1. |
- Enejder, Annika, 1969, et al.
(författare)
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Dual-CARS microscopy
- 2007
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Ingår i: PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE). - 0277-786X. - 9780819465559 ; 6442:15
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Konferensbidrag (refereegranskat)abstract
- We present a new Coherent Anti-Stokes Raman Scattering (CARS) microscopy technique for label-free imaging of bio-molecules in living cells; dual-CARS microscopy. The use of three synchronized laser pulses in a dual-pump/dual-detection configuration enables imaging of two species with different molecular vibrations simultaneously, as well as acquisition of images free of non-resonant background. We show the power of the method by imaging deuterated nonadecane slowly diffusing into a suspension of living yeast cells in medium, clearly distinguishing the medium and the lipid droplets in the cells by probing the CH2 vibration from the D-nonadecane by probing the CD vibration. In addition, images of lipid stores in living C. elegans nematodes free of non-resonant background are shown. This results in a significant enhancement of the image contrast, allowing the visualization of emerging, low-density lipid stores in a dauer larva, difficult to distinguish in conventional CARS microscopy. The separation of the non-resonant background is shown to be beneficial also when monitoring molecules with weak vibrational modes. The improved sensitivity obtained is illustrated by probing the C=C vibration in polyunsaturated lipids extracted from fish. This enables the monitoring of the degree of unsaturation of lipids, a high value of which is reported in foods known to have positive effects on human health.
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2. |
- Abrahamsson, Christoffer, et al.
(författare)
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Scatter correction of transmission NIR spectra by photon migration data - Quantitative analysis of solids
- 2005
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 1996-756X .- 0277-786X. ; 6009, s. 60090-60090
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Konferensbidrag (refereegranskat)abstract
- The scope of this presentation is a new methodology to correct conventional NIR data for scattering effects. The technique aims at measuring the absorption coefficient of the samples rather than the total attenuation, measured by conventional NIR spectroscopy. The main advantage of this is that the absorption coefficient is independent of the path length of the light inside the sample, and therefore independent of the scattering effects. The measurements in this work were made using a novel system for time-resolved measurements, based on short light continuum pulses generated in an index-guided crystal fibre and a spectrometer-equipped streak camera. The system enables spectral recordings in the wavelength range 500 - 1200 nm with a spectral resolution of 5 nm and a temporal resolution of 30 ps. The evaluation scheme is based on modeling of light transport by diffusion theory, that provides an independent measure of the scattering properties of the samples, that later is used to correct conventional NIR data. This yields a clear advantage over other pre-processing techniques, where scattering effects are estimated and corrected for by using the shape of the measured spectrum only. PLS calibration models shows that, by using the proposed evaluation scheme, the predictive ability is improved by 50% as compared to models based on conventional NIR data. The method also makes it possible to predict the concentration of active substance in samples with physical properties different from those of the samples included in the calibration model.
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3. |
- Achilles, Silvio, et al.
(författare)
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GPU-Accelerated Coupled Ptychographic Tomography
- 2022
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Ingår i: Developments in X-Ray Tomography XIV. - : SPIE. - 1996-756X .- 0277-786X. - 9781510654686 ; 12242
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Konferensbidrag (refereegranskat)abstract
- Scanning coherent X-ray microscopy (ptychography) has gained considerable interest during the last decade since the performance of this indirect imaging technique does not necessarily rely on the quality of the X-ray optics and, in principle, can achieve highest spatial resolution in X-ray imaging. The method can be easily extended to 3D by adding standard tomographic reconstruction schemes. However, the tomographic reconstruction is often applied in a subsequent step using a sequence of aligned ptychographic 2D projections. In this contribution, we outline current developments of a GPU-accelerated framework for direct 3D ptychography, coupling 2D ptychography and tomography. The program utilizes a custom GPU-accelerated framework for ptychography that offers three distinct ptychographic reconstruction algorithms. The tomographic reconstruction runs simultaneously and uses numerical routines of the ASTRA Toolbox. This parallel-computing approach results in a high performance increase considerably reducing the reconstruction time of 3D ptychographic datasets.
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4. |
- Adler, Alexandre, et al.
(författare)
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Modelling ground pickup for microwave telescopes
- 2022
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Ingår i: Proceedings of SPIE, the International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. ; 12190
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Tidskriftsartikel (refereegranskat)abstract
- Microwave telescopes require an ever-increasing control of experimental systematics in their quest to measure the Cosmic Microwave Background (CMB) to exquisite levels of precision. One important systematic for ground and balloon-borne experiments is ground pickup, where beam sidelobes detect the thermal emission of the much warmer ground while the main beam is scanning the sky. This generates scan-synchronous noise in experiment timestreams, which is difficult to filter out without also deleting some of the signal from the sky. Therefore, efficient modelling of pickup can help guide the design of experiments and of analysis pipelines. In this work, we present an extension to the BEAMCONV algorithm that enables us to generate time-ordered data (TOD) from beam-convolved sky and ground maps simultaneously. We simulate ground pickup for both a ground-based experiment and a telescope attached to a stratospheric balloon. Ground templates for the balloon experiment are obtained by re-projecting satellite maps of the Earth's microwave emission.
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5. |
- Afanasiev, Sergey V., et al.
(författare)
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Experimental apparatus to study crystal channeling in an external SPS beamline
- 2007
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Ingår i: Proceedings of SPIE, the International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. ; 6634
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Tidskriftsartikel (refereegranskat)abstract
- For the new generation of high intensity hadronic machines as, for instance, LHC, halo collimation is a necessary issue for the accelerator to operate at the highest possible luminosity and to prevent the damage of superconductor magnets.1 We propose an experiment aimed to systematic study of the channeling phenomenology and of the newly observed "volume reflection" effect. This experiment will be performed for an external SPS beamline and will make use of a primary proton beam with 400 GeV/c momentum and very small (∼ 3 μrad) divergence. The advantage of a proposed experiment is precise tracking of particles that interacted with a crystal, so that to determine the single-pass efficiency for all the processes involved. For this purpose, a telescope equipped with high-resolution silicon microstrip detectors will be used. New generation silicon crystals and an extra-precise goniometer are mandatory issues. Main goal of the experiment is to get the precise information on channeling of relativistic particles and, ultimately, on the feasibility of such technique for halo collimation at LHC. In this contribution we review the status of the setting-up of experimental apparatus and its future development in sight of the planned run in September 2006.
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6. |
- Alexander, Naomi E., et al.
(författare)
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IMAGINE project : A low cost, high performance, monolithic passive mm-wave imager front-end
- 2012
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. - 9780819492852
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Konferensbidrag (refereegranskat)abstract
- The FP7 Research for SME project IMAGINE - a low cost, high performance monolithic passive mm-wave imager front-end is described in this paper. The main innovation areas for the project are: i) the development of a 94 GHz radiometer chipset and matching circuits suitable for monolithic integration. The chipset consists of a W-band low noise amplifier, fabricated using the commercially available OMMIC D007IH GaAs mHEMT process, and a zero bias resonant interband tunneling diode, fabricated using a patented epi-layer structure that is lattice matched to the same D007IH process; ii) the development of a 94 GHz antenna adapted for low cost manufacturing methods with performance suitable for real-time imaging; iii) the development of a low cost liquid crystal polymer PCB build-up technology with performance suitable for the integration and assembly of a 94 GHz radiometer module; iv) the assembly of technology demonstrator modules. The results achieved in these areas are presented.
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7. |
- Alfredson, J.
(författare)
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Individual differences in visual behaviour in simulated flight
- 2002
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Ingår i: Proceedings of SPIE, the International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. ; 4662, s. 494-502
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- Flying an aircraft is highly visually demanding. It is very important to map pilot visual behaviour, both for the purpose of evaluating the cockpit interface and to effectively integrate it with future adaptive interfaces and decision support systems. Pilots' visual behaviour was studied in two experiments. In the first experiment commercial aviation pilots were flying a commercial aviation scenario and eye point of gaze, and eye blinks were collected. In the second experiment military pilots were flying an air-to-air combat scenario and the visual behaviour was video recorded. In both of the experiments the results show individual differences in the pilots' visual behaviour. In the second experiment two different categories of eye blinks were found that might help explain the individual differences in visual behaviour. One category can be related to the systematic eye blinks found to occur when the eye point of gaze was changed between head-up/head-down and head-down/head-up. The other category could be related to other reasons, such as, mental workload or visual demands.
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8. |
- Anders, A., et al.
(författare)
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High speed and high dynamic range difference imaging based on the near sensor image processing concept
- 2009
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Ingår i: Proceedings of SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X. ; 7249, s. 72490G-
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Tidskriftsartikel (refereegranskat)abstract
- The paper describes the Near Sensor Image Processing (NSIP) paradigm developed in the early 1990s and shows that it was a precursor to recent architectures proposed for direct (in the sensor) image processing and high dynamic range (HDR) image sensing. Both of these architectures are based on the specific properties of CMOS light sensors, in particular the ability to continuously monitor the accumulation of photon-induced charge as a function of time. We further propose an extension of the original NSIP pixel to include a circuit that facilitates temporal and spatio-temporal processing.
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9. |
- Andersen, Torben, et al.
(författare)
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A new concept for large deformable mirrors for extremely large telescopes
- 2006
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Ingår i: Proceedings of the SPIE - The International Society for Optical Engineering. - : SPIE. - 1996-756X .- 0277-786X. ; 6272:1, s. 324-331
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Konferensbidrag (refereegranskat)abstract
- For extremely large telescopes, there is strong need for thin deformable mirrors in the 3-4 m class. So far, feasibility of such mirrors has not been demonstrated. Extrapolation from existing techniques suggests that the mirrors could be highly expensive. We give a progress report on a study of an approach for construction of large deformable mirrors with a moderate cost. We have developed low-cost actuators and deflection sensors that can absorb mounting tolerances in the millimeter range, and we have tested prototypes in the laboratory. Studies of control laws for mirrors with thousands of sensors and actuators are in good progress and simulations have been carried out. Manufacturing of thin, glass mirror blanks is being studied and first prototypes have been produced by a slumping technique. Development of polishing procedures for thin mirrors is in progress
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10. |
- Andersen, Torben, et al.
(författare)
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A parallel integrated model of the Euro50
- 2004
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Ingår i: Proceedings of the SPIE - The International Society for Optical Engineering. - : SPIE. - 0277-786X .- 1996-756X. ; 5497:1, s. 251-265
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Konferensbidrag (refereegranskat)abstract
- The Euro50 is an astronomical extremely large telescope for optical and infrared wavelength with a 50 m primary mirror. The telescope will have an elaborate control system ("live optics") to correct for atmospheric and telescope aberrations. To study and predict performance of the complete telescope system, an integrated model combining the structural model of the telescope, optics models, the control systems, and the adaptive optics has been established. Wind is taken into account on the basis of wind tunnel measurements and computer fluid dynamics calculations. Atmospheric aberrations are included using a seven-layer atmosphere model. The integrated model is written in Matlab and is run on a cluster computer to achieve acceptable execution times. Dedicated ordinary differential equation solvers have been written and a special toolkit for communication between Matlab processes on different nodes of the cluster computer has been set up. Preliminary results from the complete integrated model, including adaptive optics, are shown
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