SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Folkersen Lasse)
 

Sökning: WFRF:(Folkersen Lasse) > Phenotype and genot...

Phenotype and genotype effects on the transcriptome in cardiovascular disease : tools to identify candidate genes

Folkersen, Lasse (författare)
 
 
ISBN 9789174575842
Stockholm : Karolinska Institutet, Dept of Medicine, Solna, 2011
Engelska.
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)
Abstract Ämnesord
Stäng  
  • The overarching purpose of this thesis was to investigate the expression of human genes and how they relate to cardiovascular disease. This was pursued through five papers, each of which investigated different aspects of gene expression. Paper I improved the technology for extracting gene expression information from microarrays. Its main purpose was to introduce the software package GeneRegionScan, which was developed for analysis of genomic regions with inaccurate annotation. Paper II explored the genomic region surrounding the first identified single‐ nucleotide polymorphism (SNP) associated with cardiovascular disease. The purpose of the paper was to investigate transcript isoforms of the regional genes and their association to the risk‐SNPs in the region. Eight new transcript isoforms were introduced, but no pattern of association with genotype was observed. Paper III extended the methods of paper II, by expanding the search to 166 risk‐ SNPs known at the time. For each of these SNPs, it was hypothesized that one or more proximal genes had expression levels that were associated to the genotype of the SNP in question. It was reported that 47 of the SNPs had such genes, thus progressing from risk‐SNPs towards risk‐genes. In addition the paper introduced new concepts on distance between gene and risk SNP and on the tissue‐ specificity of associations. Paper IV investigated the gene expression pattern of thoracic aortic aneurysm. It had previously been observed that patients with congenital bicuspid aortic valve (BAV) had increased severity and earlier onset age of aneurysm, when compared to patients with the normal tricuspid aortic valve (TAV). A fundamentally differing gene expression profile was observed between these two patient groups, and a possible immunological involvement in TAV patients was reported. Paper V asked if the high‐throughput methods of genomics and transcriptomics could be applied towards better prediction of future ischemic events in patients with established atherosclerosis. Risk‐SNP profiles, gene expression profiles of circulating blood cells, and gene expression profiles of carotid plaque samples were utilized. Particularly gene expression profiles of carotid plaque provided improved prediction above that of the established risk markers of serum lipids, gender, age and smoking.

Publikations- och innehållstyp

vet (ämneskategori)
dok (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Hitta mer i SwePub

Av författaren/redakt...
Folkersen, Lasse
Av lärosätet
Karolinska Institutet

Sök utanför SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy