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- Bejai, Sarosh
(författare)
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Isolation, Cloning and Characterization of Resistance Gene Analogues in Pearl Millet Based on Conserved Nucleotide-binding Sites
- 2011
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Ingår i: Journal of Phytopathology. - : Wiley. - 0931-1785 .- 1439-0434. ; 159, s. 382-389
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Tidskriftsartikel (refereegranskat)abstract
- Sequence analysis of plant disease resistance genes shows similarity among themselves, with the presence of conserved motifs common to the nucleotide-binding site (NBS). Oligonucleotide degenerate primers designed from the conserved NBS motifs encoded by several plant disease resistance genes were used to amplify resistance gene analogues (RGAs) corresponding to the NBS sequences from the genomic DNA of various plant species. Using specific primers designed from the conserved NBS regions, 22 RGAs were cloned and sequenced from pearl millet (Pennisetum glaucum L. Br.). Phylogenetic analysis of the predicted amino acid sequences grouped the RGAs into nine distinct classes. GenBank database searches with the consensus protein sequences of each of the nine classes revealed their conserved NBS domains and similarity to other known R genes of various crop species. One RGA 213 was mapped onto LG1 and LG7 in the pearl millet linkage map. This is the first report of the isolation and characterization of RGAs from pearl millet, which will facilitate the improvement of marker-assisted breeding strategies.
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| 2. |
- Hu, Yang, et al.
(författare)
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Characterization of a Heterobasidion irregulare endo-rhamnogalacturonase that mediate growth on pectin
- 2018
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Ingår i: Journal of Phytopathology. - : Wiley. - 0931-1785 .- 1439-0434. ; 166, s. 34-43
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Tidskriftsartikel (refereegranskat)abstract
- Heterobasidion irregulare is one of five Heterobasidion annosum sensu lato (s.l.) species, which are destructive pathogens in boreal and temperate forests of the northern hemisphere that causes root and butt rot in conifer. A gene encoding endo-rhamnogalacturonase (HIRHG), which belongs to the glycoside hydrolase family 28 (GH28), was found in a quantitative trait loci (QTL) region for virulence in Heterobasidion. In this study, we showed that HIRHG is highly upregulated during necrotrophic infection of Norway spruce compared with growth in liquid culture and that the HIRHG encoded protein is produced during fungal growth on complex carbon sources. Phylogenetic analysis of endo-rhamnogalacturonases revealed that rhamnogalacturonase genes have been lost in most of the biotrophic and hemibiotrophic plant pathogens investigated but were common in necrotrophic pathogens and saprophytic fungi. Heterologous expression of the HIRHG gene in the hemibiotrophic fungus Magnaporthe oryzae increased its capacity to grow on pectin; however, the transformed M.oryzae isolates showed significant less infection of rice leaves compared to the wild type.
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