| 1. |
- Al Haj, Mahmoud, et al.
(författare)
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Changes in insulin-like growth factor-1 and IGF-binding protein-3 in camel plasma during dehydration in the presence and absence of losartan
- 2012
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Ingår i: Comparative Clinical Pathology. - : Springer Science and Business Media LLC. - 1618-5641 .- 1618-565X. ; 21:6, s. 1745-1749
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Tidskriftsartikel (refereegranskat)abstract
- In the present study, the effect of 20 days of dehydration in the presence or absence of losartan (angiotensin II AT1 receptor antagonist) on insulin-like growth factor-1(IGF-1) and insulin-like growth factor-binding protein-3(IGFBP-3) in plasma of the one-humped camel was studied. Eighteen male camels, 3-4 years of age, were divided into three equal groups: control, dehydrated, and dehydrated-losartan-treated groups. The control camels were given food and water ad libitum. The two dehydrated groups underwent 20 days of water deprivation but were given food ad libitum. The dehydrated-losartan-treated camels were given losartan injection (Merck, USA), intravenously at a dose of 5 mg/kg body weight daily for 20 days. Our results demonstrated a progressive decrease in the circulating levels of IGF-1 and IGFBP-3 in the dehydrated and dehydrated-losartan-treated animals across dehydration compared to their basal levels and time-matched control. On day 5 of dehydration, the IGF-1 level in the losartan-treated group showed a decrease of 60 % and the dehydrated group showed 45 % decrease from their baseline levels and time-matched control. On day 10 the decrease in the losartan-treated animals reached 74 % and for the dehydrated was 62 %. On day 20 the decrease in the losartan-treated was 89 % and for the dehydrated reached 80 % from their baseline levels and time-matched control. Dehydration in the presence or absence of losartan caused a decrease in the circulating level of IGFBP-3. The decrement reached 26 % on day 10 and 20 for the treated camels, while the decrease for the dehydrated was 22 % on day 10 of dehydration and reached 29 % on day 20 compared to their baseline levels and time-matched control. In conclusion, dehydration alone, or in presence of Angiotensin II AT1 receptor blocker caused significant decrease in the circulating levels of IGF-1 and IGFBP-3 compared to their basal values and to time-matched controls. Losartan enhanced the effect of dehydration mainly in the early phase of dehydration for both parameters; albeit, no significant differences between the two dehydrated groups was observed. Finally, these findings suggest an essential role of IGF-1and IGFBP-3 in the dehydration state of these dromedarian camels.
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| 2. |
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| 3. |
- Pettersson, Helena, et al.
(författare)
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Effect of 1 mg/kg oral prednisolone on biochemical analytes in ten dogs: a cross‑over study
- 2021
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Ingår i: Comparative Clinical Pathology. - : Springer Science and Business Media LLC. - 1618-5641 .- 1618-565X. ; 30, s. 519-528
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Tidskriftsartikel (refereegranskat)abstract
- Prednisolone is used for treatment of inflammatory, allergic, neoplastic, and immune-mediated diseases in dogs. As a glucocorticoid, prednisolone has biochemical effects, which may interfere with the interpretation of biochemistry test results. The aim of this study is to investigate the effects of prednisolone treatment in an anti-inflammatory dose on common biochemical analytes in dogs and to evaluate the clinical relevance of the changes. Ten beagle dogs, enrolled in a cross-over study, were treated with oral prednisolone (1 mg/kg 24 h) for 10 days. Blood samples were collected at day 0, 1, 3, 6, 9, 10, 12, 16, and 20. Data was analyzed using a general linear model with time and treatment as fixed factors. Pairwise comparisons were done between prednisolone and control period for each dog and sampling. Significant results were further evaluated for clinical relevance using laboratory-specific reference intervals and reference change values (RCVs), when available. Statistically significant changes were observed for ALP activity and iron concentration, which increased to levels exceeding the RCV, and several results were outside reference intervals. Phosphate and bile acids increased significantly, while amylase, lipase, and cholesterol decreased significantly, but with mean/median results remaining within reference intervals. Anti-inflammatory prednisolone treatment did not induce significant changes in ALT, GLDH, GGT, cPLI, glucose, or calcium. Treatment with an anti-inflammatory dose of prednisolone induced changes in several analytes. Only the increases in ALP and iron were of such magnitude that they are expected to affect the clinical interpretation of test results.
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| 4. |
- Torsson, Emeli, et al.
(författare)
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Detection of peste des petits ruminants and concurrent secondary diseases in sheep and goats in Ngorongoro district, Tanzania
- 2019
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Ingår i: Comparative Clinical Pathology. - : Springer Science and Business Media LLC. - 1618-5641 .- 1618-565X. ; 28, s. 755-759
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Tidskriftsartikel (refereegranskat)abstract
- Small ruminants play an important role in the livelihoods of resource-constrained communities. This study was initiated because of a massive outbreak of a respiratory disease in sheep and goats in Loliondo area in Ngorongoro district of Arusha region in Tanzania in 2016. During flock examination, a total of 240 serum samples and 61 nasal swabs were collected. Antibodies to small ruminant morbillivirus, causative agent of peste des petits ruminants (PPR), were detected from sera using a competitive enzymelinked immunosorbent assay. A multiplex reverse transcription real-time polymerase chain reaction assay was used to detect four pathogens: small ruminant morbillivirus, Mycoplasma capricolum subspecies capripneumoniae, Pasteurella multocida, and Capripoxvirus from the nasal swabs. Overall seroprevalence of PPR was 74.6%, with all four pathogens detected from nasal swabs. Co-infections of small ruminant morbillivirus and Mycoplasma capricolum subspecies capripneumoniae, small ruminant morbillivirus and Capripoxvirus, small ruminant morbillivirus and Pasteurella multocida, and Mycoplasma capricolum subspecies capripneumoniae and Capripoxvirus were also detected. Presence of PPR and the other diseases in this study provided insight into the severity of the outbreak in sheep and goats in Ngorongoro district. Thus, laboratory confirmation is critical for prompt and appropriate interventions to be made for control of diseases in sheep and goats with similar clinical signs. The findings also call for research into development of combined vaccines targeting common diseases of small ruminants in Tanzania.
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| 5. |
- Tvedten, Harold
(författare)
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Megakaryocyte cytoplasmic fragments and ragocytes in the peripheral blood of a domestic cat infected with Anaplasma
- 2023
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Ingår i: Comparative Clinical Pathology. - : Springer Science and Business Media LLC. - 1618-5641 .- 1618-565X. ; 32, s. 329-333
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Tidskriftsartikel (refereegranskat)abstract
- In this case report, the author reports three strong and highly unusual changes in the peripheral blood of a domestic cat infected with Anaplasma. A freshly made blood smear from the cat Anaplasma had a shower of about 100 variably large to huge cytoplasmic fragments of megakaryocyte cytoplasm along the feathered edge. These had budding of platelets at their margins. Neutrophils with red cytoplasmic inclusions resembling ragocytes and neutrophils with ingested round inclusions of nuclear material were seen in a 1-day-old EDTA blood sample but not the freshly made blood smear. The cells with nuclear material resembled LE cells or alternatively Tart cells.
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| 6. |
- Öberg, Josefine, et al.
(författare)
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Evaluation of an equine-optimized enzyme-linked immunosorbent assay for serum insulin measurement and stability study of equine serum insulin
- 2012
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Ingår i: Comparative Clinical Pathology. - : Springer Science and Business Media LLC. - 1618-5641 .- 1618-565X. ; 21, s. 1291-1300
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Tidskriftsartikel (refereegranskat)abstract
- This study aims to evaluate a commercially available equine-optimized insulin assay and to evaluate the stability of equine insulin. In addition, serum insulin concentrations before and after feeding are also presented. Samples were taken before and after feeding from 40 healthy horses and from 15 equine patients visiting the University Equine Hospital. Insulin was analysed with the equine ELISA and with two human methods (one ELISA and one RIA). Precision was determined by repeated analysis of samples on one assay run and from one sample analysed on 15 different assays. Recovery from two dilution series and from an additional study was evaluated. Stability of equine insulin was evaluated in samples with and without haemolysis stored at 18–20°C, 6–8°C for 30 days and at −20°C for 1 year. The equine assay correlated well with both human assays (r 2 = 0.97 for both assays). The intra-assay coefficient of variance (CV) was 2.0–6.5%, and the inter-assay CV was 10.7%. Recovery upon dilution was 82–100%, and recovery upon addition was 102–115%. There was no significant decrease in insulin concentrations for non-haemolyzed samples when stored at 6–8°C for 30 days or at −20°C for 1 year. Mean insulin concentration was significantly higher (347 ng/L) after feeding compared with before feeding (123 ng/L). The equine assay correlated well with the previously used assays. The assay had good precision and recovery after dilution and addition. A significant increase in mean insulin concentration was seen in horses after feeding
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| 7. |
- Öberg, Josefine, et al.
(författare)
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Thromboelastography in a cat with steroid responsive thrombocytopenia
- 2010
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Ingår i: Comparative Clinical Pathology. - 1618-5641 .- 1618-565X. ; 19, s. 429-431
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Tidskriftsartikel (refereegranskat)abstract
- Abstract A Devon Rex cat was presented for bruises, petechie, and ecchymoses. Marked thrombocytopenia was the only primary abnormality noted on initial hematology and other testing. Thromboelastography and clinical signs supported the conclusion that this was a true thrombocytopenia. Use of optical platelet counting to detect large platelets and prostaglandin E1 to prevent platelet aggregation ruled out pseudothrombocytopenia. Exclusion of likely causes of thrombocytopenia and a rapid response to immunosuppressive doses of prednisolone suggested this was an immune-mediated thrombocytopenia. Signs of bleeding returned when the prednisolone dosage was decreased, and the platelet count again increased rapidly to an increased dosage. Thromboelastography showed marked hypocoagulability and may be useful to differentiate true thrombocytopenia from pseudothrombocytopenia, which occurs commonly in cats
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