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- Cadirci, Kenan, et al.
(författare)
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In Vitro Cytotoxic, Genotoxic, Embryotoxic and Oxidative Damage Potentials by Empagliflozin
- 2024
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Ingår i: Biology Bulletin of the Russian Academy of Science. - : Pleiades Publishing Ltd. - 1062-3590 .- 1608-3059. ; 51:2, s. 243-250
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Tidskriftsartikel (refereegranskat)abstract
- Empagliflozin (EMPA) is a potent, competitive and selective sodium glucose cotransporter-2 (SGLT-2) inhibitor that ameliorates blood glucose with the insulin-independent manner. EMPA reduces weight and blood pressure of patients with type 2 diabetes mellitus (T2DM) without developing hypoglycemic risk. To the best of our knowledge, its safety profiling has not been evaluated on human blood cell cultures yet. Again, the embryotoxicity potential by EMPA is still unclear. Therefore, in this investigation we aimed to evaluate the in vitro cytotoxic, genotoxic and embryotoxic damage potential as well as antioxidative/oxidative effects by EMPA in cultured human blood and human pluripotent embryonal carcinoma NT2 cells for the first time. Cell cultures (n = 5) were exposed to different concentrations ranging from 3.25 to 100 mg/L of EMPA for 48 and 72 h. Cell viability was measured by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) release assays. The alterations in antioxidant/oxidant activity were monitored via measuring the total antioxidant capacity (TAC) and total oxidative stress (TOS) levels. For evaluating the genotoxicity of EMPA chromosomal aberration (CA) assay was performed. The present results revealed that EMPA did not induce cytotoxic or genotoxic damage on healthy human blood cells. Moreover, EMPA exerted non-embryotoxic property and supported antioxidative capacity and decreased the oxidative stress in cultured human blood cells. Our results supported the safe and advantageous use of EMPA for the treatment of T2DM.
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| 3. |
- Tchebakova, N. M., et al.
(författare)
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Energy and Mass Exchange and the Productivity of Main Siberian Ecosystems (from Eddy Covariance Measurements). 2. Carbon Exchange and Productivity
- 2015
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Ingår i: Biology Bulletin of the Russian Academy of Science. - 1062-3590. ; 42:6, s. 579-588
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Tidskriftsartikel (refereegranskat)abstract
- Direct measurements of CO2 fluxes by the eddy covariance method have demonstrated that the examined middle-taiga pine forest, raised bog, true steppe, and southern tundra along the Yenisei meridian (similar to 90 degrees E) are carbon sinks of different capacities according to annual output. The tundra acts as a carbon sink starting from June; forest and bog, from May; and steppe, from the end of April. In transitional seasons and winter, the ecosystems are a weak source of carbon; this commences from September in the tundra, from October in the forest and bog, and from November in the steppe. The photosynthetic productivity of forest and steppe ecosystems, amounting to 480-530 g C/(m(2) year), exceeds by 2-2.5 times that of bogs and tundras, 200-220 g C/(m(2) year). The relationships between the heat balance structure and CO2 exchange are shown. Possible feedback of carbon exchange between the ecosystems and atmosphere as a result of climate warming in the region are assessed.
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