| 1. |
- Alm, Gunnar V., et al.
(författare)
-
The in vitro maturation of the embryonic chicken thymus, 1: : Development of an Organ Culture System
- 1972
-
Ingår i: Acta Pathologica Microbiologica Scandinavica, Section A Pathology. - : Wiley. - 0365-4184. ; A 80:6, s. 778-784
-
Tidskriftsartikel (refereegranskat)abstract
- An organ culture technique is described which permits the lymphoid development of the thymic anlagen of 10-day-old chick embryos for at least 10 days in vitro. Grid organ cultures of a modified Trowell type were employed. Among several tissue culture media tested, Waymouth's MB 752/1 medium gave superior results. In initial experiments this medium was supplemented with heat-inactivated horse serum and embryo-extract. Subsequently it was found that this supplement could be substituted for by chicken serum alone. The morphological development of the embryonic thymic anlagen in organ culture has been characterized. Typical lymphoid cells developed from the initial lymphoid precursor cells. Late in the culture period, large numbers of small lymphocytes appeared. Extensive cell proliferation was demonstrated during the first 6 days of culture by means of autoradiography. This organ culture technique should facilitate the further analysis of factors influencing thymic lymphocytopoiesis as well as of the role of the thymus in the development of lymphocyte functions.
|
|
| 2. |
- Cerecetto, Hugo, et al.
(författare)
-
1, 2, 4-Triazine N-oxide derivatives : studies as potential hypoxic cytotoxins. Part III.
- 2004
-
Ingår i: Archiv der Pharmazie. - : Wiley. - 0365-6233 .- 1521-4184. ; 337:5, s. 271-80
-
Tidskriftsartikel (refereegranskat)abstract
- New 5-(2-arylethenyl)-1, 2, 4-triazine N-oxide and N, N'-dioxide derivatives were synthesized in order to obtain compounds as selective hypoxic cell cytotoxins. The desired products were obtained when the 5-methyl heterocycle reacted with the corresponding iminium electrophiles. The new compounds were tested for their cytotoxicity in oxia and hypoxia. Some of them proved to be less active in hypoxic conditions than Tirapazamine, 3-aminobenzo[1, 2-e]1, 2, 4-triazine N(1), N(4)-dioxide. Derivative 11, 6-methyl-5-[2-(5-nitrofuryl)ethenyl)-1, 2, 4-triazine N(4)-oxide, was the most cytotoxic compound, but it was non-selective. Some derivatives were studied as DNA-binding agents in oxic conditions showing poor affinity for this biomolecule. This result showed that the cytotoxic activity in oxia is DNA damage not dependent. Electrochemical and ESR spectroscopy studies were performed in order to determine the ability of compounds to produce radicals and the relation of these in the mechanism of cytotoxicity.
|
|
| 3. |
- Cerecetto, Hugo, et al.
(författare)
-
1, 2, 4-Triazine N-oxide derivatives : studies as potential hypoxic cytotoxins. Part II.
- 2004
-
Ingår i: Archiv der Pharmazie. - : Weinheim Verlag. - 0365-6233 .- 1521-4184. ; 337:5, s. 247-258
-
Tidskriftsartikel (refereegranskat)abstract
- New 1, 2, 4-Triazine N-oxide and N, N'-dioxide derivatives were synthesized in order to obtain compounds as selective hypoxic cell cytotoxins. The starting heterocycles have been prepared using a standard microwave oven in a clean and good-yielded process. The reactivity of methyl-1, 2, 4-triazine N(4)-oxide and N(1), N(4)-dioxide with different electrophilic agents has been studied. The desired products were obtained only when iminium electrophiles were employed. The regioselectivity of this process has been studied by means of experimental and theoretical (at ab initio level) procedures. Theoretically was expected that the most stable intermediates where the benzylic-like anion from position 5. A fact which agreed with the experimental observed regioselectivity. The new compounds were tested for their cytotoxicity in oxia and hypoxia. Some of them proved to be less active in hypoxic conditions than tirapazamine, 3-amino-benzo[1, 2-e]1, 2, 4-triazine N(1), N(4)-dioxide. Derivative 19, 6-methyl-5-[2-(5-nitrothienyl)ethenyl)-1, 2, 4-triazine N(4)-oxide, was the most cytotoxic compound, but it was non-selective.
|
|
| 4. |
- Proszenyák, Agnes, et al.
(författare)
-
Synthesis, antimicrobial and antineoplastic activities for agelasine and agelasimine analogs with a beta-cyclocitral derived substituent.
- 2007
-
Ingår i: Archiv der Pharmazie. - : Wiley. - 0365-6233 .- 1521-4184. ; 340:12, s. 625-634
-
Tidskriftsartikel (refereegranskat)abstract
- Agelasines and agelasimines are antimicrobial and cytotoxic purine derivatives isolated from marine sponges (Agelas sp.). We have synthesized structurally simplified analogs of these natural products starting from beta-cyclocitral. The novel compounds were found to be strong inhibitors of a wide variety of pathogenic microorganisms (incl. Mycobacterium tuberculosis) as well as cancer cell lines. The biological activities were generally in the same range as those previously found for the structurally more complex agelasines and agelasimines isolated in small amounts from natural sources. We also report for the first time that agelasine and agelasimine analogs inhibit growth of protozoa (Acanthamoeba castellanii and Acanthamoeba polyphaga). Acanthamoeba keratitis is an increasingly common and severe corneal infection, closely associated with contact lens wear.
|
|
| 5. |
- Roggen, Heidi, et al.
(författare)
-
Antimicrobial and antineoplastic activities of Agelasine analogs modified in the purine 2-position
- 2011
-
Ingår i: Archiv der Pharmazie. - : Wiley. - 0365-6233 .- 1521-4184. ; 344:1, s. 50-55
-
Tidskriftsartikel (refereegranskat)abstract
- Agelasines are 7,9-dialkylpurinium salts found in marine sponges (Agelas sp.), which display a variety of antimicrobial and cytotoxic effects. We have synthesized simplified agelasine analogs modified in the purine 2-position and examined their antimicrobial and anticancer activities. The compounds were screened against Staphylococcus aureus, Escherichia coli, Mycobacterium tuberculosis, Candida krusei, and Candida albicans, protozoa causing tropical diseases (Plasmodium falciparum, Leishmania infantum, Trypanosoma cruzi, and Trypanosoma brucei), a panel of human cancer cell lines (U-937 GTB, RPMI 8226/s, CEM/s, and ACHN) as well as VERO and/or MRC-5 cells. The results indicate that the introduction of a methyl group in the purine 2-position is beneficial for antimycobacterial and antiprotozoal activity, and that amino groups may enhance activity against several cancer cell lines.
|
|
| 6. |
- Westermark, Per
(författare)
-
Occurrence of amyloid deposits in the skin in secondary systemic amyloidosis
- 1972
-
Ingår i: Acta Pathologica Microbiologica Scandinavica, Section A Pathology. - : Wiley. - 0365-4184. ; A 80:6, s. 718-720
-
Tidskriftsartikel (refereegranskat)abstract
- Among 9 patients with secondary systemic amyloidosis, 8 had amyloid deposits in the skin. Amyloid was found in the deepest part of the dermis around the adnexa and in the subcutaneous tissue in the form of rings around fat cells. The amount of amyloid varied somewhat between different skin areas and, among the parts of the body examined, the scalp and the abdominal skin showed the heaviest infiltration. It is obvious that secondary amyloidosis can be diagnosed by means of skin biopsy in many cases.
|
|
