Evaluation of p16 and Id1 status and endogenous reference genes in human chondrosarcoma by real-time PCR.

• Both the tumour suppressor, p16, and the helix-loop-helix transcription factor, Id1, have been assigned roles in tumour growth in general and appear to be involved in chondrosarcoma. Id1 has further been found to repress the expression of p16. Therefore, the mRNA expression of these two genes was studied by real-time PCR in a search for prognostic markers in human chondrosarcoma. To get reliable quantitative data, however, the choice of endogenous reference gene for use in the assay is important. Therefore, eleven different endogenous reference genes were evaluated in chondrosarcoma cells and articular chondrocytes. 18S rRNA appeared to be the best choice to use as endogenous reference gene, since it was suitable for both kinds of cells. Several of the other reference genes tested showed variation between individuals or between normal chondrocytes and chondrosarcoma cells. This demonstrates the importance of using a correct endogenous reference gene to get reliable results from quantitative measurements. Both p16 and Id1 showed varied gene expression patterns among the samples and none of these genes could be significantly correlated to prognosis.

Ämnesord

MEDICIN OCH HÄLSOVETENSKAP  -- Klinisk medicin -- Kirurgi (hsv//swe)

MEDICAL AND HEALTH SCIENCES  -- Clinical Medicine -- Surgery (hsv//eng)

Nyckelord

Bone Neoplasms

genetics

pathology

Chondrocytes

metabolism

Chondrosarcoma

genetics

pathology

Cyclin-Dependent Kinase Inhibitor p16

genetics

Gene Expression Profiling

Gene Expression Regulation

Neoplastic

Humans

Inhibitor of Differentiation Protein 1

genetics

Prognosis

RNA

Messenger

genetics

metabolism

RNA

Ribosomal

18S

genetics

Reverse Transcriptase Polymerase Chain Reaction

methods

Publikations- och innehållstyp

ref (ämneskategori)

art (ämneskategori)