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- Aili, Daniel, et al.
(författare)
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Synthetic de novo designed polypeptides for control of nanoparticle assembly and biosensing
- 2007
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Ingår i: Biochemical Society Transactions. - 0300-5127 .- 1470-8752. ; 35:3, s. 532-534
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Tidskriftsartikel (refereegranskat)abstract
- This contribution describes how de novo designed synthetic helix–loop–helix polypeptides are utilized tocontrol the assembly of gold nanoparticles and as scaffolds for biosensing. The synthetic polypeptides aredesigned to fold into a four-helix bundle upon dimerization. When immobilized on gold nanoparticles,dimerization and folding occur between peptides on neighbouring particles as an effect of particleaggregation and the folded polypeptides are rigid enough to keep the particles separated at a distancecorresponding to the size of the four-helix bundle. Moreover, peptide dimerization offers a convenientroute to assemble nanoparticles into hybrid multilayers on planar substrates. The drastic change in theresonance conditions of the localized nanoparticle surface plasmon upon particle aggregation is shown tobe useful for optical detection of biomolecular interactions.
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- Barg, Sebastian, 1969-, et al.
(författare)
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Granule docking and cargo release in pancreatic β-cells
- 2008
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Ingår i: Biochemical Society Transactions. - 0300-5127 .- 1470-8752. ; 36:Pt 3, s. 294-299
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Tidskriftsartikel (refereegranskat)abstract
- Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore.
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- Daniel, Chammiran, et al.
(författare)
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RNA editing and its impact on GABAa receptor function
- 2009
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Ingår i: Biochemical Society Transactions. - : Biochemical Society. - 0300-5127 .- 1470-8752. ; 37, s. 1399-1403
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Tidskriftsartikel (refereegranskat)abstract
- A-to-I (adenosine-to-inosine) RNA editing catalysed by the ADARs (adenosine deaminases that act on RNA) is a post-transcriptional event that contributes to protein diversity in metazoans. In mammalian neuronal ion channels, editing alters functionally important amino acids and creates receptor subtypes important for the development of the nervous system. The excitatory AMPA (α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid) and kainate glutamate receptors, as well as the inhibitory GABAA [GABA (γ-aminobutyric acid) type A] receptor, are subject to A-to-I RNA editing. Editing affects several features of the receptors, including kinetics, subunit assembly and cell-surface expression. Here, we discuss the regulation of editing during brain maturation and the impact of RNA editing on the expression of different receptor subtypes.
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