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- Kurth, Thomas, et al.
(författare)
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Electron Microscopy of the Amphibian Model Systems Xenopus laevis and Ambystoma mexicanum
- 2010
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Ingår i: Methods in Cell Biology. - 0091-679X. ; 96, s. 395-423
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Forskningsöversikt (refereegranskat)abstract
- In this chapter we provide a set of different protocols for the ultrastructural analysis of amphibian (Xenopus, axolotl) tissues, mostly of embryonic origin. For Xenopus these methods include: (1) embedding gastrulae and tailbud embryos into Spurr's resin for TEM, (2) post-embedding labeling of methacrylate (K4M) and cryosections through adult and embryonic epithelia for correlative LM and TEM, and (3) pre-embedding labeling of embryonic tissues with silver-enhanced nanogold. For the axolotl (Ambystoma mexicanum) we present the following methods: (1) SEM of migrating neural crest (NC) cells; (2) SEM and TEM of extracellular matrix (ECM) material; (3) Cryo-SEM of extracellular matrix (ECM) material after cryoimmobilization; and (4) TEM analysis of hyaluronan using high-pressure freezing and HABP labeling. These methods provide exemplary approaches for a variety of questions in the field of amphibian development and regeneration, and focus on cell biological issues that can only be answered with fine structural imaging methods, such as electron microscopy.
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- Carannante, Valentina, et al.
(författare)
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Generation of tumor spheroids in microwells to study NK cell cytotoxicity, infiltration and phenotype
- 2023
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Ingår i: Methods in Cell Biology. - : Elsevier BV. - 0091-679X. ; , s. 195-208
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- The development of new immunotherapeutic drugs and combinatorial strategies requires the implementation of novel methods to test their efficacy in vitro. Here, we present a series of miniaturized in vitro assays to assess immune cell cytotoxic activity, infiltration, and phenotype in renal carcinoma spheroids with the use of a recently developed multichambered microwell chip. We provide protocols for tumor spheroid formation, NK cell culture, fluorescence labelling and imaging of live or fixed cells directly in the chip together with data analysis.
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- Cerrato, G, et al.
(författare)
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Live cell imaging of LC3 dynamics
- 2021
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Ingår i: Methods in cell biology. - : Elsevier. - 0091-679X. ; 164, s. 27-38
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Tidskriftsartikel (refereegranskat)
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- Chen, G, et al.
(författare)
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Monitoring TFEB translocation
- 2021
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Ingår i: Methods in cell biology. - : Elsevier. - 0091-679X. ; 164, s. 1-9
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Tidskriftsartikel (refereegranskat)
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