| 1. |
- Dao, Trong Tuan, et al.
(författare)
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Resveratrol suppressed lps-induced cox-2 VIA miR-146a-5p inhibition in raw246.7 cells
- 2017
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Ingår i: Farmacia. - 0014-8237. ; 65:2, s. 214-218
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Tidskriftsartikel (refereegranskat)abstract
- Trans-resveratrol (Res) is a well-known natural stilbene frequently found in grapes which have been reported to possess antioxidant, anti-cancer activities and inhibited COX-2 expression. MicroRNAs (miRNAs) are short endogenous non-coding RNAs involved in the regulation of mRNA stability and protein synthesis. In our research, resveratrol isolated from Vitis heyneana Roem. & Schult Vitis heyneana was observed to suppress lipopolysaccharides (LPS)-induced COX-2 expression in Raw264.7 cells in a dose dependent manner. Using qPCR it was revealed that LPS induced the expression of miR-25, miR- 125a, miR-125b, miR-146a-5p, miR-146a-3p and miR-455. However, we only observed miR-146a-5p expression significantly decreased in resveratrol compared to untreated-control group. In addition, resveratrol abrogated the effect of miR-146a-5p mimic induced-COX-2 expression in Raw264.7 cells. Taken together, this study demonstrated for the first time the involvement of miR-146a-5p in resveratrol inhibited LPS-induced COX-2 expression in Raw264.7 cells.
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| 2. |
- Mihon, Mirela, et al.
(författare)
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INFLUENCE OF THE SEPARATION PARAMETERS APPLIED FOR DETERMINATION OF IMPURITIES FDG AND CLDG
- 2017
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Ingår i: Farmacia. - : SOC STINTE FARMACEUTICE ROMANIA. - 0014-8237 .- 2065-0019. ; 65:1, s. 153-158
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Tidskriftsartikel (refereegranskat)abstract
- 2-fluoro-2-deoxy-D-glucose (FDG) and 2-chloro-2-deoxy-D-glucose (CIDG) are chemical impurities found in the 2-[F-18]fluoro-2-deoxy-D-glucose products (F-18-FDG). The objective of this study was to find the best condition for the separation of FDG and CIDG, evaluating different columns under various operating conditions. Chromatographic parameters such as column temperature, composition and flow rate of the mobile phase were the independent variables used in the optimization process. The optimized method was validated and validation results showed a good accuracy, repeatability and reproducibility.
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| 3. |
- Trifonova, Olga, et al.
(författare)
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Assessment of the granulation process effect on morpho-anatomical features and biologically active substances composition of senna leaves
- 2021
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Ingår i: Farmacia. - : Societatea de Stiinte Farmaceutice din Romania. - 0014-8237 .- 2065-0019. ; 69:1, s. 44-50
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Tidskriftsartikel (refereegranskat)abstract
- Cassia senna L. and Cassia angustifolia Vahl. leaves and their preparations are widely acknowledged herbal drugs. Recent development of a novel dosage form - cut-pressed granules - enhances convenience of use in a home setting. However, manufacturing of cut-pressed granules is feasible only if the quality of the material doesn't decrease during granulation. The aim of the study was to assess possible changes in identification parameters and in composition of biologically active substances in Senna leaves after processing. It was shown that TLC chromatographic profiles of herbal drug remain unchanged. Spectrophotometric assessment confirmed that the anthracene aglycones content of the herbal material was also not affected by granulation. It can be concluded that the quality of cut-pressed granules obtained from Senna leaves is not inferior compared to the raw material.
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