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OX1 orexin receptor...
OX1 orexin receptors activate extracellular signal-regulated kinase in Chinese hamster ovary cells via multiple mechanisms : the role of Ca2+ influx in OX1 receptor signaling
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- Ammoun, Sylwia (författare)
- Uppsala universitet,Fysiologi
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- Johansson, Lisa (författare)
- Uppsala universitet,Fysiologi
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- Ekholm, Marie E. (författare)
- Uppsala universitet,Fysiologi
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- Holmqvist, Tomas (författare)
- Uppsala universitet,Fysiologi
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- Danis, Alexander S. (författare)
- Uppsala universitet,Fysiologi
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- Korhonen, Laura (författare)
- Uppsala universitet,Institutionen för neurovetenskap
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- Sergeeva, Olga A. (författare)
- Uppsala universitet,Fysiologi
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Haas, Helmut L. (författare)
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- Åkerman, Karl E. O. (författare)
- Uppsala universitet,Fysiologi
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- Kukkonen, Jyrki P. (författare)
- Uppsala universitet,Fysiologi
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(creator_code:org_t)
- The Endocrine Society, 2006
- 2006
- Engelska.
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Ingår i: Molecular Endocrinology. - : The Endocrine Society. - 0888-8809 .- 1944-9917. ; 20:1, s. 80-99
- Relaterad länk:
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https://academic.oup...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Activation of OX1 orexin receptors heterologously expressedin Chinese hamster ovary (CHO) cells led to a rapid, strong,and long-lasting increase in ERK phosphorylation (activation).Dissection of the signal pathways to ERK using multiple inhibitorsand dominant-negative constructs indicated involvement of Ras,protein kinase C, phosphoinositide-3-kinase, and Src. Most interestingly,Ca2+ influx appeared central for the ERK response in CHO cells,and the same was indicated in recombinant neuro-2a cells andcultured rat striatal neurons. Detailed investigations in CHOcells showed that inhibition of the receptor- and store-operatedCa2+ influx pathways could fully attenuate the response, whereasinhibition of the store-operated Ca2+ influx pathway alone orthe Ca2+ release was ineffective. If the receptor-operated pathwaywas blocked, an exogenously activated store-operated pathwaycould take its place and restore the coupling of OX1 receptorsto ERK. Further experiments suggested that Ca2+ influx, as such,may not be required for ERK phosphorylation, but that Ca2+,elevated via influx, acts as a switch enabling OX1 receptorsto couple to cascades leading to ERK phosphorylation, cAMP elevation,and phospholipase C activation. In conclusion, the data suggestthat the primary coupling of orexin receptors to Ca2+ influxallows them to couple to other signal pathways; in the absenceof coupling to Ca2+ influx, orexin receptors can act as signalintegrators by taking advantage of other Ca2+ influx pathways.
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Till lärosätets databas
- Av författaren/redakt...
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Ammoun, Sylwia
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Johansson, Lisa
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Ekholm, Marie E.
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Holmqvist, Tomas
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Danis, Alexander ...
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Korhonen, Laura
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visa fler...
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Sergeeva, Olga A ...
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Haas, Helmut L.
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Åkerman, Karl E. ...
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Kukkonen, Jyrki ...
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visa färre...
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Molecular Endocr ...
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Uppsala universitet