| 1. |
- Aghabagherzadeh, Mozhdeh, et al.
(författare)
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Folic Acid-Conjugated Chitosan-Coated Solid Lipid Nanoparticles: Precision Targeting of Artemisia vulgaris Essential Oils for Anticancer Therapy
- 2024
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Ingår i: Chemistry and Biodiversity. - 1612-1880 .- 1612-1872. ; 21:1
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Tidskriftsartikel (refereegranskat)abstract
- In this study, we developed Solid Lipid Nanoparticles (SLN-NPs) loaded with Artemisia vulgaris essential oil and coated with folic acid-chitosan (AVEO-SCF-NPs) to enhance drug delivery in biotechnology and pharmaceutical sectors. AVEO-SCF-NPs were synthesized using homogenization and ultra-sonication methods and comprehensively characterized. These nanoparticles exhibited a particle size of 253.67 nm, Polydispersity Index (PDI) of 0.26, zeta potential (zeta-p) of +39.96 mV, encapsulation efficiency (%EE) of 99.0 %, and folic acid binding efficiency (% FB) of 46.25 %. They effectively inhibited MCF-7, HT-29, and PC-3 cancer cells with IC50 values of 48.87 mu g/mL, 88.48 mu g/mL, and 121.34 mu g/mL, respectively, and demonstrated antibacterial properties against Gram-positive strains. AVEO-SCF-NPs also exhibited scavenging effects on ABTS (IC50: 203.83 mu g/mL) and DPPH (IC50: 680.86 mu g/mL) free radicals and inhibited angiogenesis, as confirmed through CAM and qPCR assays. Furthermore, these nanoparticles induced apoptosis, evidenced by up-regulation of caspase 3 and 9, down-regulation of TNF-alpha genes, and an increase in SubG1 phase cells. The high loading capacity of SCF-NPs for AVEO, coupled with their multifaceted biological properties, highlights AVEO-SCF-NPs as promising candidates for cancer therapy in the biotechnology and pharmaceutical industries.
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| 2. |
- Andersson, Charlotta S., 1979-, et al.
(författare)
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A dynamic c terminal segment in the mycobacterium tuberculosis mn/fe r2lox protein can adopt a helical structure with possible functional consequences
- 2012
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Ingår i: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 9:9, s. 1981-1988
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Tidskriftsartikel (refereegranskat)abstract
- Mycobacterium tuberculosis R2-like ligand-binding oxidase (MtR2lox) belongs to a recently discovered group of proteins that are homologous to the ribonucleotide reductase R2 proteins. MtR2lox carries a heterodinuclear Mn/Fe cofactor and, unlike R2 proteins, a large ligand-binding cavity. A unique tyrosine-valine cross link is also found in the vicinity of the active site. To date, all known structures of R2 and R2lox proteins show a disordered C-terminal segment. Here, we present two new crystal forms of MtR2lox, revealing an ordered helical C-terminal. The ability of alternating between an ordered and disordered state agrees well with bioinformatic analysis of the protein sequence. Interestingly, ordering of the C-terminal helix shields a large positively charged patch on the protein surface, potentially used for interaction with other cellular components. We hypothesize that the dynamic C-terminal segment may be involved in control of protein function in vivo.
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| 3. |
- Danieli, B, et al.
(författare)
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Synthesis and biological evaluation of paclitaxel-thiocoichicine hybrids
- 2004
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Ingår i: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 1:2, s. 327-345
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Tidskriftsartikel (refereegranskat)abstract
- The bifunctional taxoid-colchicinoid hybrids 6-8 were synthesized and evaluated in assays of cytotoxicity and tubulin assembly/disassembly. All compounds showed a high degree of cytotoxicity, but, while 6 and 7 behaved as bifunctional tubulin binders not unlike an equimolecular mixture of taxol and thiocolchicine, 8 was surprisingly devoid of tubulin activity, acting on a distinct and yet to identify molecular target.
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| 4. |
- Farida, Seyed Hamed Moazzami, et al.
(författare)
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Fatty Acid Patterns of Seeds of Some Salvia Species from Iran : A Chemotaxonomic Approach
- 2016
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Ingår i: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 13:4, s. 451-458
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Tidskriftsartikel (refereegranskat)abstract
- In this study, the seed oil content and fatty acid (FA) profile of 21 populations from 16 wild Salvia species of Iran were analyzed by GC. Patterns of chemical variations of the oils among species were identified via numerical analyses and also the taxonomic status of the infrageneric grouping was outlined in the genus. Salvia species were scored based on the contents of main FAs using principal coordinate analysis (PCO). The results showed that the total oil content in the seeds varied significantly, and ranged from 6.68 to 38.53% dry weight. alpha-Linolenic (18:3 omega 3, 1.69 -53.56%), linoleic (18:2 omega 6, 13.04 - 60.64%), oleic (18:1 omega 9, 6.15 - 27.06%), palmitic (16:0, 3.77-9.27%), and stearic (18:0, 1.78 - 3.05%) acid were identified as five major FAs in the oils. The amount of omega-3 and omega-6 FAs ranged between 1.90 - 53.80% and 13.46 - 60.83% of total FAs in the seed oils, respectively. The results confirmed that FA profiles were distinctive among the species and that they can be used as chemotaxonomic markers. The discrimination of Salvia species according to their botanical classification at intersectional level was supported. In general, seed oils of Salvia species were rich sources of polyunsaturated FAs, except in linoleic and alpha-linolenic acid, and may be valuable for food and pharmaceutical industries.
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| 5. |
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| 6. |
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| 7. |
- Mottaghipisheh, Javad
(författare)
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A Review on Torilis japonica: Ethnomedicinal, Phytochemical, and Biological Features
- 2023
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Ingår i: Chemistry & Biodiversity. - 1612-1872 .- 1612-1880. ; 20
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Forskningsöversikt (refereegranskat)abstract
- The present study was aimed at comprehensive overviewing a phytochemically and biologically important species namely Torilis japonica (Apiaceae family). Treatment of dysentery, fever, haemorrhoids, spasm, uterine tumors, lymphadenitis, rheumatism, impotence, infertility, women's diseases, and chronic diarrhea are reported as the main folk medicinal applications of the T. japonica fruits. So far, the plant is phytochemically characterized for its diverse terpene derivatives, predominantly sesquiterpenes. The plant's fruit is a rich source of torlin, a guaiane-type sesquiterpene, possessing various potent bioactivities. To date, anticancer, anti-inflammatory, antimicrobial, antioxidant, skin photoaging activities of the plant extracts and its constituents have been evaluated. Further investigation of the plant, specifically bioassay-guided isolation and identification of its major bioactive constituents can lead to discover potential phytopharmaceutical candidates.
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| 8. |
- Norden, B, et al.
(författare)
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Analysis and understanding of high-dimensionality data by means of multivariate data analysis
- 2005
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Ingår i: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 2:11, s. 1487-1494
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Tidskriftsartikel (refereegranskat)abstract
- Multivariate analysis such as principal-components analysis (PCA) and partial-least-squares-discriminant analysis (PLS-DA) have been applied to peptidomics data from clinical urine samples subjected to LC/MS analysis. We show that it is possible to use these methods to get information from a complex set of clinical data. The aim of the work is to use this information as a first step in the further search for clinical biomarker data. It is possible to identify peptide-biomarker fingerprints related to disease diagnosis and progression. Further, we review clinical proteomics and pharmacogenomics data analyzed with the same multivariate approach.
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| 9. |
- Pathmasiri, Wimal, et al.
(författare)
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Aryl ketones from Acronychia pedunculata with cyclooxygenase-2 inhibitory effects
- 2005
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Ingår i: Chemistry & biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 2:4, s. 463-469
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Tidskriftsartikel (refereegranskat)abstract
- 1-[2,4-Dihydroxy-6-methoxy-3,5-bis(3-methylbut-2-en-1-yl)phenyl]ethanone (1), and a new aryl ketone, named acrovestenol (2), were isolated as cyclooxygenase-2 (COX-2) inhibitory principles from a CH2Cl2 extract of the bark of Acronychia pedunculata by a bioassay-guided fractionation procedure. Compound 2 inhibited COX-2 with an IC50 value of 142.0+/-2.15 microM, compared to the COX-2 inhibitory reference compound NS-398 with an IC50 value of 11.3+/-1.12 microM. Compound 1 inhibited COX-2-catalyzed PG biosynthesis with 68% at a concentration of 500 microM. The structures were determined by UV, IR, and 1D- and 2D-NMR, including TOCSY, HSQC-DEPT, and HMBC, and MS investigations.
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| 10. |
- Staples, Richard, et al.
(författare)
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Structure and Chemical Analysis of Major Specialized Metabolites Produced by the Lichen Evernia prunastri
- 2020
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Ingår i: Chemistry and Biodiversity. - : Wiley. - 1612-1872 .- 1612-1880. ; 17:1
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Tidskriftsartikel (refereegranskat)abstract
- We performed comparative profiling of four specialized metabolites in the lichen Evernia prunastri, collected at three different geographic locations, California and Maine, USA, and Yoshkar Ola, Mari El, Russia. Among the compounds produced at high concentrations that were identified in all three specimens, evernic acid, usnic acid, lecanoric acid and chloroatranorin, evernic acid was the most abundant. Two depsidones, salazinic acid and physodic acid, were detected in the Yoshkar‐Ola collection only. The crystalline structure of evernic acid (2‐hydroxy‐4‐[(2‐hydroxy‐4‐methoxy‐6‐methylbenzoyl)oxy]‐6‐methylbenzoate) (hmb) revealed two crystallographically and conformationally distinct hmb anions, along with two monovalent sodium atoms. One hmb moiety contained an exotetradentate binding mode to sodium, whereas the other exhibited an exohexadentate binding mode to sodium. Embedded edge‐sharing {Na2O8}n sodium‐oxygen chains connected the hmb anions into the full three‐dimensional crystal structure of the title compound. The crystal used for single‐crystal X‐ray diffraction exhibited non‐merohedral twinning. The data suggest the importance of the acetyl‐polymalonyl pathway products to processes of maintaining integrity of the lichen holobiont community.
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