MIN6m9 cells and pancreatic islets, prevented this flush. Concomitantly, XPR1 silencing led to intracellular Pi accumulation and a potential impact on Ca2+ signaling. XPR1 knockdown slightly blunted first-phase glucose-stimulated insulin secretion in MIN6m9 cells, but had no significant impact on pseudoislet secretion. In keeping with other cell types, basal Pi efflux was stimulated by inositol pyrophosphates, and basal intracellular Pi accumulated following knockdown of inositol hexakisphosphate kinases. However, the glucose-driven phosphate flush occurred despite inositol pyrophosphate depletion. Finally, while it is unlikely that XPR1 directly affects exocytosis, it may protect Ca2+ signaling. Thus, we have revealed XPR1 as the missing mediator of the phosphate flush, shedding light on a 45-year-old mystery.
700a Tessaro, FHG4 aut
700a Ferreira, SD4 aut
700a Simas, R4 aut
700a Ayala, TS4 aut
700a Kohler, Mu Karolinska Institutet4 aut
700a Rajasekaran, SS4 aut
700a Martins, JO4 aut
700a Dare, Eu Karolinska Institutet4 aut
700a Berggren, POu Karolinska Institutet4 aut
710a Karolinska Institutet4 org
773t Diabetesd : American Diabetes Associationg 70:1, s. 111-118q 70:1<111-118x 1939-327Xx 0012-1797
856u https://diabetesjournals.org/diabetes/article-pdf/70/1/111/437815/db190633.pdf
8564 8u http://kipublications.ki.se/Default.aspx?queryparsed=id:145437100
8564 8u https://doi.org/10.2337/db19-0633

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