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Sökning: L773:1532 4230 OR L773:1532 1819

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1.
  • Hansson, Lars-Olof, et al. (författare)
  • Comparison between Chicken and Rabbit Antibody Based Particle Enhanced Cystatin C Reagents for Immunoturbidimetry
  • 2008
  • Ingår i: Journal of immunoassay & immunochemistry. - : Informa UK Limited. - 1532-1819 .- 1532-4230. ; 29:1, s. 1-9
  • Tidskriftsartikel (refereegranskat)abstract
    • We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle-enhanced immunoassays.
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2.
  • Lönnroth, Ivar, 1940, et al. (författare)
  • Interaction Of Proteasomes And Complement C3, Assay Of Antisecretory Factor In Blood.
  • 2016
  • Ingår i: Journal of immunoassay & immunochemistry. - : Informa UK Limited. - 1532-1819 .- 1532-4230. ; 37:1, s. 43-54
  • Tidskriftsartikel (refereegranskat)abstract
    • Antisecretory factor (AF) is a protein complex which inhibits inflammation and regulates fluid transport. In the present study two new immunoassays (ELISA) are developed. The first ELISA establishing a 26S proteasome concentration of 0.41±0.03 µg/ml in normal plasma, the second ELISA disclosing the binding of proteasomes to complement factor C3. The latter test values increased about tenfold following intake of processed cereals, paralleling with the old AF ELISA. The proteasome/C3 complex is purified and shown to expose hidden antisecretory peptide sequence and contain the inactive C3c protein. These findings might explain the antisecretory and anti-inflammatory effect during AF complex formation.
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  • Resultat 1-4 av 4

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