| 1. |
- Lund, B, et al.
(författare)
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Differential expression of interferon genes in a substrain of Namalwa cells.
- 1985
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Ingår i: Journal of interferon research. - 0197-8357. ; 5:2, s. 229-38
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Tidskriftsartikel (refereegranskat)abstract
- A substrain of Namalwa cells producing a high ratio of beta-interferon (IFN-beta) versus alpha-interferon (IFN-alpha) was investigated by constructing a cDNA library after induction with Sendai virus. The library was screened by two synthetic oligonucleotides, one specific for IFN-alpha and one complementary to both IFN-alpha and IFN-beta. Rescreening the library with two full-length cDNAs encoding IFN-alpha and IFN-beta, respectively, revealed that the frequency of the IFN-alpha and IFN-beta clones reflected the activities of IFN-alpha and IFN-beta obtained by functional assays. On the cDNA level, the dominating species was identical with the type of IFN-alpha A or IFN-alpha 2; however, one new type of cDNA also was found that was similar to the previously described IFN-alpha C. Only one type of cDNA was found encoding IFN-beta, although several IFN-beta proteins have been detected in the analyzed cell line.
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| 2. |
- Sundström, S, et al.
(författare)
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Growth-state independent induction of specific proteins in Swiss 3T3 cells by interferon.
- 1983
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Ingår i: Journal of interferon research. - 0197-8357. ; 3:2, s. 223-9
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Tidskriftsartikel (refereegranskat)abstract
- The early effects of interferon (IFN) on the pattern of protein synthesis in Swiss 3T3 mouse fibroblasts were investigated by two-dimensional gel electrophoresis of 35S-methionine labeled cell extracts. IFN induced within 4 h the synthesis of three proteins with molecular weights of 48, 49, and 50 kD. An increase in the rate of synthesis of a 31 kD protein was also found. These changes were observed after IFN treatment of exponentially growing cells, density-inhibited cells, serum-starved cells, and serum-stimulated quiescent cells. The same protein patterns were obtained from cells treated with IFN-alpha or IFN-beta. The IFN response was blocked by the addition of actinomycin D, implying de novo transcription of the corresponding species of messenger RNA.
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