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Sökning: onr:"swepub:oai:DiVA.org:oru-104579" > Performance of a Sy...

  • Göransson, J.Q-linea AB, Uppsala, Sweden,Univ Copenhagen, Hvidovre Hosp, Dept Clin Microbiol, Hvidovre, Denmark (författare)

Performance of a System for Rapid Phenotypic Antimicrobial Susceptibility Testing of Gram-Negative Bacteria Directly from Positive Blood Culture Bottles

  • Artikel/kapitelEngelska2023

Förlag, utgivningsår, omfång ...

  • American Society for Microbiology,2023
  • printrdacarrier

Nummerbeteckningar

  • LIBRIS-ID:oai:DiVA.org:oru-104579
  • https://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-104579URI
  • https://doi.org/10.1128/jcm.01525-22DOI
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-511331URI

Kompletterande språkuppgifter

  • Språk:engelska
  • Sammanfattning på:engelska

Ingår i deldatabas

Klassifikation

  • Ämneskategori:ref swepub-contenttype
  • Ämneskategori:art swepub-publicationtype

Anmärkningar

  • The rapid administration of optimal antimicrobial treatment is paramount for the treatment of bloodstream infections (BSIs), and rapid antimicrobial susceptibility testing (AST) results are essential. Q-linea has developed the ASTar system, a rapid phenotypic AST device. Here, we report the performance of the ASTar BC G- (Gram-negative) kit when assessed according to the ISO 20776-2:2007 standard for performance evaluation of in vitro diagnostic AST devices. The evaluated ASTar BC G- kit uses a broad panel of 23 antimicrobials for the treatment of BSIs caused by Gram-negative fastidious and nonfastidious bacteria across a range of 6 to 14 2-fold dilutions, including cefoxitin as a screening agent for AmpC-producing Enterobacterales. The ASTar system processes blood culture samples to generate data on MICs and susceptible, intermediate, or resistant (SIR) category. The automated protocol includes concentration determination and concentration adjustment to enable a controlled inoculum, followed by broth microdilution (BMD) and microscopy performed continuously to generate MIC values within approximately 6 h once the test is run on the ASTar system. The performance of the ASTar system was assessed against the ISO 20776-2:2007 standard BMD reference method. Testing was performed across three sites, with results from 412 contrived blood cultures and 74 fresh clinical blood cultures. The ASTar system was also tested for reproducibility, with triplicate testing of 11 strains. The accuracy study comprised 8,650 data points of bacterium-antimicrobial tests. The ASTar system demonstrated an overall essential agreement (EA) of 95.8% (8,283/8,650) and a categorical agreement (CA) of 97.6% (8,433/8,639) compared to the reference BMD method. The overall rate of major discrepancies (MDs) was 0.9% (62/6,845), and that of very major discrepancies (VMDs) was 2.4% (30/1,239). This study shows that the ASTar system delivers reproducible results with overall EA and CA of >95%.

Ämnesord och genrebeteckningar

Biuppslag (personer, institutioner, konferenser, titlar ...)

  • Sundqvist, Martin,1974-Örebro universitet,Institutionen för medicinska vetenskaper,Region Örebro län,Department of Laboratory Medicine, Clinical Microbiology,Örebro Univ, Fac Med & Hlth, Dept Lab Med, Clin Microbiol, Örebro, Sweden(Swepub:oru)mist (författare)
  • Ghaderi, E.Department of Bacteriology, Uppsala University Hospital, Uppsala, Sweden,Uppsala Univ Hosp, Dept Bacteriol, Uppsala, Sweden (författare)
  • Lisby, J. G.Department of Clinical Microbiology, University of Copenhagen, Hvidovre Hospital, Hvidovre, Denmark (författare)
  • Molin, Y.Q-linea AB, Uppsala, Sweden (författare)
  • Eriksson, E.Q-linea AB, Uppsala, Sweden (författare)
  • Carlsson, S.Q-linea AB, Uppsala, Sweden (författare)
  • Cederlöf, A.Q-linea AB, Uppsala, Sweden (författare)
  • Ellis, L.Q-linea AB, Uppsala, Sweden (författare)
  • Melin, J.Q-linea AB, Uppsala, Sweden (författare)
  • Q-linea AB, Uppsala, SwedenUniv Copenhagen, Hvidovre Hosp, Dept Clin Microbiol, Hvidovre, Denmark (creator_code:org_t)

Sammanhörande titlar

  • Ingår i:Journal of Clinical Microbiology: American Society for Microbiology61:30095-11371098-660X

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