| 1. |
- Aebi, A, et al.
(författare)
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Microsatellite markers in a complex of Horismenus sp. (Hymenoptera : Eulophidae), parasitoids of bruchid beetles
- 2004
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Ingår i: Molecular Ecology Notes. - : Wiley. - 1471-8278 .- 1471-8286. ; 4:4, s. 707-709
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Tidskriftsartikel (refereegranskat)abstract
- Parasitoids of the genus Horismenus (Hymenoptera: Eulophidae) are the main natural enemies of bruchid beetles that feed on several species of Phaseolus beans. Samples of Horismenus depressus, H. missouriensis and H. butcheri were collected from seeds of three Phaseolus species throughout Mexico to examine the impact of plant variability on the genetic structure of parasitoid populations. For this purpose, six microsatellite loci were isolated and characterized. These loci are of great interest in understanding the taxonomy of the genus Horismenus, the most important Eulophid genus in the Neotropics.
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| 2. |
- Ahrén, Dag, et al.
(författare)
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PHOREST: a web-based tool for comparative analyses of expressed sequence tag data
- 2004
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Ingår i: Molecular Ecology Notes. - : Wiley. - 1471-8278 .- 1471-8286. ; 4:2, s. 311-314
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Tidskriftsartikel (refereegranskat)abstract
- Comparative analysis of expressed sequence tags is becoming an important tool in molecular ecology for comparing gene expression in organisms grown in certain environments. Additionally, expressed sequence tag database information can be used for the construction of DNA microarrays and for the detection of single nucleotide polymorphisms. For such applications, we present PHOREST, a web-based tool for managing, analysing and comparing various collections of expressed sequence tags. It is written in PHP (PHP: Hypertext Preprocessor) and runs on UNIX, Microsoft Windows and Macintosh (Mac OS X) platforms.
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| 3. |
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| 4. |
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| 5. |
- Nielsen, E E G, et al.
(författare)
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HYBRIDLAB (version 1.0): a program for generating simulated hybrids from population samples
- 2006
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Ingår i: Molecular Ecology Notes. - : Wiley. - 1471-8278 .- 1471-8286. ; 6:4, s. 971-973
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Tidskriftsartikel (refereegranskat)abstract
- We present the computer program HYBRIDLAB 1.0 for simulating intraspecific hybrids from population samples of nuclear genetic markers such as microsatellites, allozymes or SNPs ( single nucleotide polymorphisms). The program generates a user-specified number of multilocus F1 hybrid genotypes between any pair of potentially hybridizing populations included in a standard input-file of multilocus genotypes for population genetic analysis. This simple, user-friendly program has a wide range of applications for studying natural and artificial hybridization; in particular, for evaluating the statistical power for individual assignment of parental and hybrid individuals. An example of application for Atlantic cod populations is given.
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| 6. |
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| 7. |
- Andres, Olga, et al.
(författare)
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A microarray system for Y chromosomal and mitochondrial single nucleotide polymorphism analysis in chimpanzee populations
- 2008
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Ingår i: Molecular Ecology Notes. - : Wiley. - 1471-8278 .- 1471-8286 .- 1755-098X .- 1755-0998. ; 8:3, s. 529-539
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Tidskriftsartikel (refereegranskat)abstract
- Chimpanzee populations are diminishing as a consequence of human activities, and as a result this species is now endangered. In the context of conservation programmes, genetic data can add vital information, for instance on the genetic diversity and structure of threatened populations. Single nucleotide polymorphisms (SNP) are biallelic markers that are widely used in human molecular studies and can be implemented in efficient microarray systems. This technology offers the potential of robust, multiplexed SNP genotyping at low reagent cost in other organisms than humans, but it is not commonly used yet in wild population studies. Here, we describe the characterization of new SNPs in Y-chromosomal intronic regions in chimpanzees and also identify SNPs from mitochondrial genes, with the aim of developing a microarray system that permits the simultaneous study of both paternal and maternal lineages. Our system consists of 42 SNPs for the Y chromosome and 45 SNPs for the mitochondrial genome. We demonstrate the applicability of this microarray in a captive population where genotypes accurately reflected its large pedigree. Two wild-living populations were also analysed and the results show that the microarray will be a useful tool alongside microsatellite markers, since it supplies complementary information about population structure and ecology. SNP genotyping using microarray technology, therefore, is a promising approach and may become an essential tool in conservation genetics to help in the management and study of captive and wild-living populations. Moreover, microarrays that combine SNPs from different genomic regions could replace microsatellite typing in the future.
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| 8. |
- Griffith, Simon C., et al.
(författare)
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Fourteen polymorphic microsatellite loci characterized in the house sparrow Passer domesticus (Passeridae, Aves)
- 2007
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Ingår i: Molecular Ecology Notes. - : Wiley. - 1471-8278 .- 1471-8286. ; 7:2, s. 333-336
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Tidskriftsartikel (refereegranskat)abstract
- We characterized 14 polymorphic microsatellite loci in the house sparrow Passer domesticus. Four loci were isolated from house sparrow genomic libraries and 10 loci were identified by testing 100 loci that had been originally isolated in other passerine species. Loci were characterized in 37-54 unrelated sparrows from British and Norwegian populations. Each locus displayed between two and 31 alleles, with the observed heterozygosity ranging between 0.30 and 0.91.
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| 9. |
- Hailer, Frank, et al.
(författare)
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Development and multiplex PCR amplification of novel microsatellite markers in the White-tailed Sea Eagle, Haliaeetus albicilla (Aves: Falconiformes, Accipitridae)
- 2005
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Ingår i: Molecular Ecology Notes.. - : Wiley. - 1471-8278 .- 1471-8286. ; 5, s. 938-940
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Tidskriftsartikel (refereegranskat)abstract
- We report the development of 14 novel polymorphic microsatellite markers cloned from theWhite-tailed Sea Eagle, Haliaeetus albicilla, a formerly threatened raptor that has receivedmuch conservation attention throughout Eurasia. We also present a protocol for multiplexpolymerase chain reaction (PCR) amplification of the loci. Among 40 unrelated H. albicillaindividuals from southern Sweden, the markers produced two to eight alleles per locus,and average observed and expected heterozygosities were 0.463 and 0.468, respectively. Wefurther present five microsatellite markers that appeared monomorphic in H. albicilla, butwhich may be of interest for use in other raptor species
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| 10. |
- Hoffman, Eric A., et al.
(författare)
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Eleven polymorphic microsatellite loci in a coral reef fish, Pterapogon kauderni
- 2004
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Ingår i: Molecular Ecology Notes. - : Wiley. - 1471-8278 .- 1471-8286. ; 4:3, s. 342-344
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Tidskriftsartikel (refereegranskat)abstract
- We describe the isolation and characterization of 11 polymorphic tetranucleotide microsatellite loci from a male mouthbrooding coral reef fish, the Banggai cardinalfish Pterapogon kauderni. In a sample of 37 fish from a natural population, polymorphism at these loci ranged from two to 15 alleles, with expected heterozygosities ranging from 0.107 to 0.928, enabling high-resolution genetic studies of this coral reef fish.
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