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Single-cell RNAseq ...
Single-cell RNAseq identifies clonally expanded antigen-specific T-cells following intradermal injection of gold nanoparticles loaded with diabetes autoantigen in humans
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- Hanna, Stephanie J. (författare)
- Cardiff Univ, Wales
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- Thayer, Terri C. (författare)
- Cardiff Univ, Wales; Roberts Wesleyan Univ, NY USA
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- Robinson, Emma J. S. (författare)
- Cardiff Univ, Wales
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- Vinh, Ngoc-Nga (författare)
- Cardiff Univ, Wales
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- Williams, Nigel (författare)
- Cardiff Univ, Wales
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- Landry, Laurie G. (författare)
- Univ Colorado, CO USA
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- Andrews, Robert (författare)
- Cardiff Univ, Wales
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- Siah, Qi Zhuang (författare)
- Oxford Univ Hosp NHS Trust, England
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- Leete, Pia (författare)
- Univ Exeter, England
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- Wyatt, Rebecca (författare)
- Univ Exeter, England
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- Mcateer, Martina A. (författare)
- Univ Oxford, England
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- Nakayama, Maki (författare)
- Univ Colorado, CO USA
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- Wong, F. Susan (författare)
- Cardiff Univ, Wales
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- Yang, Jennie H. M. (författare)
- Guys Hosp, England
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- Tree, Timothy I. M. (författare)
- Guys Hosp, England
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- Ludvigsson, Johnny (författare)
- Linköpings universitet,Avdelningen för barns och kvinnors hälsa,Medicinska fakulteten,Region Östergötland, H.K.H. Kronprinsessan Victorias barn- och ungdomssjukhus
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- Dayan, Colin M. (författare)
- Cardiff Univ, Wales
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- Tatovic, Danijela (författare)
- Cardiff Univ, Wales
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(creator_code:org_t)
- FRONTIERS MEDIA SA, 2023
- 2023
- Engelska.
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Ingår i: Frontiers in Immunology. - : FRONTIERS MEDIA SA. - 1664-3224. ; 14
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Gold nanoparticles (GNPs) have been used in the development of novel therapies as a way of delivery of both stimulatory and tolerogenic peptide cargoes. Here we report that intradermal injection of GNPs loaded with the proinsulin peptide C19-A3, in patients with type 1 diabetes, results in recruitment and retention of immune cells in the skin. These include large numbers of clonally expanded T-cells sharing the same paired T-cell receptors (TCRs) with activated phenotypes, half of which, when the TCRs were re-expressed in a cell-based system, were confirmed to be specific for either GNP or proinsulin. All the identified gold-specific clones were CD8+, whilst proinsulin-specific clones were both CD8+ and CD4+. Proinsulin-specific CD8+ clones had a distinctive cytotoxic phenotype with overexpression of granulysin (GNLY) and KIR receptors. Clonally expanded antigen-specific T cells remained in situ for months to years, with a spectrum of tissue resident memory and effector memory phenotypes. As the T-cell response is divided between targeting the gold core and the antigenic cargo, this offers a route to improving resident memory T-cells formation in response to vaccines. In addition, our scRNAseq data indicate that focusing on clonally expanded skin infiltrating T-cells recruited to intradermally injected antigen is a highly efficient method to enrich and identify antigen-specific cells. This approach has the potential to be used to monitor the intradermal delivery of antigens and nanoparticles for immune modulation in humans.
Ämnesord
- NATURVETENSKAP -- Biologi -- Immunologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Immunology (hsv//eng)
Nyckelord
- nanoparticles; proinsulin peptide; type 1 diabetes; immunomodulation; scRNAseq
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Hanna, Stephanie ...
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Thayer, Terri C.
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Robinson, Emma J ...
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Vinh, Ngoc-Nga
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Williams, Nigel
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Landry, Laurie G ...
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visa fler...
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Andrews, Robert
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Siah, Qi Zhuang
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Leete, Pia
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Wyatt, Rebecca
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Mcateer, Martina ...
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Nakayama, Maki
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Wong, F. Susan
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Yang, Jennie H. ...
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Tree, Timothy I. ...
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Ludvigsson, John ...
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Dayan, Colin M.
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Tatovic, Danijel ...
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visa färre...
- Om ämnet
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- NATURVETENSKAP
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NATURVETENSKAP
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och Biologi
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och Immunologi
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Frontiers in Imm ...
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Linköpings universitet