Sökning: WFRF:(Joniau Steven) >
Intratunical Inject...
Intratunical Injection of Human Adipose Tissue–Derived Stem Cells Restores Collagen III/I Ratio in a Rat Model of Chronic Peyronie's Disease
-
- Castiglione, Fabio (författare)
- University College London Hospital,San Raffaele Hospital,Catholic University of Leuven,Univ Leuven, Belgium; Univ Coll London Hosp, England; IRCCS Osped San Raffaele, Italy
-
- Hedlund, Petter (författare)
- Linköpings universitet,Linköping University,Lund University,Lunds universitet,Avdelningen för klinisk kemi och farmakologi,Institutionen för laboratoriemedicin,Medicinska fakulteten,Division of Clinical Chemistry and Pharmacology,Department of Laboratory Medicine,Faculty of Medicine,Avdelningen för läkemedelsforskning,Region Östergötland, Klinisk farmakologi,Lund Univ, Sweden
-
- Weyne, Emanuel (författare)
- Catholic University of Leuven,Univ Leuven, Belgium
-
visa fler...
-
- Hakim, Lukman (författare)
- Catholic University of Leuven,Dr. Soetomo Hospital,Arilangga University,Univ Leuven, Belgium; Airlangga Univ, Indonesia
-
- Montorsi, Francesco (författare)
- San Raffaele Hospital,IRCCS Osped San Raffaele, Italy
-
- Bivalacqua, Trinity J. (författare)
- Johns Hopkins Hospital,Johns Hopkins Med Inst, MD 21205 USA
-
- De Ridder, Dirk (författare)
- Catholic University of Leuven,Univ Leuven, Belgium
-
- Milenkovic, Uros (författare)
- Catholic University of Leuven,Univ Leuven, Belgium
-
- Ralph, David (författare)
- University College London Hospital,Univ Coll London Hosp, England
-
- Garaffa, Giulio (författare)
- University College London Hospital,Univ Coll London Hosp, England
-
- Muneer, Asif (författare)
- University College London Hospital,Univ Coll London Hosp, England
-
- Joniau, Steven (författare)
- Catholic University of Leuven,Univ Leuven, Belgium
-
- Albersen, Maarten (författare)
- Catholic University of Leuven
-
visa färre...
-
(creator_code:org_t)
-
- 2018-11-29
- 2019
- Engelska.
-
Ingår i: Sexual Medicine. - : Oxford University Press (OUP). - 2050-1161. ; 7:1, s. 94-103
- Relaterad länk:
-
http://dx.doi.org/10... (free)
-
visa fler...
-
http://www.smoa.jsex...
-
https://liu.diva-por... (primary) (Raw object)
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
visa färre...
Abstract
Ämnesord
Stäng
- Introduction: Previous studies have shown that the injection of adipose tissue–derived stem cells (ADSCs) into the tunica albuginea (TA) during the active phase of Peyronie's disease (PD) prevents the development of fibrosis. Aim: To investigate, using an animal model, whether local injection of human ADSCs (hADSCs) can alter the degree of fibrosis in the chronic phase of PD. Methods: 27 male, 12-week-old rats were divided into 3 equal groups: sham, PD without treatment, and PD treated with hADSCs 1 month after disease induction. Sham rats underwent 2 injections of vehicle into the TA 1 month apart. PD rats underwent transforming growth factor β1 (TGFβ1) injection and injection of vehicle 1 month later. PD-hADSC rats underwent TGFβ1 injection followed by 1 million hADSCs 1 month later. 1 week after treatment, n = 3 animals/group were euthanized, and the penises were harvested for quantitative polymerase chain reaction. 1 month after treatment, the other animals, n = 6 per group, underwent measurement of intracavernous pressure (ICP) and mean arterial pressure (MAP) during electrostimulation of the cavernous nerve. After euthanasia, penises were again harvested for histology and Western blot. Main Outcome Measure: The primary outcome measures included (a) gene expression at one week post-injection; (b) measurement of ICP/MAP upon cavernous nerve stimulation as a measure of erectile function; (c) elastin, collagen I and III protein expression; and (d) Histomorphometric analysis of the penis. Means where compared by analysis of variance (ANOVA) followed by a Student-Newman-Keuls test for post hoc comparisons or Mann-Whitney test when applicable. Results: No significant difference was noted in ICP or ICP/MAP in response to cavernous nerve electrostimulation between the 3 groups at 2.5, 5, and 7.5 V (P > .05 for all voltages). PD animals developed tunical and subtunical areas of fibrosis with a significant upregulation of collagen III protein. The collagen III/I ratio was higher in the PD (4.6 ± 0.92) group compared with sham (0.66 ± 0.18) and PD-hADSC (0.86 ± 0.06) groups (P < .05) These fibrotic changes were prevented when treated with hADSCs. Compared with PD rats, PD-hADSC rats demonstrated a decreased expression of several fibrosis-related genes. Conclusion: Injection of hADSCs reduces collagen III expression in a rat model of chronic PD. Castiglione F, Hedlund P, Weyne E, et al. Intratunical Injection of Human Adipose Tissue–Derived Stem Cells Restores Collagen III/I Ratio in a Rat Model of Chronic Peyronie's Disease. Sex Med 2018;XX:XX–XX.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Oto-rhino-laryngologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Otorhinolaryngology (hsv//eng)
Nyckelord
- Adipose Stem Cell
- Fibrosis
- Peyronie's Disease
- Stem Cell
- Peyronies Disease; Stem Cell; Adipose Stem Cell; Fibrosis
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas
- Av författaren/redakt...
-
Castiglione, Fab ...
-
Hedlund, Petter
-
Weyne, Emanuel
-
Hakim, Lukman
-
Montorsi, France ...
-
Bivalacqua, Trin ...
-
visa fler...
-
De Ridder, Dirk
-
Milenkovic, Uros
-
Ralph, David
-
Garaffa, Giulio
-
Muneer, Asif
-
Joniau, Steven
-
Albersen, Maarte ...
-
visa färre...
- Om ämnet
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Reproduktionsmed ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Medicinska och f ...
-
och Cell och molekyl ...
-
- MEDICIN OCH HÄLSOVETENSKAP
-
MEDICIN OCH HÄLS ...
-
och Klinisk medicin
-
och Oto rhino laryng ...
- Artiklar i publikationen
-
Sexual Medicine
- Av lärosätet
-
Lunds universitet
-
Linköpings universitet