| 1. |
- Hellmén, Eva
(författare)
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Macrophage regulation of Wnt pathway in canine and murine mammary cancer cells
- 2023
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Ingår i: Medycyna Weterynaryjna. - : Medycyna Weterynaryjna - Redakcja. - 0025-8628. ; 79, s. 227-231
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Tidskriftsartikel (refereegranskat)abstract
- Interactions between various cell types in a tumor microenvironment (TME) are important for cancer progression and metastasis. Tumor-associated macrophages (TAMs) play a key role in this intratumoral dialogue regulating Wnt signaling pathway in cancer cells. Co-culture of canine and murine tumor cells with TAMs lead to inhibition of the canonical Wnt pathway and activation of the non-canonical Wnt pathway in tumor cells resulting in change in tumor cell phenotype and subsequent higher potential to invasion and metastasis. These molecular changes in cancer cells that are induced by TAMs resemble the quercetin activity. This natural product is a plant flavonoid from the group of polyphenols. Its biological function is inhibition of the canonical Wnt pathway. As quercetin supplementation is broadly discussed in cancer patients, findings of this study show that this compound should be used with caution, as it may enhance cancer spread.
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| 2. |
- Larska, Magdalena
(författare)
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Application of real time RT-PCR for the detection of type 1 and type 3 of bovine viral diarrhea virus (BVDV) in biological material
- 2011
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Ingår i: Medycyna Weterynaryjna. - 0025-8628. ; 67, s. 483-487
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to use real time RT-PCR for the detection of genetic material of bovine viral diarrhea virus (BVDV) type 1 and type 3 in serum and milk samples. Material tested included the fetal calf serum used for cell culture, serum samples from healthy calves and from calves experimentally inoculated with BVDV type 1 and type 3, and milk samples (pasteurized and treated with ultra high temperature). Sensitivity of the test was 200 copies of RNA per reaction (10(5) viral RNA copies per ml) for both types of BVDV, using dedicated primers and standards. Detection limit was 102 tissue culture infectious dose 50 (TCID(50)) and 1 TCID(50) for type 1 and type 3, respectively. Diagnostic specificity of the method was 100%. Out of 10 samples of milk, 3 were positive for BVDV type I, while none was positive for type 3. On the other hand, BVDV type 3 was found in 6 out of 10 samples of fetal calf serum. Real Time RT-PCR for BVDV type 1 and type 3 proved to be a highly sensitive and highly specific technique, enabling the detection of viral genetic material in various samples, even when its detection by virus isolation or antigen ELISA tests is impossible because of virus inactivation by such processes as high temperature, gamma irradiation, or the presence of virus neutralizing antibodies.
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| 3. |
- Sliwa, E, et al.
(författare)
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Influence of dexamethasone and alpha-ketoglutarate administration to pregnant sows on the level of some hormones in the blood plasma of newborn un-suckled piglets
- 2005
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Ingår i: Medycyna Weterynaryjna. - 0025-8628. ; 61:12, s. 1405-1407
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to establish the influence of dexamethasone and alpha-ketoglutarate administration to pregnant sows on the prenatal programming of the growth hormone level, cortisol, insulin and IGF-I in the blood serum of newborn piglets assessed just after their birth and prior to suckling. The foetal development is dependent on and determined by hormones such as glucocorticoids as well as nutrient supply through the sow, especially from the digestive tract. All these factors determine pre- and neonatal growth. The experiment was conducted over the final 24 days of pregnancy in sows treated i.m. with dexamethasone at a dose of 3 mg per sow every second day or alpha-ketoglutarate administered orally daily at a dose of 0.4 g/kg b.w. The blood samples were collected from the subclavian vein of un-suckled piglets immediately following their birth and were centrifuged straight away and the serum stored at -25 degrees C until further analysis. Hormone concentrations were determined using ELISA kit. The obtained results indicate that maternal administration of dexamethasone increased the level of cortisol while alpha-ketoglutarate increased the levels of cortisol and IGF I whereas simultaneous administration of dexamethasone and AKG significantly increased the growth hormone. These results of separate or simultaneous maternal administration of dexamethasone and alpha-ketogluta rate during the last 24 days of pregnancy indicated the activation of different mechanisms and different prenatal effects on the levels of cortisol, growth hormone and IGF I in serum assessed immediately following the birth of the piglets.
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| 4. |
- Sliwa, E, et al.
(författare)
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Influence of dexamethasone and alpha-ketoglutarate on growth and development of the skeletal system of pigs
- 2005
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Ingår i: Medycyna Weterynaryjna. - 0025-8628. ; 61:5, s. 526-531
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the study was to establish the influence of dexamethasone and/or alpha-ketoglutarate (AKG) on the growth, mineralization, geometric and mechanical properties of ribs as a methodological model. The experiment was conducted during the final 24 days of prenatal life and first 14 days of neonatal life. The volumetric bone density of the trabecular and cortical bone was determined separately using the quantitative computed tomography (QCT) method and Somatom AR. T - Siemens apparatus. The mechanical properties were determined using the three-point bending test according to the method by Ferretti et al. The obtained results indicate that maternal administration of dexamethasone increased volumetric mineral density, although the mechanical parameters had lower values. Prenatal administration of AKG decreased all the geometric and mechanical parameters of the investigated ribs. The results obtained during the administration of the compound in neonatal life were the opposite of the prenatal results. Dexamethasone and AKG given together had the same influence on mineralization and other properties AND increasing their values during prenatal and neonatal life. This model of administrating dexamethasone and AKG maternally during prenatal life with the intention of provoking structural growth of the skeleton may be useful in further elucidating some biological and pharmacological factors on bone formation in prenatal life.
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