| 1. |
- Edqvist, Per-Henrik, et al.
(författare)
-
Early identification of retinal subtypes in the developing, pre-laminated chick retina using the transcription factors Prox1, Lim1, Ap2α, Pax6, Isl1, Isl2, Lim3 and Chx10
- 2006
-
Ingår i: European journal of histochemistry. - 1121-760X .- 2038-8306. ; 50:2, s. 147-154
-
Tidskriftsartikel (refereegranskat)abstract
- In this study, antibodies toward the transcription factors Prox1, Lim1, Ap2a, Pax6, Isl1, Isl2, Lim3 and Chx10 were used to identify and distinguish between developing cell types in the pre-laminated chick retina. The spatio-temporal expression patterns were analysed from embryonic day 3 (E3) to E9, thus covering a time-span from the onset of retinal cell-fate determination to when retinal laminas can be distinguished. Most transcription factors were found at early stages of development, enabling us to trace various precursor cell populations throughout the lamination process. With time, each transcription factor expression became restricted to distinct laminas or sub-laminas of the maturing retina. These early emerging patterns were compared and found to be consistent with those of the hatched chick retina, where the outer nuclear layer label for Lim3, Isl1 and Isl2. In the inner nuclear layer, horizontal cells labeled for Prox1, Lim1, Isl1, Ap2a and Pax6, bipolar cell labeled for Lim3, Isl1 and Chx10 and amacrine cells labeled for Ap2a, Isl1 and Pax6. The ganglion cell layer labeled for Isl1, Pax6 and Isl2. The immunolabeling patterns of Lim3 and Isl2 have not previously been described in detail.
|
|
| 2. |
- Hansson, J, et al.
(författare)
-
Species diversity regarding the presence of proximal tubular progenitor cells of the kidney
- 2016
-
Ingår i: European Journal of Histochemistry. - : PAGEPress Publications. - 2038-8306 .- 1121-760X. ; 60:1, s. 2567-2567
-
Tidskriftsartikel (refereegranskat)abstract
- The cellular source for tubular regeneration following kidney injury is a matter of dispute, with reports suggesting a stem or progenitor cells as the regeneration source while linage tracing studies in mice seemingly favor the classical theory, where regeneration is performed by randomly surviving cells. We, and others have previously described a scattered cell population localized to the tubules of human kidney, which increases in number following injury. Here we have characterized the species distribution of these proximal tubular progenitor cells (PTPCs) in kidney tissue from chimpanzee, pig, rat and mouse using a set of human PTPC markers. We detected PTPCs in chimpanzee and pig kidneys, but not in mouse tissue. Also, subjecting mice to the unilateral urethral obstruction model, caused clear signs of tubular injury, but failed to induce the PTPC phenotype in renal tubules.
|
|
| 3. |
- Jennische, Eva, 1949, et al.
(författare)
-
Dark-field microscopy enhance visibility of CD31 endothelial staining
- 2020
-
Ingår i: European journal of histochemistry : EJH. - : PAGEPress Publications. - 2038-8306 .- 1121-760X. ; 64:3
-
Tidskriftsartikel (refereegranskat)abstract
- A simple dark field microscopy technique was used for visualization of blood vessels in normal human renal tissues and carcinoma. Phase contrast condenser ring apt for high power objectives was combined with a 10x objective in order to create a dark field illumination of the specimens examined. The endothelial lining of the vessels had been stained by using CD31 monoclonal antibodies combined with conventional peroxidase immunohistochemistry. The final DAB addition used for this technique induced an intense light scatter in the dark field microscope. This scattered light originating from the endothelial lining made the walls of the bright vessels easily detectable from the dark background.
|
|
| 4. |
- Larsen, AK, et al.
(författare)
-
Autofluorescence in freshly isolated adult human liver sinusoidal cells
- 2021
-
Ingår i: European journal of histochemistry : EJH. - : PAGEPress Publications. - 2038-8306 .- 1121-760X. ; 65:4
-
Tidskriftsartikel (refereegranskat)abstract
- Autofluorescent granules of various sizes were observed in primary human liver endothelial cells (LSECs) upon laser irradiation using a wide range of wavelengths. Autofluorescence was detected in LAMP-1 positive vesicles, suggesting lysosomal location. Confocal imaging of freshly prepared cultures and imaging flow cytometry of non-cultured cells revealed fluorescence in all channels used. Treatment with a lipofuscin autofluorescence quencher reduced autofluorescence, most efficiently in the near UV-area. These results, combined with the knowledge of the very active blood clearance function of LSECs support the notion that lysosomally located autofluorescent material reflected accumulation of lipofuscin in the intact liver. These results illustrate the importance of careful selection of fluorophores, especially when labelling of live cells where the quencher is not compatible.
|
|
| 5. |
- Lindström, Annika K, 1953-, et al.
(författare)
-
Immunohistochemical LRIG3 expression in cervical intraepithelial neoplasia and invasive squamous cell cervical cancer : association with expression of tumor markers, hormones, high-risk HPV-infection, smoking and patient outcome
- 2014
-
Ingår i: European journal of histochemistry. - : PAGEPress Publications. - 1121-760X .- 2038-8306. ; 58:2, s. 83-87
-
Tidskriftsartikel (refereegranskat)abstract
- The novel biomarker LRIG3 is a member of the LRIG family (LRIG1-3). While LRIG1 has been associated with favorable prognosis and LRIG2 with poor prognosis in invasive cervical cancer, little is known about the role of LRIG3. The aim of this study was to investigate the expression of LRIG3 in invasive cancer and cervical intraepithelial neoplasia (CIN) for possible correlation with other tumor markers, to hormones and smoking, as a diagnostic adjunct in CIN, and prognostic value in invasive cancer. Cervical biopsies from 129 patients with invasive squamous cell carcinoma and 170 biopsies showing low grade and high grade CIN, or normal epithelium were stained for LRIG3 and 17 additional tumor markers. Among other variables the following were included: smoking habits, hormonal contraceptive use, serum progesterone, serum estradiol, high-risk HPV-infection, meno pausal status and ten-year survival. In CIN, high expression of the tumor suppressors retinoblastoma protein, p53, and p16, and Ecadherin (cell-cell interaction), or low expression of CK10, correlated to LRIG3 expression. In addition, progestogenic contraceptive use correlated to high expression of LRIG3. In invasive cancer there was a correlation between expression of the major tumor promoter c-myc and high LRIG3 expression. High LRIG3 expression correlated significantly to presence of high-risk HPV infection in patients with normal epithelium and CIN. There was no correlation between LRIG3 expression and 10-year survival in patients with invasive cell cervical cancer. LRIG3 expression is associated with a number of molecular events in CIN. Expression also correlates to hormonal contraceptive use. The results on expression of other tumor markers suggest that LRIG3 is influ-markers in cancer and precancerous cells. Further studies are needed to elucidate if LRIG3 expression might be clinically useful.
|
|
| 6. |
- Nemolato, S., et al.
(författare)
-
Immunoreactivity for thymosin beta 4 and thymosin beta 10 in the adult rat oro-gastrointestinal tract
- 2013
-
Ingår i: European Journal of Histochemistry. - : PAGEPress Publications. - 1121-760X .- 2038-8306. ; 57:2, s. 106-111
-
Tidskriftsartikel (refereegranskat)abstract
- Thymosin beta 4 (T beta 4) and thymosin beta 10 (T beta 10) are two members of the beta-thymosin family, involved in multiple cellular activities in different organs in multiple animal species. Here we report the expression pattern of T beta 4 and T beta 10 in rat tissues, in the gut and in annexed glands. The two peptide were differently expressed: T beta 4 was absent in salivary glands whereas T beta 10 was expressed in parotid and in submandibular glands. T beta 4 was mildly expressed in the tongue and in the oesophagus, where T beta 10 was absent. A similar expression was found in the stomach, ileum and colon mucosa. In pancreas T beta 4 reactivity was restricted to the Langerhans islet cells; T beta 4 was also detected in the exocrine cells. Both peptide were not expressed in liver cells. When the rat expression pattern in rat organs was compared to reactivity for T beta 4 and T beta 10 in humans, marked differences were found. Our data clearly indicate a species-specific expression of T beta 4 and T beta 10, characterized by the actual unpredictability of the expression of these peptides in different cells and tissues. The common high expression of T beta 4 in mast cells, both in humans and in rats, represents one of the few similarities between these two species.
|
|
| 7. |
|
|
| 8. |
|
|
| 9. |
- Steinbeck, RG
(författare)
-
Dysplasia in view of the cell cycle
- 2004
-
Ingår i: European journal of histochemistry : EJH. - 1121-760X. ; 48:3, s. 203-211
-
Tidskriftsartikel (refereegranskat)
|
|
| 10. |
|
|
