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Presence of IGF-I i...
Presence of IGF-I in human midgut carcinoid tumours--an autocrine regulator of carcinoid tumour growth?
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Nilsson, Ola, 1957 (författare)
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- Wängberg, Bo, 1953 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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Theodorsson, E (författare)
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visa fler...
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Skottner, A (författare)
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- Ahlman, Håkan, 1947 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för de kirurgiska disciplinerna, Avdelningen för kirurgi,Institute of Surgical Sciences, Department of Surgery
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visa färre...
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(creator_code:org_t)
- 1992
- 1992
- Engelska.
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Ingår i: International journal of cancer. Journal international du cancer. - 0020-7136. ; 51:2, s. 195-203
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- The presence of IGF-I and IGF-I receptors in human midgut carcinoid tumours has been investigated. Using immunocytochemistry, IGF-I-positive tumour cells were demonstrated in 11/11 tumour cases studied. Labelling of consecutive sections with antibodies against IGF-I and proliferating cell nuclear antigen (PCNA)/cyclin demonstrated a co-distribution of the 2 antigens in carcinoid tumours. Extracts of tumour tissues were subjected to radioimmunoassay and shown to contain significant amounts of IGF-I. Reverse-phase HPLC of tumour extracts demonstrated a major IGF-I-immunoreactive component eluting in the position of rhIGF-I, but also 2 other more hydrophobic forms. Conditioned serum-free media from primary cultures of carcinoid tumors contained detectable amounts of IGF-I, indicating a spontaneous release of IGF-I from tumour cells into the culture medium. Levels of IGF-I in media were reduced (19%) after incubation of cultures with a somatostatin analogue for 4 days. IGF-I receptors were observed on tumour cells in 4/10 tumours by immunocytochemistry. Tumour cells with immunoreactive IGF-I receptors could be stimulated to enhanced growth, measured as an increase in DNA contents, by exogenous administration of IGF-I every 3-4 days for 2 weeks. The results show that cultured human midgut carcinoid tumours secrete IGF-I and that some of the tumours also have IGF-I receptors. We therefore suggest that IGF-I may act as an autocrine or paracrine regulator of carcinoid tumour-cell growth.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Nyckelord
- Adult
- Aged
- Carcinoid Tumor
- chemistry
- secretion
- Chromatography
- High Pressure Liquid
- DNA
- analysis
- Female
- Humans
- Ileal Neoplasms
- chemistry
- secretion
- Immunohistochemistry
- Insulin-Like Growth Factor I
- analysis
- genetics
- secretion
- Male
- Middle Aged
- Receptors
- Cell Surface
- analysis
- genetics
- Receptors
- Somatomedin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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