Functionalized Nanostructures: Redox-Active Porphyrin Anchors for Supramolecular DNA Assemblies

• We have synthesized and studied a supramolecular system comprising a 39-mer DNA with porphyrin-modified thymidine nucleosides anchored to the surface of large unilamellar vesicles (liposomes). Liposome porphyrin binding characteristics, such as orientation, strength, homogeneity, and binding site size, was determined, suggesting that the porphyrin is well suited as a photophysical and redox-active lipid anchor, in comparison to the inert cholesterol anchor commonly used today. Furthermore, the binding characteristics and hybridization capabilities were studied as a function of anchor size and number of anchoring points, properties that are of importance for our future plans to use the addressability of these redox-active nodes in larger DNA-based nanoconstructs. Electron transfer from photoexcited porphyrin to a lipophilic benzoquinone residing in the lipid membrane was characterized by steady-state and time-resolved fluorescence and verified by femtosecond transient absorption.

Ämnesord

NATURVETENSKAP  -- Kemi (hsv//swe)

NATURAL SCIENCES  -- Chemical Sciences (hsv//eng)

Nyckelord

electron

dependence

transfer

membrane

porphyrin

supramolecular

DNA

nanotechnology

construction

scaffold

bridge-acceptor systems

flow

orientation

fluorescence

dichroism

liposomes

membrane

photoinduced electron-transfer

Publikations- och innehållstyp

art (ämneskategori)

ref (ämneskategori)