| 1. |
- Abellan-Flos, Marta, et al.
(författare)
-
QCM sensing of multivalent interactions between lectins and well-defined glycosylated nanoplatforms
- 2019
-
Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 139
-
Tidskriftsartikel (refereegranskat)abstract
- Quartz crystal microbalance (QCM) methodology has been adopted to unravel important factors contributing to the "cluster glycoside effect" observed in carbohydrate-lectin interactions. Well-defined, glycosylated nanostructures of precise sizes, geometries and functionalization patterns were designed and synthesized, and applied to analysis of the interaction kinetics and thermodynamics with immobilized lectins. The nanostructures were based on Borromean rings, dodecaamine cages, and fullerenes, each of which carrying a defined number of carbohydrate ligands at precise locations. The synthesis of the Borromeates and dodecaamine cages was easily adjustable due to the modular assembly of the structures, resulting in variations in presentation mode. The binding properties of the glycosylated nanoplatforms were evaluated using flow-through QCM technology, as well as hemagglutination inhibition assays, and compared with dodecaglycosylated fullerenes and a monovalent reference. With the QCM setup, the association and dissociation rate constants and the associated equilibrium constants of the interactions could be estimated, and the results used to delineate the multivalency effects of the lectin-nanostructure interactions.
|
|
| 2. |
- Abrahamsson, D, et al.
(författare)
-
A preliminary study on DNA detection based on relative magnetic permeability measurements and histone HI conjugated superparamagnetic nanoparticles as magnetic tracers
- 2004
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 19:11, s. 1549-1557
-
Tidskriftsartikel (refereegranskat)abstract
- Histone H I conjugated superparamagnetic nanoparticles were assessed for their ability to work as magnetic tracers in conjunction with the relative magnetic permeability metre (MPM-100) for the detection and quantification of DNA (deoxyribonucleic acid). The method employed was based on the electrostatic adsorption of DNA (analyte) to amino group derivatised silica (carrier) and subsequent binding of histone HI conjugated superparamagnetic nanoparticles (magnetic tracer). The sandwich complexes formed were separated from the medium by sedimentation and the relative magnetic permeability of the sediments were measured with the MPM-100. Investigations were made with both calf thymus DNA and plasmid DNA in aqueous buffered solution as well as in a lysed cell culture with high protein content. For the quantification of calf thymus DNA, a linear relationship between the DNA concentration in the sample and the relative magnetic permeability of the pellet was found for DNA concentrations up to 67 mug/ml in buffered solutions as well as in a lysed cell culture. The limits of detection were determined to 12 and 31 mug/ml, respectively. For the quantification of plasmid DNA in buffered solution a linear range was established for concentrations in up to 150 VLg/ml and the limit of detection was determined to 52 mug/lnl.
|
|
| 3. |
|
|
| 4. |
- Ahamadzadeh, Ezat, et al.
(författare)
-
Automated analysis of human cardiomyocytes dynamics with holographic image-based tracking for cardiotoxicity screening
- 2022
-
Ingår i: Biosensors & bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 195
-
Tidskriftsartikel (refereegranskat)abstract
- This paper proposes a new non-invasive, low-cost, and fully automated platform to quantitatively analyze dynamics of human-induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs) at the single-cell level by holographic image-based tracking for cardiotoxicity screening. A dense Farneback optical flow method and holographic imaging informatics were combined to characterize the contractile motion of a single CM, which obviates the need for costly equipment to monitor a CM's mechanical beat activity. The reliability of the proposed platform was tested by single-cell motion characterization, synchronization analysis, motion speed measurement of fixed CMs versus live CMs, and noise sensitivity. The applicability of the motion characterization method was tested to determine the pharmacological effects of two cardiovascular drugs, isoprenaline (166 nM) and E−4031 (500 μM). The experiments were done using single CMs and multiple cells, and the results were compared to control conditions. Cardiomyocytes responded to isoprenaline by increasing the action potential (AP) speed and shortening the resting period, thus increasing the beat frequency. In the presence of E−4031, the AP speed was decreased, and the resting period was prolonged, thus decreasing the beat frequency. The findings offer insights into single hiPS-CMs’ contractile motion and a deep understanding of their kinetics at the single-cell level for cardiotoxicity screening.
|
|
| 5. |
- Ahmad, Farook, et al.
(författare)
-
Minimizing tissue-material interaction in microsensor for subcutaneous glucose monitoring
- 2007
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 22:8, s. 1625-1632
-
Tidskriftsartikel (refereegranskat)abstract
- A new implantable electrocatalytic glucose sensor for subcutaneous glucose monitoring has been fabricated by immobilizing glucose oxidase on a chemically modified carbon fiber. The sensor was inserted subcutaneously on a male spraguely rat without any incision after dipping the microsensor in the rat's serum for 3 days. The so called "stained" microsensor, operated in the amperometric mode with an applied potential of +0.23 V versus Ag vertical bar AgCl, was able to directly measure the glucose concentration upon infusion of glucose. The results obtained were encouraging, with the response time was less than 2 s and the apparent Michaelis-Menten value at 5.1 +/- 0.5 mM. The "stained" microsensor shows good stability and reproducibility with constant response spanned over 25 days. Most common interferences in glucose analysis were minimized by the outerlayer Nafion((R)). Hematology examinations showed minimal material-tissue interaction. Use of such mechanical devices will allow a more refined understanding towards glucose control in diabetic patients as the implanted microsensor was not effected by biocompatibility failures. (c) 2006 Elsevier B.V. All rights reserved.
|
|
| 6. |
- Akkilic, Namik, et al.
(författare)
-
Single-molecule biosensors: Recent advances and applications
- 2020
-
Ingår i: Biosensors and Bioelectronics. - : Elsevier BV. - 0956-5663 .- 1873-4235. ; 151
-
Forskningsöversikt (refereegranskat)abstract
- Single-molecule biosensors serve the unmet need for real time detection of individual biological molecules in the molecular crowd with high specificity and accuracy, uncovering unique properties of individual molecules which are hidden when measured using ensemble averaging methods. Measuring a signal generated by an individual molecule or its interaction with biological partners is not only crucial for early diagnosis of various diseases such as cancer and to follow medical treatments but also offers a great potential for future point-of-care devices and personalized medicine. This review summarizes and discusses recent advances in nanosensors for both in vitro and in vivo detection of biological molecules offering single-molecule sensitivity. In the first part, we focus on label-free platforms, including electrochemical, plasmonic, SERS-based and spectroelectrochemical biosensors. We review fluorescent single-molecule biosensors in the second part, highlighting nanoparticle-amplified assays, digital platforms and the utilization of CRISPR technology. We finally discuss recent advances in the emerging nanosensor technology of important biological species as well as future perspectives of these sensors.
|
|
| 7. |
|
|
| 8. |
- Alcock, S. J., et al.
(författare)
-
Advances in the use of in vivo sensors
- 1992
-
Ingår i: Biosensors & bioelectronics. - : Elsevier. - 0956-5663 .- 1873-4235. ; 7:4, s. 243-254
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- The sixth workshop of the European Community Concerted Action on Chemical sensors for in vivo monitoring was held at Snogeholm, Sweden, in October 1991. The meeting reviewed recent in vivo and ex vivo results, and also included a shorter session on ethical and safety problems.
|
|
| 9. |
- ALCOCK, SJ, et al.
(författare)
-
THE DESIGN AND DEVELOPMENT OF NEW CHEMICAL SENSORS FOR INVIVO MONITORING
- 1991
-
Ingår i: Biosensors & bioelectronics. - : Elsevier Science B.V., Amsterdam.. - 0956-5663 .- 1873-4235. ; 6:8, s. 647-652
-
Tidskriftsartikel (refereegranskat)abstract
- The latest workshop of the European Community (EC) Concerted Action on Chemical sensors for in vivo monitoring was held in Nauplion, Greece, in April this year. This fifth workshop focused on The design and development of new sensors for in vivo monitoring, and was organized into five sessions: design and development of new sensors; operational considerations; performance of analytical systems; novel sensors/tissue heterogeneity; and infra-red spectroscopy.
|
|
| 10. |
- Allender, Chris, et al.
(författare)
-
MIP2008 Kobe, Japan, September 2008 preface
- 2009
-
Ingår i: Biosensors & Bioelectronics. - : Elsevier BV. - 1873-4235 .- 0956-5663. ; 25:3, s. 539-542
-
Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)
|
|
