Protective DNA vaccination against experimental autoimmune encephalomyelitis is associated with induction of IFNbeta

• DNA vaccines encoding encephalitogenic peptides protect against subsequent development of rat experimental autoimmune encephalomyelitis (EAE) through unknown mechanisms. We investigated immune cell phenotypes at different time points after DNA vaccination with vaccine encoding myelin oligodendrocyte glycoprotein peptide 91-108 and subsequent induction of EAE. In protected rats, we observed (i) no alterations in antigen-specific Th2 or Th3 responses, (ii) reduced MHC II expression on splenocytes early after EAE induction, (iii) antigen-specific upregulation of IFNbeta upon recall stimulation and (iv) reduced IL-12Rbeta2 on lymphocytes. We suggest that the underlying mechanism of DNA vaccination is associated with immunomodulation exerted by induced IFNbeta.

Nyckelord

Animals

Antigens; Surface/genetics/metabolism

Cells; Cultured

Central Nervous System/cytology

Comparative Study

Cytokines/genetics/metabolism

Disease Models; Animal

Encephalomyelitis; Autoimmune; Experimental/immunology/*prevention & control

Flow Cytometry/methods

Gene Expression Regulation/immunology

Humans

Interferon-beta/*biosynthesis/genetics

Lectins; C-Type/genetics/metabolism

Lipopolysaccharides/pharmacology

Lymphocytes/immunology/metabolism

Major Histocompatibility Complex/genetics

Mice

Myelin-Associated Glycoprotein/immunology

Peptide Fragments/immunology

RNA; Messenger/biosynthesis

Rats

Rats; Inbred Lew

Receptors; Interleukin/genetics/metabolism

Receptors; Virus/genetics/metabolism

Research Support; Non-U.S. Gov't

Reverse Transcriptase Polymerase Chain Reaction/methods

Sequence Alignment

T-Lymphocytes/drug effects/immunology

Time Factors

Vaccination

Vaccines; DNA/*administration & dosage/immunology

MEDICINE

MEDICIN

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