Mitochondrial Regulation Methods and protocols / edited by Carlos M. Palmeira, Anabela P. Rolo.

• 1 Isolation of Crude Mitochondrial Fraction from Cells / Mariusz R. Wieckowski and Lech Wojtczak -- 2 Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of MicroRNA and Protein Expressions / Pedro M. Borralho, Clifford J. Steer, and Cecília M. P. Rodrigues -- 3 PCR Based Determination of Mitochondrial DNA Copy Number in Multiple Species / John P. Rooney, Ian T. Ryde, Laurie H. Sanders, Evan H. Howlett, Meryl D. Colton, Kaylyn E. Germ, Greg D. Mayer, J. Timothy Greenamyre, and Joel N. Meyer -- 4 NAD+ Content and Its Role in Mitochondria / Wei Li and Anthony A. Sauve -- 5 Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes / Ana P. Gomes and David A. Sinclair -- 6 Measurement of Mitochondrial Oxygen Consumption Rates in Mouse Primary Neurons and Astrocytes / Sofia M. Ribeiro, Alfredo Giménez-Cassina, and Nika N. Danial -- 7 Determination of Oxidative Phosphorylation Complexes Activities / João S. Teodoro, Carlos M. Palmeira, and Anabela P. Rolo -- 8 Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Muscle Biopsies from Patients with Mitochondrial Defects / Agnieszka Karkucinska-Wieckowska, Maciej Pronicki, and Mariusz R. Wieckowski -- 9 A Method Aimed at Assessing the Functional Consequences of the Supramolecular Organization of the Respiratory Electron Transfer Chain by Time- Resolved Studies / Fabrice Rappaport -- 10 BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function / Elena Silvestri, Assunta Lombardi, Federica Cioffi, and Fernando Goglia -- 11 Detection of UCP1 Protein and Measurements of Dependent GDP-Sensitive Proton Leak in Non- phosphorylating Thymus Mitochondria / Kieran J. Clarke, Audrey M. Carroll, Gemma O'Brien, and Richard K. Porter -- 12 Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry / Ignazio Grattagliano, Leonilde Bonfrate, Michele Lorusso, Luigi Castorani, Ornella de Bari, and Piero Portincasa -- 13 Following Mitochondria Dynamism: Confocal Analysis of the Organelle Morphology / Francesca R. Mariotti, Mauro Corrado, and Silvia Campello -- 14 Analysis of Pro-apoptotic Protein Trafficking to and from Mitochondria / Ignacio Vega-Naredo, Teresa Cunha-Oliveira, Teresa L. Serafim, Vilma A. Sardao, and Paulo J. Oliveira -- 15 Mitophagy and Mitochondrial Balance / Simone Patergnani and Paolo Pinton. 

• In this volume expert researchers in the field detail the many methods for further research into regulation of mitochondrial function. Chapters focus on mitochondria with other cellular components, discussing how these interactions influence the dynamics of mitochondrial structure and biogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Ämnesord

Mitochondria  -- Laboratory manuals (LCSH)

Mitochondria  (MeSH)

Publikations- och innehållstyp

571.657 (DDC)

Ue.0393 (kssb/8 (machine generated))