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p27Kip1 Deficiency ...
p27Kip1 Deficiency Impairs Brown Adipose Tissue Function Favouring Fat Accumulation in Mice
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- Colon-Mesa, Ignacio (författare)
- University of Navarra
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- Sainz, Neira (författare)
- University of Navarra
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- Corrales, Patricia (författare)
- Rey Juan Carlos University
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- Collantes, María (författare)
- University of Navarra
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- Kaldis, Philipp (författare)
- Lund University,Lunds universitet,Institutionen för kliniska vetenskaper, Malmö,Medicinska fakulteten,Metabolism och leversjukdomar,Forskargrupper vid Lunds universitet,Department of Clinical Sciences, Malmö,Faculty of Medicine,Metabolic disorders and liver disease,Lund University Research Groups
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- Martinez, José Alfredo (författare)
- University of Navarra
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- Medina-Gómez, Gema (författare)
- Health Research Institute of Navarra (IDISNA),Rey Juan Carlos University
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- Moreno-Aliaga, María Jesús (författare)
- University of Navarra,Health Research Institute of Navarra (IDISNA),CIBER de Fisiopatología Obesidad y Nutricion (CIBEROBN)
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- Escoté, Xavier (författare)
- Eurecat Technology Center of Catalonia,University of Navarra
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(creator_code:org_t)
- 2023-01-31
- 2023
- Engelska 17 s.
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Ingår i: International Journal of Molecular Sciences. - : MDPI AG. - 1661-6596 .- 1422-0067. ; 24:3
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- The aim of this work was to investigate the effect of the whole-body deletion of p27 on the activity of brown adipose tissue and the susceptibility to develop obesity and glucose homeostasis disturbances in mice, especially when subjected to a high fat diet. p27 knockout (p27−/−) and wild type (WT) mice were fed a normal chow diet or a high fat diet (HFD) for 10-weeks. Body weight and composition were assessed. Insulin and glucose tolerance tests and indirect calorimetry assays were performed. Histological analysis of interscapular BAT (iBAT) was carried out, and expression of key genes/proteins involved in BAT function were characterized by qPCR and Western blot. iBAT activity was estimated by 18F-fluorodeoxyglucose (18FDG) uptake with microPET. p27−/− mice were more prone to develop obesity and insulin resistance, exhibiting increased size of all fat depots. p27−/− mice displayed a higher respiratory exchange ratio. iBAT presented larger adipocytes in p27−/− HFD mice, accompanied by downregulation of both Glut1 and uncoupling protein 1 (UCP1) in parallel with defective insulin signalling. Moreover, p27−/− HFD mice exhibited impaired response to cold exposure, characterized by a reduced iBAT 18FDG uptake and difficulty to maintain body temperature when exposed to cold compared to WT HFD mice, suggesting reduced thermogenic capacity. These data suggest that p27 could play a role in BAT activation and in the susceptibility to develop obesity and insulin resistance.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Nyckelord
- brown adipose tissue
- insulin resistance
- obesity
- p27
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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