Ancestral sequence reconstruction / edited by David A. Liberles.

• I Introductory scientific overview -- CHAPTER 1 The early days of paleogenetics: connecting molecules to the planet / Steven A. Benner -- CHAPTER 2 Ancestral sequence reconstruction as a tool to understand natural history and guide synthetic biology: realizing and extending the vision of Zuckerkandl and Pauling / Eric A. Gaucher -- CHAPTER 3 Linking sequence to function in drug design with ancestral sequence reconstruction / Janos T. Kodra, Marie Skovgaard, Dennis Madsen, and David A. Liberles -- II Computational methodology and concerns -- CHAPTER 4 Probabilistic models and their impact on the accuracy of reconstructed ancestral protein sequences / Tal Pupko, Adi Doron-Faigenboim, David A. Liberles, and Gina M. Cannarozzi -- CHAPTER 5 Probabilistic ancestral sequences based on the Markovian model of evolution: algorithms and applications / mGina M. Cannarozzi, Adrian Schneider, and Gaston H. Gonnet -- CHAPTER 6 Estimating the history of mutations on a phylogeny / Jonathan P. Bollback, Paul P. Gardner, and Rasmus Nielsen -- CHAPTER 7 Coarse projections of the protein-mutational fitness landscape / F. Nicholas Braun -- CHAPTER 8 Dealing with uncertainty in ancestral sequence reconstruction: sampling from the posterior distribution / David D. Pollock, and Belinda S.W. Chang -- CHAPTER 9 Evolutionary properties of sequences and ancestral state reconstruction / Lesley J. Collins, and Peter J. Lockhart -- CHAPTER 10 Reconstructing the ancestral eukaryote: lessons from the past / Mary J. O'Connell, and James O. McInerney. 
• III Computational applications of ancestral sequence reconstruction -- CHAPTER 11 Using ancestral sequence inference to determine the trend of functional divergence after gene duplication / Xun Gu, Ying Zheng, Yong Huang, and Dongping Xu -- CHAPTER 12 Reconstruction of ancestral proteomes / Toni Gabaldón, and Martijn A. Huynen -- CHAPTER 13 Computational reconstruction of ancestral genomic regions from evolutionarily conserved gene clusters / Etienne G.J. Danchin, Eric A. Gaucher, and Pierre Pontarotti -- IV Experimental methodology and concerns -- CHAPTER 14 Experimental resurrection of ancient biomolecules: gene synthesis, heterologous protein expression, and functional assays / Eric A. Gaucher -- CHAPTER 15 Dealing with model uncertainty in reconstructing ancestral proteins in the laboratory: examples from archosaur visual pigments and coralfluorescent proteins / Belinda S.W. Chang, Mikhail V. Matz, Steven F. Field, Johannes Müller, and Ilke van Hazel -- V Experimental synthesis of ancestral proteins to test biological hypotheses -- CHAPTER 16 Using ancestral gene resurrection to unravel the evolution of protein function / Joseph W. Thornton, and Jamie T. Bridgham -- CHAPTER 17 A thermophilic last universal ancestor inferred from its estimated amino acid composition / Dawn J. Brooks, and Eric A. Gaucher -- CHAPTER 18 The resurrection of ribonucleases from mammals: from ecology to medicine / Slim O. Sassi, and Steven A. Benner -- CHAPTER 19 Evolution of specificity and diversity / Denis C. Shields, Catriona R. Johnston, Iain M. Wallace, and Richard J. Edwards -- Conclusion and a way forward / David A. Liberles. 

• Ancestral sequence reconstruction is a technique of growing importance in molecular evolutionary biology and comparative genomics. As a powerful tool for testing evolutionary and ecological hypotheses, as well as uncovering the link between sequence and molecular phenotype, there are potential applications in almost all fields of applied molecular biology. This book starts with a historical overview of the field, before discussing the potential applications in drug discovery and the pharmaceutical industry. This is followed by a section on computational methodology, which provides a detailed discussion of the available methods for reconstructing ancestral sequences (including their advantages, disadvantages, and potential pitfalls). Purely computational applications of the technique are then covered, including whole proteome reconstruction. Further chapters provide a detailed discussion on taking computationally reconstructed sequences and synthesizing them in the laboratory. The book concludes with a description of the scientific questions where experimental ancestral sequence reconstruction has been utilized to provide insights and inform future research.

Ämnesord

Molekylärbiologi  (sao)

Nucleotide sequence  (LCSH)

Evolutionary genetics  (LCSH)

Molecular biology  (LCSH)

Nyckelord

Ue.049 Biologi: molekylärbiologi

Ue.049 Biologi: molekylärbiologi

Publikations- och innehållstyp

QP625.N89 (LCC)

572.838 (DDC)

Ue.049/DR (kssb/8)

Ue.049 (kssb/8)