| 1. |
- Abrahamsson, P. ‐A, et al.
(författare)
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Three predominant prostatic proteins : Drei vorherrschende Prostataproteine
- 1990
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Ingår i: Andrologia. - : Hindawi Limited. - 0303-4569 .- 1439-0272. ; 22:1 S, s. 122-131
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Tidskriftsartikel (refereegranskat)abstract
- Summary Prostatic acid phosphatase (PAP), prostate‐specific antigen (PSA; or γ‐seminoprotein), and β‐microseminoprotein (β‐MSP; PSP94 or β‐inhibin) are the three predominant proteins secreted by the normal human prostate gland. In the epithelium of normal and hyperplastic prostatic acini and ducts PAP, PSA and β‐MSP have an identical immunohistochemical localization. Highly differentiated (grade I) carcinomas contain an almost equal number of PAP‐, PSA‐ and β‐MSP‐immunoreactive cells; the incidence of these cells is lower and they display a greater staining variability in the moderately and poorly (grade II‐III) differentiated tumours. Especially in poorly differentiated tumours PSA seems to be a more sensitive immunohistochemical marker than PAP or prostatic carcinomas. Moreover, the use of PAP as a marker for prostatic carcinomas is complicated by the reported structural similarities between the prostatic secreted acid phosphatase and lysosomal acid phosphatase occurring in all tissues. The use of β‐MSP as a marker for prostatic carcinomas may be limited by indications of non‐prostatic production of this protein.
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| 2. |
- Elzanaty, Saad, et al.
(författare)
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Seminal plasma albumin: origin and relation to the male reproductive parameters.
- 2007
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Ingår i: Andrologia. - : Hindawi Limited. - 0303-4569 .- 1439-0272. ; 39:2, s. 60-65
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Tidskriftsartikel (refereegranskat)abstract
- We wanted to investigate the origin of seminal plasma albumin and its relation to the male reproductive parameters. Semen samples from 916 men, under infertility assessment, were analysed according to guidelines of the World Health Organization. Seminal plasma constituents, i.e. albumin, markers of the epididymal (neutral alpha-glucosidase, NAG), prostatic (prostate-specific antigen, PSA, and zinc) and seminal vesicle function (fructose), as well as levels of reproductive hormones in plasma were measured. The sperm chromatin structure assay (SCSA) was applied on 267 of the 916 samples. A negative correlation was seen for seminal albumin and plasma follicle-stimulating hormone (r = -0.1, P = 0.02) and a positive correlation for seminal albumin and serum inhibin B (r = 0.2, P = 0.004). Albumin exhibited positive correlations with the epididymal marker, NAG (r = 0.5, P < 0.001) and with the prostatic markers, PSA and zinc (r = 0.1, P = 0.001; r = 0.2, P < 0.001 respectively) as well as with age (r = 0.2, P < 0.001). A negative significant association was seen for seminal albumin and semen volume (beta = -0.60; 95% CI -0.80 to -0.30). The opposite trend was found regarding sperm concentration (beta = 0.34; 95% CI 0.30-0.40), total sperm count (beta = 0.30; 95% CI 0.20-0.40), and percentage morphologically normal spermatozoa (beta = 0.70; 95% CI 0.10-1.0). No association was found between albumin and sperm motility, SCSA parameters, or fructose, the marker of seminal vesicles. Our results suggest testicular, epididymal and prostatic origin of seminal plasma albumin, in addition to the contribution from blood. This is the first study to demonstrate an association between seminal plasma albumin and sperm morphology. Further studies are needed to elucidate the role of seminal albumin in sperm morphology.
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| 3. |
- Giwercman, Aleksander, et al.
(författare)
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Testicular cancer and molecular genetics.
- 2005
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Ingår i: Andrologia. - : Hindawi Limited. - 0303-4569 .- 1439-0272. ; 37:6, s. 224-225
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Tidskriftsartikel (refereegranskat)
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| 4. |
- Lilja, H.
(författare)
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Cell biology of semenogelin
- 1990
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Ingår i: Andrologia. - : Hindawi Limited. - 0303-4569 .- 1439-0272. ; 22:1 S, s. 132-141
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Tidskriftsartikel (refereegranskat)abstract
- Summary Semenogelin is the predominant protein in human semen. It is synthetised by the secretory epithelium of the seminal vesicles as a 461 amino acid precursor protein. Following cleavage of a predominantly hydrophobic signal peptide, the secreted protein contains 439 amino acid residues. The amino acid sequence of semenogelin has a repetitive structure, but it displays no significant homology to any other characterized translation product. In the seminal vesicle secretion, semenogelin occurs in disulphide‐linked high molecular mass complexes together with two less abundant 71 and 76 kDa proteins. These proteins have a very close antigenic and structural relationship to semenogelin, but contrary to semenogelin one or both of these semenogelin‐related proteins are also expressed by the secretory epithelium of the epididymis. Semenogelin and the semenogelin‐related proteins are the major proteins involved in the gelatinous entrapment of ejaculated spermatozoa. Antigenic epitopes common to these proteins are localised to the locomotive parts of the spermatozoa. The spermatozoa become progressively motile when gel‐forming proteins are fragmented by the kallikrein‐like protease, prostate‐specific antigen, and the gel dissolves.
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| 5. |
- Inayat, S, et al.
(författare)
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High levels of cathepsins B, L and S in human seminal plasma and their association with prostasomes
- 2012
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Ingår i: Andrologia. - : Hindawi Limited. - 0303-4569 .- 1439-0272. ; 44:6, s. 423-427
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Tidskriftsartikel (refereegranskat)abstract
- Semen is a heterogenous and complex fluid with different functions, some of them well known, others still obscure. The aim of this study was to investigate the presence of cathepsins B, S and L in human seminal plasma and their possible associations with other semen variables. Cathepsin B, L and S concentrations were measured in seminal plasma from 99 men utilising commercial ELISA kits. Seminal plasma cathepsin B was approximately 70 times higher, while the cathepsin L values were approximately 500 times higher and the cathepsin S values approximately 40 times higher in seminal plasma than in a group of serum samples. The study shows that seminal plasma contains high levels of cathepsins B, L and S. All three cathepsins were also bound to the surface of prostasomes.
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| 6. |
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| 8. |
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| 9. |
- Spjuth, L., et al.
(författare)
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Effects of exposure of pre-pubertal boars to di(2-ethylhexyl) phthalate on their frozen-thawed sperm viability post-puberty
- 2006
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Ingår i: Andrologia. - : Wiley-Blackwell. - 0303-4569 .- 1439-0272. ; 38:5, s. 186-194
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Tidskriftsartikel (refereegranskat)abstract
- Late effects of pre-pubertal oral exposure to di(2-ethylhexyl) phthalate (DEHP), a plastic softener used in, for example, polyvinyl chloride-products, on semen quality in young boars have not been clear-cut. The aim of this study was to determine whether stress imposed on spermatozoa would reveal such effects. Semen was collected from post-pubertal boars (8-9 months of age), which had been exposed to 300 mg kg(-1) body weight of DEHP per os three times a week from 3 to 7 weeks of age and from control siblings given placebo (water). The semen was cryopreserved and examined for plasma membrane integrity post-thaw using the short hypo-osmotic swelling test and flow cytometry (propidium iodide /SYBR-14). Sperm motility was assessed by computer-assisted sperm analysis. No significant difference in plasma membrane integrity could be found between the groups. The DEHP-exposed group had a significantly lower percentage of linearly motile spermatozoa at 30 min (P less than 0.05) and 120 min (P less than 0.001) after thawing, and a larger amplitude of lateral displacement of the head 120 min after thawing (P less than 0.05), compared with controls. In summary, spermatozoa from boars pre-pubertally exposed to low doses of DEHP, showed kinematic deviations post-thaw that could be related to DEHP exposure.
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