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MCLIP Detection of ...
MCLIP Detection of Novel Protein-Protein Interactions at the Nuclear Envelope
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- Jafferali, Mohammed Hakim (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Figueroa, Ricardo A. (författare)
- Stockholms universitet,Institutionen för neurokemi
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- Hallberg, Einar (författare)
- Stockholms universitet,Institutionen för neurokemi
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(creator_code:org_t)
- Elsevier, 2016
- 2016
- Engelska.
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Ingår i: Intermediate Filament Associated Proteins. - : Elsevier. - 9780128034699 ; , s. 503-515
- Relaterad länk:
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https://urn.kb.se/re...
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visa fler...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- The organization and function of the nuclear envelope (NE) involves hundreds of nuclear membrane proteins and myriad protein-protein interactions, most of which are still uncharacterized. Many NE proteins interact stably or dynamically with the nuclear lamina or chromosomes. This can make them difficult to extract under non-denaturing conditions, and greatly limits our ability to explore and identify functional protein interactions at the NE. This knowledge is needed to understand nuclear envelope structure and the mechanisms of human laminopathy diseases. This chapter provides detailed protocols for MCLIP (membrane cross-linking immunoprecipitation) identification of novel protein-protein interactions in mammalian cells.
Ämnesord
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Nucleus
- Nuclear envelope
- Nuclear lamina
- Nuclear membrane
- Protein–protein interactions
- Proteomics
- MCLIP
Publikations- och innehållstyp
- ref (ämneskategori)
- kap (ämneskategori)
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