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Sökning: onr:"swepub:oai:DiVA.org:ri-26749" > Adsorption of compl...

LIBRIS Formathandbok  (Information om MARC21)
FältnamnIndikatorerMetadata
00002459naa a2200397 4500
001oai:DiVA.org:ri-26749
003SwePub
008161208s1996 | |||||||||||000 ||eng|
024a https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-267492 URI
040 a (SwePub)ri
041 a engb eng
042 9 SwePub
072 7a ref2 swepub-contenttype
072 7a art2 swepub-publicationtype
100a Malmsten, Mu RISE,YKI – Ytkemiska institutet4 aut
2451 0a Adsorption of complement protein C3 at polymer surfaces
264 1c 1996
338 a print2 rdacarrier
500 a A1038
520 a The adsorption of C3 at poly(methyl methacrylate) (PMMA) and poly(styrene) (PS) surfaces was investigated with in situ ellipsometry and compared to that at (hydrophilic and negatively charged) silica and (hydrophobic) methylated silica. The adsorption of C3 at PMMA was higher than that at PS, while the adsorbed layer thickness was the same for the two surfaces. For both PMMA and PS the adsorbed layer thickness (10±2 nm) corresponds rather closely to that of end-on oriented C3 molecules. The adsorption of C3 at PMMA and PS was found to be intermediate between that at silica and methylated silica, although the adsorbed layer thickness was similar for all surfaces. The competitive adsorption between C3, human serum albumin (HSA), and factor B was investigated with ellipsometry and total internal reflection fluorescence spectroscopy (TIRF). Addition of HSA after C3 preadsorption resulted in fractional C3 desorption for both PMMA and PS. Factor B deposition at PS after preadsorption of C3 and blocking with HSA was found to be largely due to specific binding to C3/C3b, while in the case of PMMA, factor B was largely accumulated through passive (displacement) adsorption.
653 a Adsorption
653 a C3
653 a complement
653 a complement activation
653 a ellipsometry
653 a polymer
653 a poly(methyl methacrylate)
653 a poly(styrene)
700a Lassen, Bou RISE,Kemiska processer och läkemedel4 aut
700a Westin, J4 aut
700a Gölander, C-G4 aut
700a Larsson, R4 aut
700a Nilsson, UR4 aut
710a RISEb YKI – Ytkemiska institutet4 org
773t Journal of Colloid and Interface Scienceg 179, s. 163-172q 179<163-172x 0021-9797x 1095-7103
8564 8u https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-26749

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Malmsten, M
Lassen, Bo
Westin, J
Gölander, C-G
Larsson, R
Nilsson, UR
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Journal of Collo ...
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