| 1. |
- Hagman, Ragnvi
(författare)
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Serum alpha-1-acid glycoprotein concentrations in 26 dogs with pyometra
- 2011
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Ingår i: Veterinary Clinical Pathology. - 0275-6382 .- 1939-165X. ; 40, s. 52-59
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Tidskriftsartikel (refereegranskat)abstract
- Pyometra was associated with increased serum concentrations of the acute phase protein AGP. AGP concentrations were associated with severity of disease as measured by duration of hospitalization. As AGP binds basic drugs, further studies of its pharmacokinetic and pharmacodynamic propreties in cases of pyometra may be of clinical interest.
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| 2. |
- Hamlin, Helene, et al.
(författare)
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Detection of antinuclear antibodies by the Inno-Lia ANA update test in canine systemic rheumatic disease
- 2010
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Ingår i: Veterinary clinical pathology. - 0275-6382 .- 1939-165X. ; 39:2, s. 215-220
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Tidskriftsartikel (refereegranskat)abstract
- Background: Certain systemic autoimmune diseases in dogs are characterized by high titers of circulating antinuclear antibodies (ANA), which can be demonstrated by indirect immunofluorescence (IIF). In an earlier study of IIF-ANA-positive dogs, the Ouchterlony double immunodiffusion (DID) test was used to identify specific autoantibodies. The DID test has largely been replaced with line blot tests in human diagnostic settings. Objective: The objective of this study was to investigate whether the line blot assay Inno-Lia ANA update test is a useful tool in demonstrating ANA specificities in canine patients with previously diagnosed IIF-ANA-positive rheumatic disorders. Methods: Serum samples from 3 clinically healthy control dogs and 20 canine patients with clinical signs of systemic rheumatic disease and documented positive results for IIF-ANA and DID tests were included in the study. The Inno-Lia ANA update assay was performed with an anti-canine detection antibody. Results: Six serum samples that had DID positivity with anti-spliceosomal small nuclear ribonucleoproteins (snRNP) reactivity showed reactivity to multiple snRNP proteins in the Inno-Lia test. Samples from 2 dogs that had other types of DID positivity also had clear SmB reactivity and 1 had weak reactivity to RNP-70K. The other serum samples, including controls, were negative. Conclusions: Using the Inno-Lia ANA update test, multiple snRNP specificities were demonstrated in some canine patients with autoimmune rheumatic disorders. Other canine autoantibodies may exist that are not detected by this test. Further studies are necessary to characterize the target antigen(s) of these remaining autoantibodies in canine sera.
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| 3. |
- Hillström, Anna, et al.
(författare)
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Evaluation of cytologic findings in feline conjunctivitis
- 2012
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Ingår i: Veterinary Clinical Pathology. - 0275-6382 .- 1939-165X. ; 41, s. 283-290
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Tidskriftsartikel (refereegranskat)abstract
- Background Cytologic examination of smears prepared from ocular swabs of conjunctiva from cats with conjunctivitis permits identification of the type of inflammation and possibly specific microorganisms. Results of studies of the diagnostic utility of cytology for detection of infectious causes of feline conjunctivitis have been inconsistent. Objectives The objectives of this study were to describe cytologic findings in cats with conjunctivitis and to compare those findings with results of PCR analysis for feline herpesvirus (FHV-1), Chlamydophila felis (C felis), and Mycoplasma felis (M felis). Methods Conjunctival smears from 88 cats with conjunctivitis and 10 healthy control cats were stained with a Romanowsky stain and evaluated for the type of inflammation and evidence of an infectious agent. PCR analysis for FHV-1, C felis, and M felis was performed. Results Infectious agents identified by PCR analysis were FHV-1 in 9 cats (10%), C felis in 8 cats (9%), and M felis in 6 cats (7%). Inclusions interpreted as chlamydial inclusions were found in all cytologic smears from cats positive for C felis by PCR analysis and in 3 PCR-negative cats. Inclusions interpreted as Mycoplasma organisms were found in 3 of 6 cats that were PCR-positive for M felis and in 1 PCR-negative cat. FHV-1 inclusion bodies were not detected on cytologic examination. Conclusions Cytologic examination can be diagnostic for C felis infection when many typical inclusions are present. Cytologic examination was unreliable in diagnosing M felis infection, and viral inclusions of FHV-1 were not found in specimens stained with Romanowsky stains.
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| 6. |
- Lilliehöök, Inger, et al.
(författare)
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Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. I. Erythrocytes, platelets, and total leukocyte counts
- 2009
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Ingår i: Veterinary Clinical Pathology. - 0275-6382 .- 1939-165X. ; 38, s. 163-174
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Tidskriftsartikel (refereegranskat)abstract
- The Sysmex XT-2000iV performed as well as the CELL-DYN on blood samples from dogs, cats, and horses with a variety of hematologic abnormalities. In addition, the Sysmex detected large platelets and provided accurate reticulocyte counts.
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| 7. |
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| 8. |
- Strage, Emma, et al.
(författare)
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Validation of an enzyme-linked immunosorbent assay for measurement of feline serum insulin
- 2012
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Ingår i: Veterinary Clinical Pathology. - : Wiley. - 0275-6382 .- 1939-165X. ; 41, s. 518-528
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Tidskriftsartikel (refereegranskat)abstract
- Background Feline insulin has been measured previously using assays developed for measuring human insulin. As feline insulin differs from human insulin, it is important to validate the assay before use. Objectives The aims of this study were to validate an ELISA, the Mercodia Feline Insulin ELISA, intended for measuring feline insulin and to determine the stability of feline insulin in serum. Methods Validation of the ELISA, which uses monoclonal antibodies that recognize both human and feline insulin, included evaluation of coefficients of variation (CVs), patterns of variation, and consistency after dilution and spiking with feline insulin. Stability was evaluated by measuring insulin in feline serum samples stored at 20 degrees C, 28 degrees C, and -80 degrees C. Results The intra-assay CV in 1420 adjacent replicates (excluding position effects) was 2.04.2% and the inter-assay CV was 7.614%. The systematic and random position effect yielded a CV of 6.210%. When 3 feline serum samples were set at fixed positions and analyzed on 8 plates, microplate effects and interaction were significant for all 3 samples. Recovery upon dilution and spiking was 78105% and 86126%, respectively. Feline serum insulin concentration was stable for 24 hours at 20 degrees C, for 4 days at 28 degrees C, and for 15 months at -80 degrees C. Conclusions The Mercodia Feline Insulin ELISA can be used for measuring serum feline insulin. Recovery after spiking and dilution was acceptable. As in many ELISAs, intra-assay CV for adjacent replicates was low, whereas the position and between-assay CVs were considerably higher.
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