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Transcriptional pro...
Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism
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- Jonsson, Alexander (author)
- Uppsala universitet,Klinisk immunologi
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- Hedin, Anders (author)
- Uppsala universitet,Klinisk immunologi
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- Müller, Malin (author)
- Uppsala universitet,Klinisk immunologi
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- Skog, Oskar, Docent, PhD, 1981- (author)
- Uppsala universitet,Klinisk immunologi
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- Korsgren, Olle (author)
- Uppsala universitet,Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine,Klinisk immunologi,Univ Gothenburg, Inst Med, Gothenburg, Sweden.
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(creator_code:org_t)
- 2020-12-18
- 2020
- English.
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In: Scientific Reports. - BERLIN GERMANY : Springer Science and Business Media LLC. - 2045-2322. ; 10:1
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Abstract
Subject headings
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- In experimental studies, pancreatic islet microvasculature is essential for islet endocrine function and mass, and islet vascular morphology is altered in diabetic subjects. Even so, almost no information is available concerning human islet microvascular endothelial cell (MVEC) physiology and gene expression. In this study, islets and exocrine pancreatic tissue were acquired from organ donors with normoglycemia or impaired glucose metabolism (IGM) immediately after islet isolation. Following single-cell dissociation, primary islet- and exocrine MVECs were obtained through fluorescence-activated cell sorting (FACS) and transcriptional profiles were generated using AmpliSeq. Multiple gene sets involved in general vascular development and extracellular matrix remodeling were enriched in islet MVEC. In exocrine MVEC samples, multiple enriched gene sets that relate to biosynthesis and biomolecule catabolism were found. No statistically significant enrichment was found in gene sets related to autophagy or endoplasmic reticulum (ER) stress. Although ample differences were found between islet- and exocrine tissue endothelial cells, no differences could be observed between normoglycemic donors and donors with IGM at gene or gene set level. Our data is consistent with active angiogenesis and vascular remodeling in human islets and support the notion of ongoing endocrine pancreas tissue repair and regeneration even in the adult human.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- differential expression analysis
- pancreatic-islets
- gene-expression
- blood-flow
- in-vitro
- adult
- phosphorylation
- vascularization
- proliferation
- bioconductor
- Science & Technology - Other Topics
- DIFFERENTIAL EXPRESSION ANALYSIS
Publication and Content Type
- ref (subject category)
- art (subject category)
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