Tyck till om SwePub Sök
här!
Sökning: onr:"swepub:oai:lup.lub.lu.se:f364d898-61a3-41c0-9bb8-4c8081601383" >
Time-resolved fluor...
Time-resolved fluorescence imaging (TRFI) for direct immunofluorescence of PSA and alpha-1-antichymotrypsin in prostatic tissue sections
-
- Bjartell, A. (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine,Skåne University Hospital
-
- Siivola, P. (författare)
- University of Turku
-
- Hulkko, S. (författare)
- University of Turku
-
visa fler...
-
- Pettersson, K. (författare)
- University of Turku
-
- Rundt, K. (författare)
- Wallac Oy
-
- Lilja, H. (författare)
- Lund University,Lunds universitet,Medicinska fakulteten,Faculty of Medicine,Skåne University Hospital
-
- Lövgren, T. (författare)
- University of Turku
-
visa färre...
-
(creator_code:org_t)
- 1999-09-13
- 1999
- Engelska 8 s.
-
Ingår i: Prostate Cancer and Prostatic Diseases. - : Springer Science and Business Media LLC. - 1365-7852 .- 1476-5608. ; 2:3, s. 140-147
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
https://www.nature.c...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- We have developed a direct immunofluorescence technique utilising chelates of the lanthanide ions europium and terbium conjugated to monoclonal IgGs (Mabs) against prostate-specific antigen (PSA) and alpha-1- antichymotrypsin (ACT) for the detection and quantification on the same tissue section. Strong signals without disturbance from tissue autofluorescence were demonstrated in paraffin sections of ten benign and six malignant prostate tissue specimens. The signal intensity increased linearly with the amount of labelled Mab until epitope saturation began. The highest concentrations of bound IgG in tissue sections were 27.3 fmol/pixel for ACT and 7.2 for PSA. Time-resolved fluorescence imaging (TRFI) offers an attractive method for histochemical studies based on specific and quantitative detection of fluorescent lanthanide chelates.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Läkemedelskemi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medicinal Chemistry (hsv//eng)
Nyckelord
- Europium
- Image analysis
- Immunohistochemistry
- Lanthanide chelates
- Prostate cancer
- Terbium
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas