| 1. |
- Baltzer, Lars, et al.
(author)
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Emerging principles of de novo catalyst design
- 2001
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In: Current Opinion in Biotechnology. - 0958-1669 .- 1879-0429. ; 12:4, s. 355-360
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Research review (peer-reviewed)abstract
- Considerable progress has been made in the understanding of how to exploit hydrophobic and charge - charge interactions in forming binding sites for peptides and small molecules in folded polypeptide catalysts. This knowledge has enabled the introduction of feedback and control functions into catalytic cycles and the construction of folded polypeptide catalysts that follow saturation kinetics. Major advances have also been made in the design of metalloproteins and metallopeptides, especially with regards to understanding redox potential control.
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| 2. |
- Brodelius, Peter
(author)
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Enzyme assays
- 1991
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In: Current Opinion in Biotechnology. - 0958-1669 .- 1879-0429. ; 2:1, s. 23-29
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Journal article (peer-reviewed)abstract
- The past year or so has seen the development of new enzyme assays, as well as the improvement of existing ones. Assays are becoming more rapid and sensitive as a result of modifications such as amplification of the enzyme product(s). Recombinant DNA technology is now being recognized as a particularly useful tool in the search for improved assay systems.
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| 3. |
- Hult, Karl, et al.
(author)
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Engineered enzymes for improved organic synthesis
- 2003
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In: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 14:4, s. 395-400
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Research review (peer-reviewed)abstract
- Recent developments to modify enzymes for use in organic synthesis have targeted several areas. These include altering the reaction mechanism of the enzyme to catalyse new reactions, switching substrate specificity, expanding substrate specificity, and improving substrate specificity, such as enantioselectivity in kinetic resolutions. Such modifications can be achieved either by rational redesign, which requires knowledge of the enzyme structure, or by random mutagenesis methods followed by screening. Both strategies of enzyme engineering can be successful and are very useful for improving the utility of enzymes for applied catalysis. Several examples illustrating these concepts in a variety of enzyme classes have appeared recently.
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| 4. |
- Andersson, Helene, et al.
(author)
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Microtechnologies and nanotechnologies for single-cell analysis
- 2004
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In: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 15:1, s. 44-49
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Research review (peer-reviewed)abstract
- Many efforts are currently underway to try and mimic the properties of single cells with the aim of designing chips that are as efficient as cells. However, cells are nature's nanotechnology engineering at the scale of atoms and molecules, and it might be better to envision a microchip that utilizes a single cell as an experimentation platform. A novel, so-called laboratory-in-a-cell concept has been described, where advantage is taken of micro-and nanotechnological tools to enable precise control of the biochemical cellular environment; these tools also offer the possibility to analyse the composition of single cells. Methods for single-cell handling and analysis are being developed and will be required for this concept to progress further.
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| 5. |
- Angermayr, S. Andreas, et al.
(author)
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Energy biotechnology with cyanobacteria
- 2009
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In: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 20:3, s. 257-263
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Research review (peer-reviewed)abstract
- The world's future energy demand calls for a sustainable alternative for the use of fossil fuels, to restrict further global warming. Harvesting solar energy via photosynthesis is one of Nature's remarkable achievements. Existing technologies exploit this process for energy 'production' via processing of, for example, part of plant biomass into ethanol, and of algal biomass into biodiesel. Fortifying photosynthetic organisms with the ability to produce biofuels directly would bypass the need to synthesize all the complex chemicals of 'biomass'. A promising way to achieve this is to redirect cyanobacterial intermediary metabolism by channeling (Calvin cycle) intermediates into fermentative metabolic pathways. This review describes this approach via the biosynthesis of fermentation end products, like alcohols and hydrogen, driven by solar energy, from water (and CO2).
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| 6. |
- Björk, Sara, et al.
(author)
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Microfluidics for cell factory and bioprocess development
- 2019
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In: Current Opinion in Biotechnology. - : Elsevier. - 0958-1669 .- 1879-0429. ; 55, s. 95-102
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Journal article (peer-reviewed)abstract
- Bioindustry is expanding to an increasing variety of food, chemical and pharmaceutical products, each requiring rapid development of a dedicated cell factory and bioprocess. Microfluidic tools are, together with tools from synthetic biology and metabolic modeling, being employed in cell factory and bioprocess development to speed up development and address new products. Recent examples of microfluidics for bioprocess development range from integrated devices for DNA assembly and transformation, to high throughput screening of cell factory libraries, and micron scale bioreactors for process optimization. These improvements act to improve the biotechnological engineering cycle with tools for building, testing and evaluating cell factories and bioprocesses by increasing throughput, parallelization and automation.
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| 7. |
- Blomberg, Anders, 1956
(author)
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Measuring growth rate in high-throughput growth phenotyping.
- 2010
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In: Current opinion in biotechnology. - : Elsevier BV. - 1879-0429 .- 0958-1669. ; 22:1, s. 94-102
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Research review (peer-reviewed)abstract
- Growth rate is an important variable and parameter in biology with a central role in evolutionary, functional genomics, and systems biology studies. In this review the pros and cons of the different technologies presently available for high-throughput measurements of growth rate are discussed. Growth rate can be measured in liquid microcultivation of individual strains, in competition between strains, as growing colonies on agar, as division of individual cells, and estimated from molecular reporters. Irrespective of methodology, statistical issues such as spatial biases and batch effects are crucial to investigate and correct for to ensure low false discovery rates. The rather low correlations between studies indicate that cross-laboratory comparison and standardization are pressing issue to assure high-quality and comparable growth-rate data.
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| 8. |
- Cao, Zhixing, et al.
(author)
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Multi-scale data-driven engineering for biosynthetic titer improvement
- 2020
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In: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 65, s. 205-212
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Research review (peer-reviewed)abstract
- Industrial biosynthesis is a very complex process which depends on a range of different factors, from intracellular genes and metabolites, to extracellular culturing conditions and bioreactor engineering. The identification of species that improve the titer of some reaction is akin to the task of finding a needle in a haystack. This review aims to summarize state-of-the-art biosynthesis titer improvement on different scales separately, particularly regarding the advancement of metabolic pathway rewiring and data-driven process optimization and control. By integrating multi-scale data and establishing a mathematical replica of a real biosynthesis, more refined quantitative insights can be gained for achieving a higher titer than ever.
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| 9. |
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| 10. |
- Chen, Yun, 1978, et al.
(author)
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Advances in metabolic pathway and strain engineering paving the way for sustainable production of chemical building blocks
- 2013
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In: Current Opinion in Biotechnology. - : Elsevier BV. - 0958-1669 .- 1879-0429. ; 24:6, s. 965-972
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Research review (peer-reviewed)abstract
- Bio-based production of chemical building blocks from renewable resources is an attractive alternative to petroleum-based platform chemicals. Metabolic pathway and strain engineering is the key element in constructing robust microbial chemical factories within the constraints of cost effective production. Here we discuss how the development of computational algorithms, novel modules and methods, omics-based techniques combined with modeling refinement are enabling reduction in development time and thus advance the field of industrial biotechnology. We further discuss how recent technological developments contribute to the development of novel cell factories for the production of the building block chemicals: adipic acid, succinic acid and 3-hydroxypropionic acid.
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