| 1. |
- Fahmy, Khalid, et al.
(författare)
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Expression analysis of a family of developmentally-regulated cytosolic sulfotransferases (SULTs) in Drosophila.
- 2013
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 150:2-3, s. 44-48
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Tidskriftsartikel (refereegranskat)abstract
- We have compared the amino acid sequence of all four cytosolic sulfotransferases (SULTs) in Drosophila and analyzed their spatial expression patterns during development. Three out of four SULTs show distinct expression activity during embryogenesis, while the 4th SULT shows expression only post-embryonically. st1, st3 and st4 are expressed in non-overlapping expression domains mainly confined to organs of the alimentary canal such as esophagus, malphigian tubules, hindgut, as well as in the tracheal system. All these organs are surrounded by the hemolymph suggesting that Drosophila SULTs exert their function in detoxification of substances upon influx from the hemolymph.
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| 2. |
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| 3. |
- Lind-Halldén, Christina, et al.
(författare)
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Genetic variation in Arabidopsis suecica and its parental species A-arenosa and A-thaliana
- 2002
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 136:1, s. 45-50
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Tidskriftsartikel (refereegranskat)abstract
- Random amplified polymorphic DNA (RAPD) markers were used to estimate the level of genetic variation in Swedish accessions of the allopolyploid Arabidopsis suecica and its parental species A. thaliana and A. arenosa. The results showed clear differences among the three species with respect to the level of variation. A. arenosa was highly variable, A. thaliana showed a moderate level of variation whereas A. suecica was much less variable than the two other species. An extended analysis covering 19 Swedish populations of A. suecica corroborated the low level of variation in this species, yet 16 unique phenotypes were observed. No isolation by distance was observed. When the genetic variation was partitioned among and within populations of A. suecica, the results showed that the majority of the variation (81 %) occurred among populations. This result is interpreted as a strong indication that A. suecica is autogamous in nature.
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| 4. |
- Pelger, Susanne, et al.
(författare)
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Evolution of hordein gene organization in three Hordeum species.
- 1993
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 119:3, s. 219-231
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Tidskriftsartikel (refereegranskat)abstract
- The inheritance pattern of hordein, the seed storage protein in barley, has been studied in two wild Hordeum species, H. murinum and H. pusillum. Three different diploid populations of each species were crossed, and the F1 plants were self-pollinated. The seeds with an F2 genotype were studied by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Several differences could be observed in the banding patterns of the parental populations. The analysis of recombinant banding patterns showed that in H. murinum there are at least four segregating loci, of which two code for B- and two for C-hordein. All the loci are linked on the same chromosome. In H. pusillum at least six segregating loci were found, of which three code for B and three for C-hordein. Five of the loci are linked, while the sixth showed independent segregation. The organization of the hordein genes differs not only between these two species but also between them and the different forms of H. vulgare, as well as with other species belonging to the tribe Triticeae. Extensive rearrangements must obviously have taken place among the members of the hordein gene family since the divergence of the species in the Hordeum genus. The possibility is discussed that the genes have been moved through transposition, a possible mechanism for the physical divergence of tandemly repeated sequences.
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| 5. |
- Pelger, Susanne, et al.
(författare)
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The reaction of monoclonal antibodies with hordeins from five different Hordeum species.
- 1989
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 111:3, s. 273-279
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Tidskriftsartikel (refereegranskat)abstract
- Hordeins from H. vulgare, H. murinum, H. pusillum, H. bulbosum, and H. brevisubulatum were probed with five monoclonal antibodies raised against different hordein polypeptides from Hordeum vulgare cv. ‘Bomi’. The response pattern differed widely among species. One of the antibodies, CMpBChorl, was found to react with hordein from every tested species, and all of the antibodies reacted with at least one of the wild barley species. Antibodies reacting with many different hordein polypeptides in ‘Bomi’ showed a wide reaction also with other species, while more specific antibodies gave limited reactions outside of domesticated barley. In some cases hordein polypeptides reacting with a specific antibody differed in electrophoretic mobility between species, indicating that the hordein genes involved have undergone concerted evolution.
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| 6. |
- Säll, Torbjörn, et al.
(författare)
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Mode of reproduction in Arabidopsis suecica
- 2004
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 141:3, s. 313-317
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Tidskriftsartikel (refereegranskat)abstract
- The breeding system of Arabidopsis suecica was investigated through genetic analysis of microsatellite segregation patterns in Five controlled crosses as well as in 16 single-mother families collected in the wild. Analysis of single and two-locus segregations in the F-2 generation following a cross clearly shows that A. suecica is reproduces sexually. The single-mother families show a high level of homozygosity corroborating earlier results indicating a high level of inbreeding. The high level of individual homozygosity is due both to a high level of selfing and to the underlying population structure.
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| 7. |
- Troedsson, U, et al.
(författare)
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Application of antisense transformation of a barley chitinase in studies of arbuscule formation by a mycorrhizal fungus
- 2005
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 142:1, s. 65-72
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Tidskriftsartikel (refereegranskat)abstract
- Barley (Hordeum vulgare L.) plants of two commercial cultivars were transformed with sense and antisense constructs of a chitinase class II gene in order to develop a transformation system for this gene in barley. Transformation of embryos with the two antisense constructs resulted in ten regenerated plants, while no plants could be obtained using the sense construct. The presence of the inserted construct could be confirmed for six of the plants by PCR analysis. This system was used to study the role of class II chitinase in the regulation of mycorrhizal symbiosis. The colonization of two of the antisense transformants by the arbuscular mycorrhizal fungus Glomus intraradices was investigated microscopically and by use of signature fatty acids. The arbuscular incidence increased in transformed barley, and one transformant supported higher extraradical mycelium biomass. It is concluded that antisense transformation of barley could be a useful tool in investigations on the symbiosis between barley and mycorrhizal fungi.
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| 8. |
- Bensch, Staffan, et al.
(författare)
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Partial albinism in a semi-isolated population of great reed warblers
- 2000
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 133:2, s. 167-170
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Tidskriftsartikel (refereegranskat)abstract
- Albinism in birds is thought to result from the expression of recessive alleles that disrupt melanin pigmentation at feather development. We have studied great reed warblers Acrocephalus arundinaceus in a recently founded and increasing population in Sweden during 15 years for the presence of birds with albinistic feathers. The study population was founded in 1978 and the few cases of albinism was exclusively recorded during the first five pears of our study (1985-1989). This fits to the expected pattern if albinism is governed by recessive alleles; we have previously demonstrated that the population suffered from inbreeding during the first years of our study. The albinistic birds experienced a similar lifetime reproductive success as normally coloured birds.
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| 9. |
- von Schantz, Torbjörn, et al.
(författare)
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Mate choice, male condition-dependent ornamentation and MHC in the pheasant
- 1997
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 127:2, s. 133-140
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Tidskriftsartikel (refereegranskat)abstract
- The Hamilton and Zuk model predicts that genes for resistance to various pathogens may be continuously heritable due to selection disequilibria caused by coadaptional cycles of hosts and pathogens. The model further suggests that the expression of male secondary ornaments is condition-dependent and that only individuals with superior genetic disease resistance and vigour can fully express exaggerated secondary ornaments. Female choice is therefore expected to discriminate among males on the basis of secondary sexual characters in order to pass on genes for disease resistance that improve fitness in the offspring. In wild ring-necked pheasants, Phasianus colchicus, of the Revinge area in southern Sweden, females prefer to mate with long-spurred males and data on reproductive success indicate that they may improve their chicks' survival rate by doing so. Male spur length is positively correlated with age, body size and viability. MHC genotyping for both class I and class II B of pheasant males trapped in the study area identified eight MHC haplotypes and a total of 17 different MHC genotypes. Multivariate analyses revealed that MHC genotype is significantly associated with variation in both male spur length and male viability. These data indicate that polymorphic genes with a central role in immune recognition can be associated with viability and the expression of a condition-dependent intersexually selected male trait, thus supporting essential parts of the Hamilton and Zuk model.
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| 10. |
- Andersson, Stefan, et al.
(författare)
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Inbreeding depression in a rare plant, Scabiosa canescens (Dipsacaceae)
- 2002
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Ingår i: Hereditas. - : Springer Science and Business Media LLC. - 1601-5223 .- 0018-0661. ; 136:3, s. 207-211
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Tidskriftsartikel (refereegranskat)abstract
- Plants from a population of Scabiosa canescens, a locally rare species with a narrow ecological amplitude, were raised under uniform growth conditions to examine the phenotypic effects of one generation selfing and outcrossing. Particular attention was given to direct components of fitness (seedling biomass, rosette leaf number, head number, flower number per head), but two morphological characters (plant height, flower size) were also considered. Estimates of inbreeding depression (delta), adjusted for maternal effects and lack of balance, were compared and tested for significance using randomization and bootstrap procedures. Inbreeding significantly depressed several characters during both early and late stages of the life cycle, with delta ranging from 0.14 (flower size) to 0.37 (seedling biomass). Based on these and other results, we propose that S. canescens is susceptible to inbreeding and that the genetic basis of inbreeding depression varies across life stages.
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