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Microcalorimetry as...
Microcalorimetry as a tool for the detection of complement-dependent cytotoxicity
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- Ankerst, J. (författare)
- Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Wallenberglaboratoriet, Lund,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,The Wallenberg Laboratory
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- Fäldt, R. (författare)
- Lund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Wallenberglaboratoriet, Malmö,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,The Wallenberg Laboratory, Malmö
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- Sjögren, H. O. (författare)
- Lund University,Lunds universitet,Wallenberglaboratoriet, Lund,Medicinska fakulteten,The Wallenberg Laboratory,Faculty of Medicine
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(creator_code:org_t)
- Elsevier BV, 1985
- 1985
- Engelska.
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Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 77:2, s. 267-274
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Microcalorimetry using a 4-channel static ampoule microcalorimeter of thermopile type has been evaluated as a tool for the detection of complement-dependent cytotoxicity against the surface antigens of living cells. Cytotoxic reactions mediated by a rabbit antiserum against human white blood cells and by 2 different monoclonal antibodies recognizing a melanoma-associated antigen on a human melanoma cell line were studied. The cytotoxic reactions were registered as a decrease of the heat production rate when the cells were exposed to antibodies in the presence of active complement as compared to the heat production rate of the cells exposed to the same antibodies in the presence of inactive complement. This investigation shows that microcalorimetry can be used as a highly sensitive method for the detection of complement-dependent immune reactions, detecting antibody dilutions higher than 10-5. It also indicates that microcalorimetry may become a particularly important technique in the analysis of the kinetics of cytotoxic immune reactions in vitro.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Hematologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Hematology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- complement-dependent cytotoxicity
- heat production rate
- microcalorimetry
- monoclonal antibody
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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