| 1. |
- Abdel-Rehim, Abbi, et al.
(författare)
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Advantages of Saliva Sampling in Bioanalysis Using Microextraction by Packed Sorbent and Dried Saliva Spot with LC-MS-MS
- 2014
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Ingår i: LC GC Europe. - 1471-6577. ; 27:10, s. 529-531
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Tidskriftsartikel (refereegranskat)abstract
- Saliva offers a fast and non-invasive sampling matrix for determining drug concentration levels, making it a suitable alternative to plasma and blood. During the analysis of biological samples attention is focused on sample pre-treatment. In addition, liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) is often the method of choice in bioanalysis because of the good selectivity and good sensitivity of the technique. In this article, two sample handling and sample preparation methods for saliva samples are presented and discussed. The first method is microextraction by packed sorbent (MEPS), and the second method is dried saliva spot (DSS). Both methods were applied for determining the presence of lidocaine in saliva.
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| 2. |
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| 3. |
- Dejaegher, Bieke, et al.
(författare)
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Method development for drug impurity profiling : Part 1
- 2010
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Ingår i: LC GC Europe. - : Advanstar Communications. - 1471-6577. ; 23:4, s. 218-224
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Tidskriftsartikel (refereegranskat)abstract
- In this Practical Data Handling column, method development for drug impurity profiling is discussed. This first part consists of the first two steps when developing a drug impurity profile: the selection of a suitable chromatographic column and the optimization of the mobile phase pH. Prior to the impurity profiling, the selection of a set of dissimilar chromatographic columns is made (i.e., columns with different selectivities) to be screened with the impurity mixture for selectivity differences.
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| 4. |
- Fornstedt, Torgny
(författare)
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Modern supercritical fluid chromatography : Possibilities and pitfalls
- 2015
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Ingår i: LC GC Europe. - 1471-6577. ; 33:3, s. 166-174
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Tidskriftsartikel (refereegranskat)abstract
- There has been a revival of supercritical fluid chromatography (SFC) in recent years, especially in the chiral preparative field, but also more recently in the analytical area. However, SFC is considerably more complex than liquid chromatography (LC), mainly because of the compressibility of the mobile phase. One can say that SFC is a "€rubber variant of LC where everything considered constant in LC varies in SFC. In this review, we go through advances in theory, instrumentation, and novel applications.
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| 5. |
- Haglund, Peter, et al.
(författare)
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A modular approach to pressurized liquid extraction with in-cell clean-up
- 2010
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Ingår i: LC GC Europe. - Duluth, MN : Advanstar Communications, Inc.. - 1471-6577. ; 28:7, s. 544-
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Tidskriftsartikel (refereegranskat)abstract
- A new concept for the extraction of solid samples has been developed. The popular pressurized liquid extraction (PLE) technique has been modified so that the extraction cell can be divided, which greatly increases the flexibility and versatility of this technique. The system uses simple adaptors to couple commercially available extraction cells, which may be loaded with either sample or adsorbent(s). These segmented extraction cells make it possible to selectively retain analytes or matrix to achieve simultaneous extraction and clean-up or fractionation. The ultimate aim is to perform simultaneous extraction and clean-up and obtain a purified extract that is ready for instrumental analysis, all in line with current trends in analytical chemistry to automate and streamline the analytical procedures to reduce the analysis costs, solvent consumption, manual labour and increase the quality of analysis. Selected modes of operations are presented and a few recent environmental applications of modular PLE are reviewed.
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| 6. |
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| 7. |
- Jönsson, Jan Åke, et al.
(författare)
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Membrane extraction for sample preparation
- 2003
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Ingår i: LCGC Europe. - 1471-6577. ; 16:10, s. 683-683
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Tidskriftsartikel (refereegranskat)abstract
- Sample preparation techniques based upon liquid membrane extraction offer a high degree of selectivity and enrichment power and use small amounts of organic solvents. They also provide convenient possibilities for direct and automated interfacing with chromatography systems and other analytical instruments.
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| 8. |
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| 9. |
- Leeman, Mats, et al.
(författare)
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Analysis of Proteins, Biologics, and Nanoparticles in Biological Fluids Using Asymmetrical Flow Field-Flow Fractionation
- 2022
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Ingår i: LCGC Europe. - 1471-6577. ; 35:1, s. 7-13
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- With the increasing interest in biopharmaceuticals such as proteins, antibodies, and nucleic acids, there is a corresponding increase in the need for characterizing such components. Much effort is spent on characterization in the early drug development phases as well as during formulation development and quality control. One parameter that is commonly investigated is the size distribution of the macromolecular components to deduce if there is aggregation or degradation occurring, if conformational changes occur, or if there are interactions with excipients. While the properties of the protein drug in the buffer system or in the pharmaceutical formulation are important, possibly even more interesting are the properties of the drug once it enters the body. Size characterization of macromolecules in biological fluids has traditionally been an area hampered by the complexity of the matrix. The large amount of indigenous components can interfere with commonly applied analytical techniques for size characterization. However, the separation technique asymmetrical flow field-flow fractionation (AF4) has recently shown increasing applicability for the characterization of components in blood plasma and serum. This article reviews some aspects of applying AF4 to plasma, serum, milk, and cerebrospinal fluid in the field of analysis and characterization of proteins, biologics, and nanoparticles in biological fluids.
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| 10. |
- Leeman, Mats, et al.
(författare)
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Practical Applications of Asymmetrical Flow Field-Flow Fractionation (AF4): A Review
- 2015
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Ingår i: LCGC Europe. - 1471-6577. ; 28:12, s. 642-651
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Forskningsöversikt (refereegranskat)abstract
- Characterization of macromolecules and colloids is an area of considerable interest. Asymmetrical flow field-flow fractionation (AF4) has become a well-established method, but many potential users possess limited knowledge of its capabilities, or how it can provide additional information or serve as validation to the traditional analytical techniques. This review article highlights several practical applications where AF4 should be given special consideration, and discusses benefits and drawbacks of the different methods.
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