| 1. |
- Greaves, Ronda F, et al.
(författare)
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The IFCC Curriculum - phase 1.
- 2018
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Ingår i: EJIFCC. - 1650-3414. ; 29:1, s. 55-93
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Tidskriftsartikel (refereegranskat)
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| 2. |
- Grubb, Anders
(författare)
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Cystatin C is Indispensable for Evaluation of Kidney Disease
- 2017
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Ingår i: EJIFCC. - 1650-3414. ; 28:4, s. 268-276
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Forskningsöversikt (refereegranskat)abstract
- The present minireview of the place of cystatin C in clinical medicine emphasizes, and discuss the evidence, that cystatin C-based GFR-estimating equations do not require the use of vague terms like race and sex, that cystatin C-based GFR-esti mating equations are useful for both children and adults, including the elderly, that the best GFR-estimation requires simultaneous use of both cystatin C- and creatinine-based equations, that cystatin C-based GFR-estimating equations are superior to creatinine-based equations in predicting end-stage renal disease, cardiovascular manifestations, hospitalisation and death, and, finally that cystatin C is required to diagnose the new syndrome "Shrunken Pore Syndrome" with its high mortality and morbidity, even in the absence of reduced GFR. When automated laboratory equipment is available, the cost of cystatin C is comparable to that of enzymatically determined creatinine. The conclusion is that cystatin C should be used at least as often as creatinine in clinical medicine.
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| 3. |
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| 4. |
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| 5. |
- Pollak, Joanna, et al.
(författare)
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Production of Cystatin C Wild Type and Stabilized Mutants
- 2010
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Ingår i: EJIFCC. - 1650-3414. ; 20:4, s. 70-166
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Tidskriftsartikel (refereegranskat)abstract
- Cystatin C is produced in all nucleated cells. It has various functions and biological activities. Researchers are focused on its role in kidney diseases as a marker of glomerular filtration but also as a very important link in development of amyloid diseases. This work describes expression and purification of both wild type (wt) and stabilized form (stab 1 and 2) of wt cystatin C and amyloid-forming L68Q mutant of cystatin C. The recombinant cystatin C can be used in projects requiring pure cystatin C to examine models of dimerization and fibrils formation as well as a standard in clinical tests.
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| 6. |
- Trzybulska, Dorota, et al.
(författare)
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MiRNA and other non-coding RNAs as promising diagnostic markers
- 2018
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Ingår i: Electronic Journal of the International Federation of Clinical Chemistry and Laboratory Medicine. - 1650-3414. ; 29:3, s. 221-226
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Tidskriftsartikel (refereegranskat)abstract
- Since the discovery of non-coding RNAs (ncRNAs) a new area has emerged in the field of biomarkers. NcRNAs are RNA molecules of different sizes that are transcribed as independent genes or as part of protein coding genes and are not translated, therefore they do not produce proteins. They have been classified accord-ing to their size and function and include micro RNAs (miRNAs), piwiRNAs (piRNAs), snoRNAs and long non-co ding RNAs (lncRNAs). These non-coding RNAs are present in different cell compartments participating in multiple cell functions, but they have also been identified in biological fluids, also known as cell-free or circulating ncRNAs, where they can be detected in exosomes, bound on lipoproteins as well as free circu-lating molecules. The role of circulating ncRNAs is still under investigation but are believed to be paracrine or endocrine messengers to systematically deliver signals between cells and tissues. Detecting ncRNAs in biological fluids has opened a new field in Clinical Chemistry utilizing them as biomarkers of diseases or prognostic markers for different pathological condi-tions. Herein, the different types of ncRNAs and their potential in the field of diagnostics are outlined.
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