SwePub
Sök i LIBRIS databas

  Utökad sökning

WFRF:(Hauling Thomas)
 

Sökning: WFRF:(Hauling Thomas) > A Drosophila immune...

A Drosophila immune response against Ras-induced overgrowth

Hauling, Thomas (författare)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
Krautz, Robert (författare)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
Markus, Robert (författare)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
visa fler...
Volkenhoff, Anne (författare)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
Kucerova, Lucie (författare)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
Theopold, Ulrich (författare)
Stockholms universitet,Institutionen för molekylär biovetenskap, Wenner-Grens institut
visa färre...
 (creator_code:org_t)
2014-03-21
2014
Engelska.
Ingår i: Biology Open. - : The Company of Biologists. - 2046-6390. ; 3:4, s. 250-260
  • Tidskriftsartikel (refereegranskat)
Abstract Ämnesord
Stäng  
  • Our goal is to characterize the innate immune response against the early stage of tumor development. For this, animal models where genetic changes in specific cells and tissues can be performed in a controlled way have become increasingly important, including the fruitfly Drosophila melanogaster. Many tumor mutants in Drosophila affect the germline and, as a consequence, also the immune system itself, making it difficult to ascribe their phenotype to a specific tissue. Only during the past decade, mutations have been induced systematically in somatic cells to study the control of tumorous growth by neighboring cells and by immune cells. Here we show that upon ectopic expression of a dominant-active form of the Ras oncogene (Ras(V12)), both imaginal discs and salivary glands are affected. Particularly, the glands increase in size, express metalloproteinases and display apoptotic markers. This leads to a strong cellular response, which has many hallmarks of the granuloma-like encapsulation reaction, usually mounted by the insect against larger foreign objects. RNA sequencing of the fat body reveals a characteristic humoral immune response. In addition we also identify genes that are specifically induced upon expression of Ras(V12). As a proof-of-principle, we show that one of the induced genes (santa-maria), which encodes a scavenger receptor, modulates damage to the salivary glands. The list of genes we have identified provides a rich source for further functional characterization. Our hope is that this will lead to a better understanding of the earliest stage of innate immune responses against tumors with implications for mammalian immunity.

Ämnesord

NATURVETENSKAP  -- Biologi (hsv//swe)
NATURAL SCIENCES  -- Biological Sciences (hsv//eng)

Nyckelord

Innate immunity
Tumor
Oncogene
Insect immunity
Hemocytes
Encapsulation
Molecular Biology
molekylärbiologi

Publikations- och innehållstyp

ref (ämneskategori)
art (ämneskategori)

Hitta via bibliotek

Till lärosätets databas

Sök utanför SwePub

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy