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Analysis of nuclear...
Analysis of nuclear protein complexes comprising a-actinin-4 by 2D-electrophoresis and mass-spectrometry
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- Khotin, M.G. (författare)
- Institute of Cytology RAS, St. Petersburg, Russian Federation
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- Turoverova, L.V. (författare)
- Institute of Cytology RAS, St. Petersburg, Russian Federation
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- Podolskaya, E.P. (författare)
- Institute for Analytical Instrumentation RAS, St. Petersburg, Russian Federation
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- Krasnov, I.A. (författare)
- Institute for Analytical Instrumentation RAS, St. Petersburg, Russian Federation
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- Solovyeva, A.V. (författare)
- Institute of Cytology RAS, St. Petersburg, Russian Federation
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- Aksenova, V.Yu. (författare)
- Institute of Cytology RAS, St. Petersburg, Russian Federation
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- Magnusson, Karl-Eric (författare)
- Linköpings universitet,Medicinsk mikrobiologi,Hälsouniversitetet
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- Pinaev, G.P. (författare)
- Institute of Cytology RAS, St. Petersburg, Russian Federation
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- Tentler, D.G. (författare)
- Institute of Cytology RAS, St. Petersburg, Russian Federation
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Institute of Cytology RAS, St Petersburg, Russian Federation Institute for Analytical Instrumentation RAS, St. Petersburg, Russian Federation (creator_code:org_t)
- SP MAIK Nauka/Interperiodica, 2009
- 2009
- Engelska.
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Ingår i: Tsitologiya. - : SP MAIK Nauka/Interperiodica. - 0041-3771. ; 51:8, s. 684-690
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Actin-binding protein a-actinin-4 is a member of spectrin super family. It is located in the cytoplasm and in the nucleus. However, nuclear functions of a-actinin-4 are still not clear. In this study, we analyzed composition of nuclear protein complexes associated with a-actinin-4 in A431 cells. Using 2D electrophoresis, we have determined that about 50 different proteins may be associated with nuclear a-actinin-4. Using mass-spectrometry, we analyzed major proteins of these complexes. ß-Actin, a- and ß-tubulins, ribonucleoprotein A2/B1, which regulates splicing and is associated with ß-actin, peroxiredoxin-1, which is involved in oxidative stress, and glycolytic enzyme D-3-phosphoglycerate dehydrogenase were identified by MALDI-TOF. Detection of these proteins in nuclear complexes with a-actinin-4 may suggest that a-actinin-4 is involved in transcription and splicing. Presence of a-actin in the investigated complexes was confirmed by tandem mass-spectrometry (MALDITOF-TOF). Immunoprecipitation of nuclear proteins with antibodies against a-tubulin confirmed association of a-actinin-4 with a-tubulin in the protein complex. Nuclear a-actinin-4 constitutes of 105 KDa fullsize isoform and two truncated isoforms of 65 and 75 kDa, whereas only the truncated isoform have been found in nuclear complexes with a-tubulin. These data suggest that a-actinin-4 is associated with a number of different nuclear protein complexes which may carry out different functions in the cell nucleus.
Nyckelord
- a-actinin-4; Mass-spectrometry
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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