Probing Physical Properties of a DNA- Protein Complex Using Nanofluidic Channels

• A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.

Subject headings

NATURVETENSKAP  -- Biologi -- Biofysik (hsv//swe)

NATURAL SCIENCES  -- Biological Sciences -- Biophysics (hsv//eng)

Keyword

DNA

fluorescence

lipid passivation

nanofluidics

proteins

Publication and Content Type

ref (subject category)

art (subject category)