Neither Notch1 expression nor cellular size correlate with mesenchymal stem cell properties of adult articular chondrocytes.

Karlsson, Camilla,1977Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg (författare)

BACKGROUND: Tissue repair is thought to be regulated by progenitor cells, which in other tissues are characterized by their Notch1 expression or small cellular size. Here we studied if these traits affect the chondrogenic potential and are markers for multipotent progenitor cell populations in adult articular cartilage. METHODS: Directly isolated articular chondrocytes were sorted with regard to their Notch1 expression or cellular size. Their colony forming efficiency (CFE) and their potential to differentiate towards adipogenic, osteogenic and chondrogenic lineages were investigated. The different sorted populations were also expanded in monolayer and analyzed in the same manner as the directly isolated cells. RESULTS: No differences in CFE or adipogenic, osteogenic and chondrogenic potentials were detected among the sorted populations. Expanded cells displayed a higher osteochondral potential than directly isolated cells. CONCLUSION: Cellular size or Notch1 expression is not per se a specific marker for mesenchymal progenitor cells in adult articular cartilage. Monolayer-expanded adult chondrocytes contain a larger mesenchymal progenitor cell-like population than directly isolated cells, highly likely as a result of dedifferentiation. If there are resident Notch1-positive cells or cells of a specific size in adult articular cartilage with functional features of progenitor cells, the population consists of only a very small number of cells.

NATURVETENSKAP Biologi Molekylärbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Molecular Biology hsv//eng
MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Cell- och molekylärbiologi hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Cell and Molecular Biology hsv//eng
NATURVETENSKAP Biologi Cellbiologi hsv//swe
NATURAL SCIENCES Biological Sciences Cell Biology hsv//eng
Adipogenesis
Adult Stem Cells
cytology
metabolism
Animals
Cartilage
Articular
cytology
metabolism
Cattle
Cell Culture Techniques
Cell Differentiation
Cell Separation
Cell Size
Chondrocytes
cytology
metabolism
Colony-Forming Units Assay
Flow Cytometry
Immunohistochemistry
Mesenchymal Stem Cells
cytology
metabolism
Osteogenesis
Receptor
Notch1
metabolism
Cattle

Stenhamre, HannaGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg,Polymerteknologi,Institutionen för kemi- och bioteknik,Chalmers tekniska högskola,Polymer Technology,Department of Chemical and Biological Engineering,Chalmers University of Technology(Swepub:cth)kb99stha (författare)
Sandstedt, JoakimGothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg(Swepub:gu)xsjoad (författare)
Lindahl, Anders,1954Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine,University of Gothenburg(Swepub:gu)xlandy (författare)
Göteborgs universitetInstitutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin (creator_code:org_t)

Av författaren/redakt...: Karlsson, Camill ...; Stenhamre, Hanna; Sandstedt, Joaki ...; Lindahl, Anders, ...

Om ämnet

MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Medicinska och f ...; och Cell och molekyl ...

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