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Genomic mining of G...
Genomic mining of Geobacillus stearothermophilus GF16 for xylose production from hemicellulose-rich biomasses using secreted enzymes
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- Carbonaro, Miriam (författare)
- Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II
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- Aulitto, Martina, 1991 (författare)
- Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II
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- Mazurkewich, Scott, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fraia, Alessia Di (författare)
- Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II
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- Contursi, Patrizia (författare)
- Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II
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- Limauro, Danila (författare)
- Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II
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- Larsbrink, Johan, 1982 (författare)
- Chalmers tekniska högskola,Chalmers University of Technology
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- Fiorentino, Gabriella (författare)
- Universita Degli Studi Di Napoli Federico Ii,University of Naples Federico II
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(creator_code:org_t)
- 2024
- 2024
- Engelska.
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Ingår i: New Biotechnology. - 1876-4347 .- 1871-6784. ; 82, s. 14-24
- Relaterad länk:
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https://research.cha... (primary) (free)
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https://doi.org/10.1...
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https://research.cha...
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Abstract
Ämnesord
Stäng
- The valorization of lignocellulosic biomass, derived from various bio-waste materials, has received considerable attention as a sustainable approach to improve production chains while reducing environmental impact. Microbial enzymes have emerged as key players in the degradation of polysaccharides, offering versatile applications in biotechnology and industry. Among these enzymes, glycoside hydrolases (GHs) play a central role. Xylanases, in particular, are used in a wide range of applications and are essential for the production of xylose, which can be fermented into bioethanol or find use in many other industries. Currently, fungal secretomes dominate as the main reservoir of lignocellulolytic enzymes, but thermophilic microorganisms offer notable advantages in terms of enzyme stability and production efficiency. Here we present the genomic characterization of Geobacillus stearothermophilus GF16 to identify genes encoding putative enzymes involved in lignocellulose degradation. Thermostable GHs secreted by G. stearothermophilus GF16 were investigated and found to be active on different natural polysaccharides and synthetic substrates, revealing an array of inducible GH activities. In particular, the concentrated secretome possesses significant thermostable xylanase and β-xylosidase activities (5 ×103 U/L and 1.7 ×105 U/L, respectively), highlighting its potential for application in biomass valorization. We assessed the hemicellulose hydrolysis capabilities of various agri-food wastes using the concentrated secretome of the strain cultivated on xylan. An impressive 300-fold increase in xylose release compared to a commercially available cocktail was obtained with the secretome, underscoring the remarkable efficacy of this approach.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Bioenergi (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Bioenergy (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Industriell bioteknik -- Biokatalys och enzymteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Industrial Biotechnology -- Biocatalysis and Enzyme Technology (hsv//eng)
Nyckelord
- Geobacillus stearothermophilus GF16
- Xylan
- Carbohydrate active enzymes
- Agri-food waste valorization
- Bacterial secretome
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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