| 1. |
- Deswal, Renu, et al.
(författare)
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Plant proteomics in India and Nepal : Current status and challenges ahead
- 2013
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Ingår i: Physiology and Molecular Biology of Plants. - : Springer-Verlag New York. - 0971-5894 .- 0974-0430. ; 19:4, s. 461-477
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Forskningsöversikt (refereegranskat)abstract
- Plant proteomics has made tremendous contributions in understanding the complex processes of plant biology. Here, its current status in India and Nepal is discussed. Gel-based proteomics is predominantly utilized on crops and non-crops to analyze majorly abiotic (49 %) and biotic (18 %) stress, development (11 %) and post-translational modifications (7 %). Rice is the most explored system (36 %) with major focus on abiotic mainly dehydration (36 %) stress. In spite of expensive proteomics setup and scarcity of trained workforce, output in form of publications is encouraging. To boost plant proteomics in India and Nepal, researchers have discussed ground level issues among themselves and with the International Plant Proteomics Organization (INPPO) to act in priority on concerns like food security. Active collaboration may help in translating this knowledge to fruitful applications.
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| 2. |
- Gholami, Soheila, et al.
(författare)
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Exploring genetic variations in threatened medicinal orchids using start codon targeted (SCoT) polymorphism and marker-association with seed morphometric traits
- 2021
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Ingår i: Physiology and Molecular Biology of Plants. - : Springer Nature. - 0971-5894 .- 0974-0430. ; 27:4, s. 769-785
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Tidskriftsartikel (refereegranskat)abstract
- We aimed to study the genetic diversity and population structure of eight Iranian terrestrial orchid species, including Anacamptis coriophora (L.) R. M. Bateman, Pridgeon and M. W. Chase, Dactylorhiza umbrosa (Kar. & Kir.) Nevski, Himantoglossum affine (Boiss.) Schltr., Orchis collina Banks and Solander, Orchis mascula (L.) L., Orchis simia Lam., Ophrys schulzei Bornm. and Fleischm., and Ophrys straussii H. Fleischm. and Bornm. using start target codon markers (SCoT) and finding markers associated with seed morphometric traits. A total of 254 reproducible SCoT fragments were generated, of which 248 fragments were polymorphic (average polymorphism of 96.18%). The SCoT markers showed a narrow range of polymorphism information content (PIC) varied from 0.397 for S9 primer to 0.499 for S11 and S20 primers. Based on the population analysis results, the Orchis simia accessions collected from Paveh region (Os.P) represented the lowest observed number of alleles (Na) (1.13) and effective number of alleles (Ne) (1.09). At the same time, the highest Na (1.29) and Ne (1.18) values were obtained in O. schulzei collected from Javanrood (Oyst.JA). Shannon's information index (I) was ranged from 0.03 for D. umbrosa accessions collected from Marivan (Du.M population) to 0.263 for Ha.Ja population (H. affine accessions collected from Javanrood). The UPGMA dendrogram obtained with the Jaccard similarity coefficient (r = 0.97295) divided 97 studied terrestrial orchid accessions into eight groups mainly based on species type and geographical origin. Based on the Bayesian statistical index, the highest probability of the data was achieved when accessions were divided into eight groups (K = 8). Multiple association analysis (MRA) revealed significant associations between some of SCoT bands with seed morphometric traits. Our findings can be useful for germplasm characterization, conservation, and improvement of Iranian terrestrial orchid species.
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| 3. |
- Gholami, Soheila, et al.
(författare)
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Molecular characterization of endangered Iranian terrestrial orchids using ISSR markers and association with floral and tuber-related phenotypic traits
- 2021
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Ingår i: PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS. - : Springer. - 0971-5894 .- 0974-0430. ; 27:1, s. 53-68
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Tidskriftsartikel (refereegranskat)abstract
- We aimed to study the genetic diversity, population structure, and phylogeny of Iranian orchids using inter-simple sequence repeat (ISSR) markers to find markers associated with phenotypic traits. Based on the phenotypic analysis, the inflorescence length and the flower number of studied accessions ranged from 3.92 to 27.13 cm and 5 to 50, respectively. On the other hand, the tuber length ranged from 1.80 to 9.35 cm. A total of 310 reproducible ISSR fragments with a size range of 150 to 3000 bp were amplified. ISSR primers provided an average polymorphism information content of 0.391, varied from 0.488 for UBC-876 to 0.351 for UBC-842. Os.J population showed the lowest genetic diversity (H = 0.057 and I = 0.075), while Oyst.JA population showed the highest genetic diversity (H = 0.114 and I = 0.158). At species level, the average coefficient of genetic differentiation (G(ST)) ranged from 0.265 for Orchis simia to 0.587 for Himantoglossum affine. Gene flow (Nm) varied from 1.38 (O. simia) to 0.756 (Anacamptis collina). The UPGMA genetic similarity dendrogram using Jaccard coefficients (r = 0.973) revealed six main clusters. Based on the Bayesian clustering method, the highest probability of the data was achieved when accessions were divided into eight groups. Floral and tuber-related phenotypic traits represented high correlations together, and they were associated with some ISSR bands based on the multiple association analysis. Altogether, ISSR markers proved to be useful for discrimination and clarification of the relationships among species and populations collected from geographically different locations. Furthermore, it could identify the polymorphism among accessions within each population and species.
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| 4. |
- Srivastava, R., et al.
(författare)
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Analysis of glutamine synthetase (glnA) gene from prokaryotic and eukaryotic organisms
- 2004
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Ingår i: Physiology and Molecular Biology of Plants. - 0971-5894. ; 10:2, s. 197-202
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Tidskriftsartikel (refereegranskat)abstract
- Glutamine synthetase (GS) is a key enzyme involved in nitrogen metabolism that performs the essential biochemical function of ammonium assimilation and glutamine synthesis. The enzyme and its isoforms are present universally in all organisms and display diverse regulatory patterns. In this study, we have analyzed some sequences upstream and downstream the initiation codon of the structural gene of GS (glnA) to show that these sequences are involved in regulation and stable expression of the enzyme. GS is known to be regulated by adenylylation-deadenylylation cascade in some organisms. Analysis of the adenylylation site from several organisms revealed that the site could also be deciphered from those organisms where regulation of the enzyme is not known by adenylylation. The adenylylation site was mutated by the use of Swiss-PdbViewer and possible reasons were assigned to the functional and nonfunctional properties of this site in various organisms. This analysis has also helped in assigning functional relationships to some conserved sequences within and in the proximity of the glnA gene.
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