Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME

↓ Direkt till sidans innehåll
↓ Direkt till sidans sekundära innehåll (sidomenyn)

Tyck till om SwePub Sök här!

Search: WFRF:(Stieger Stefan) > Recent advances in ...

Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME

Godoy, Patricio (author)

Hewitt, Nicola J. (author)

Albrecht, Ute (author)

Andersen, Melvin E. (author)

Ansari, Nariman (author)

Bhattacharya, Sudin (author)

Bode, Johannes Georg (author)

Bolleyn, Jennifer (author)

Borner, Christoph (author)

Boettger, Jan (author)

Braeuning, Albert (author)

Budinsky, Robert A. (author)

Burkhardt, Britta (author)

Cameron, Neil R. (author)

Camussi, Giovanni (author)

Cho, Chong-Su (author)

Choi, Yun-Jaie (author)

Rowlands, J. Craig (author)

Dahmen, Uta (author)

Damm, Georg (author)

Dirsch, Olaf (author)

Teresa Donato, Maria (author)

Dong, Jian (author)

Dooley, Steven (author)

Drasdo, Dirk (author)

Eakins, Rowena (author)

Ferreira, Karine Sa (author)

Fonsato, Valentina (author)

Fraczek, Joanna (author)

Gebhardt, Rolf (author)

Gibson, Andrew (author)

Glanemann, Matthias (author)

Goldring, Chris E. P. (author)

Jose Gomez-Lechon, Maria (author)

Groothuis, Geny M. M. (author)

Gustavsson, Lena (author): Lund University,Lunds universitet,Institutionen för translationell medicin,Medicinska fakulteten,Department of Translational Medicine,Faculty of Medicine

Guyot, Christelle (author)

Hallifax, David (author)

Hammad, Seddik (author)

Hayward, Adam (author)

Haeussinger, Dieter (author)

Hellerbrand, Claus (author)

Hewitt, Philip (author)

Hoehme, Stefan (author)

Holzhuetter, Hermann-Georg (author)

Houston, J. Brian (author)

Hrach, Jens (author)

Ito, Kiyomi (author)

Jaeschke, Hartmut (author)

Keitel, Verena (author)

Kelm, Jens M. (author)

Park, B. Kevin (author)

Kordes, Claus (author)

Kullak-Ublick, Gerd A. (author)

LeCluyse, Edward L. (author)

Lu, Peng (author)

Luebke-Wheeler, Jennifer (author)

Lutz, Anna (author)

Maltman, Daniel J. (author)

Matz-Soja, Madlen (author)

McMullen, Patrick (author)

Merfort, Irmgard (author)

Messner, Simon (author)

Meyer, Christoph (author)

Mwinyi, Jessica (author)

Naisbitt, Dean J. (author)

Nussler, Andreas K. (author)

Olinga, Peter (author)

Pampaloni, Francesco (author)

Pi, Jingbo (author)

Pluta, Linda (author)

Przyborski, Stefan A. (author)

Ramachandran, Anup (author)

Rogiers, Vera (author)

Rowe, Cliff (author)

Schelcher, Celine (author)

Schmich, Kathrin (author)

Schwarz, Michael (author)

Singh, Bijay (author)

Stelzer, Ernst H. K. (author)

Stieger, Bruno (author)

Stoeber, Regina (author)

Sugiyama, Yuichi (author)

Tetta, Ciro (author)

Thasler, Wolfgang E. (author)

Vanhaecke, Tamara (author)

Vinken, Mathieu (author)

Weiss, Thomas S. (author)

Widera, Agata (author)

Woods, Courtney G. (author)

Xu, Jinghai James (author)

Yarborough, Kathy M. (author)

Hengstler, Jan G. (author)

show less...

(creator_code:org_t)

2013-08-23
2013
English.
In: Archives of Toxicology. - : Springer Science and Business Media LLC. - 0340-5761 .- 1432-0738. ; 87:8, s. 1315-1530

Related links:: https://portal.resea... (primary) (free); show more...; http://dx.doi.org/10... (free); https://link.springe...; https://lup.lub.lu.s...; https://doi.org/10.1...; show less...

Research review (peer-reviewed)

Abstract Subject headings

This review encompasses the most important advances in liver functions and hepatotoxicity and analyzes which mechanisms can be studied in vitro. In a complex architecture of nested, zonated lobules, the liver consists of approximately 80 % hepatocytes and 20 % non-parenchymal cells, the latter being involved in a secondary phase that may dramatically aggravate the initial damage. Hepatotoxicity, as well as hepatic metabolism, is controlled by a set of nuclear receptors (including PXR, CAR, HNF-4 alpha, FXR, LXR, SHP, VDR and PPAR) and signaling pathways. When isolating liver cells, some pathways are activated, e.g., the RAS/MEK/ERK pathway, whereas others are silenced (e.g. HNF-4 alpha), resulting in up- and downregulation of hundreds of genes. An understanding of these changes is crucial for a correct interpretation of in vitro data. The possibilities and limitations of the most useful liver in vitro systems are summarized, including three-dimensional culture techniques, co-cultures with non-parenchymal cells, hepatospheres, precision cut liver slices and the isolated perfused liver. Also discussed is how closely hepatoma, stem cell and iPS cell-derived hepatocyte-like-cells resemble real hepatocytes. Finally, a summary is given of the state of the art of liver in vitro and mathematical modeling systems that are currently used in the pharmaceutical industry with an emphasis on drug metabolism, prediction of clearance, drug interaction, transporter studies and hepatotoxicity. One key message is that despite our enthusiasm for in vitro systems, we must never lose sight of the in vivo situation. Although hepatocytes have been isolated for decades, the hunt for relevant alternative systems has only just begun.

Find in a library

Archives of Toxicology (Search for host publication in LIBRIS)

To the university's database

Find more in SwePub

By the author/editor: Godoy, Patricio; Hewitt, Nicola J ...; Albrecht, Ute; Andersen, Melvin ...; Ansari, Nariman; Bhattacharya, Su ...; show more...; Bode, Johannes G ...; Bolleyn, Jennife ...; Borner, Christop ...; Boettger, Jan; Braeuning, Alber ...; Budinsky, Robert ...; Burkhardt, Britt ...; Cameron, Neil R.; Camussi, Giovann ...; Cho, Chong-Su; Choi, Yun-Jaie; Rowlands, J. Cra ...; Dahmen, Uta; Damm, Georg; Dirsch, Olaf; Teresa Donato, M ...; Dong, Jian; Dooley, Steven; Drasdo, Dirk; Eakins, Rowena; Ferreira, Karine ...; Fonsato, Valenti ...; Fraczek, Joanna; Gebhardt, Rolf; Gibson, Andrew; Glanemann, Matth ...; Goldring, Chris ...; Jose Gomez-Lecho ...; Groothuis, Geny ...; Gustavsson, Lena; Guyot, Christell ...; Hallifax, David; Hammad, Seddik; Hayward, Adam; Haeussinger, Die ...; Hellerbrand, Cla ...; Hewitt, Philip; Hoehme, Stefan; Holzhuetter, Her ...; Houston, J. Bria ...; Hrach, Jens; Ito, Kiyomi; Jaeschke, Hartmu ...; Keitel, Verena; Kelm, Jens M.; Park, B. Kevin; Kordes, Claus; Kullak-Ublick, G ...; LeCluyse, Edward ...; Lu, Peng; Luebke-Wheeler, ...; Lutz, Anna; Maltman, Daniel ...; Matz-Soja, Madle ...; McMullen, Patric ...; Merfort, Irmgard; Messner, Simon; Meyer, Christoph; Mwinyi, Jessica; Naisbitt, Dean J ...; Nussler, Andreas ...; Olinga, Peter; Pampaloni, Franc ...; Pi, Jingbo; Pluta, Linda; Przyborski, Stef ...; Ramachandran, An ...; Rogiers, Vera; Rowe, Cliff; Schelcher, Celin ...; Schmich, Kathrin; Schwarz, Michael; Singh, Bijay; Stelzer, Ernst H ...; Stieger, Bruno; Stoeber, Regina; Sugiyama, Yuichi; Tetta, Ciro; Thasler, Wolfgan ...; Vanhaecke, Tamar ...; Vinken, Mathieu; Weiss, Thomas S.; Widera, Agata; Woods, Courtney ...; Xu, Jinghai Jame ...; Yarborough, Kath ...; Hengstler, Jan G ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmacology and ...

Articles in the publication: Archives of Toxi ...

By the university: Lund University

Search outside SwePub

Extend your search to:: Google; Google Book Search; Google Scholar

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

LIBRIS.kb.se

Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME

Subject headings

Keyword

Publication and Content Type

Find in a library

To the university's database

Find more in SwePub

Search outside SwePub