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Strategic use of an...
Strategic use of an adenoviral vector for rapid and efficient ex vivo-generation of cytomegalovirus pp65-reactive cytolytic and helper T cells
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Forsberg, Ole 1979- (författare)
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Carlsson, Björn (författare)
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Tötterman, Thomas H. (författare)
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Essand, Magnus (författare)
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- 2008
- 2008
- Engelska.
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Ingår i: British Journal of Haematology. - 0007-1048. ; 141:2, 188-199
Abstract
Ämnesord
Stäng
- Cytomegalovirus (CMV) reactivation can cause severe complications for transplant patients. Such patients can be protected against CMV-associated diseases through reconstitution of donor-derived CMV-reactive cytolytic and helper T cells. We have developed a strategic protocol for efficient simultaneous generation of CMV-reactive CD8 + and CD4 + T cells ex vivo . The protocol uses peripheral blood lymphocytes (PBLs), antigen-modified mature dendritic cells (DCs) generated in only 3 days and an adenoviral vector encoding the CMV pp65 antigen (Adpp65) both as an endogenous and exogenous source of antigen. PBLs stimulated once with Adpp65-transduced DCs (endogenously expressed pp65) resulted in preferential activation and expansion of pp65-specific CD8 + T cells while PBLs stimulated with DCs pulsed with cell lysate from Adpp65-transduced autologous monocytes (exogenously expressed pp65) yielded pp65-specific CD4 + T cells. Stimulation with double-modified DCs efficiently activated and expanded cytolytic and helper T cells simultaneously. The frequency of T cells producing interferon (IFN)-γ in response to pp65 increased after one stimulation on average 9.6-fold to 4.3% for CD8 + T cells and 25.8-fold to 6.5% for CD4 + T cells. This implies that sufficient number of pp65-specific cytolytic and helper T cells for adoptive transfer may be obtained in only two weeks.
Ämnesord
- Medical and Health Sciences (hsv)
- Basic Medicine (hsv)
- Microbiology in the medical area (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinska grundvetenskaper (hsv)
- Mikrobiologi inom det medicinska området (hsv)
- Medical and Health Sciences (hsv)
- Basic Medicine (hsv)
- Immunology in the medical area (hsv)
- Medicin och hälsovetenskap (hsv)
- Medicinska grundvetenskaper (hsv)
- Immunologi inom det medicinska området (hsv)
- MEDICINE (svep)
- Microbiology, immunology, infectious diseases (svep)
- MEDICIN (svep)
- Mikrobiologi, immunologi, infektionssjukdomar (svep)
- MEDICINE (svep)
- Microbiology, immunology, infectious diseases (svep)
- Immunology (svep)
- Clinical immunology (svep)
- MEDICIN (svep)
- Mikrobiologi, immunologi, infektionssjukdomar (svep)
- Immunologi (svep)
- Klinisk immunologi (svep)
- Klinisk immunologi (uu)
- Clinical Immunology (uu)
Nyckelord
- Dendritic cells
- T lymphocytes
- Cytomegalovirus
- Adenoviral vector
- pp65
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